40062 2017 eng Article 21, 1 11 15 article 1 -- -- -- Quantification of nanoparticle (NP) uptake in cells or tissues is very important for safety assessment. Often, electron microscopy based approaches are used for this purpose, which allow imaging at very high resolution. However, precise quantification of NP numbers in cells and tissues remains challenging. The aim of this study was to present a novel approach, that combines precise quantification of NPs in individual cells together with high resolution imaging of their intracellular distribution based on focused ion beam/ scanning electron microscopy (FIB/SEM) slice and view approaches. Journal of Nanobiotechnology 10.1186/s12951-017-0255-8 1477-3155 urn:nbn:de:kobv:b43-400626 03.05.2017 Creative Commons - Namensnennung 3.0 E. Guehrs M. Schneider Ch. M. Günther P. Hessing K. Heitz D. Wittke Ana López-Serrano Oliver Norbert Jakubowski J. Plendl S. Eisebitt A. Haase eng uncontrolled Nanoparticles eng uncontrolled FIB/SEM slice and view eng uncontrolled Absolute dose eng uncontrolled Cellular internalization eng uncontrolled Macrophage Analytische Chemie Verlagsliteratur Datei für die Öffentlichkeit verfügbar ("Open Access") Wissenschaftliche Artikel der BAM Bundesanstalt für Materialforschung und -prüfung (BAM) https://opus4.kobv.de/opus4-bam/files/40062/FIB_SEM.pdf