43163 2017 eng 1 2 other Knauer Berlin 0 -- -- -- The use of antibodies is becoming more and more important. Therewith the need for fast and robust methods for their purification and columns with a long lifetime is growing. In this application, we describe a fast and robust protein A affinity chromatography method with a new monolithic column. Antibodies were successfully purified in less than 1.5 minutes with a flowrate of 5 mL/min highlighting the advantages of this column and method. Knauer Application Sheet http://www.knauer.net/en/application/fast_and_robust_purification_of_antibodies_from_human_serum_with_a_new_monolithic_protein_a_column.html U. Krop Franziska Scherz Michael G. Weller K. Monks eng uncontrolled Antibodies eng uncontrolled Protein A eng uncontrolled MNPQ eng uncontrolled Technology transfer eng uncontrolled Human serum eng uncontrolled Purification eng uncontrolled IgG eng uncontrolled SDS PAGE eng uncontrolled Regeneration Analytische Chemie Datei im Netzwerk der BAM verfügbar ("Closed Access") Graue Literatur