40056 2017 eng lecture 0 -- -- -- Multiparametric analyses involving optical techniques like flow cytometry are at the core of studying complex systems in biological research and diagnostic applications. However, for fluorescence-based techniques, the number of reporters distinguishable in spectral multiplexing is limited by spectral overlap and requires a multitude of excitation light sources and detection Systems. Intensity encoding often used for bead assays suffers from problems regarding dye concentration control and excitation light intensity fluctuations. An alternative is luminescence lifetime encoding, particularly to minimize instrument costs. Here, we report on dye-stained polymer microparticles for lifetime encoding in flow cytometry with different organic dyes. This ranges from studies of the impact of parameters like dye loading concentration and particle diameter on fluorescence decay behavior to the demonstration of lifetime code reading and simultaneous ligand fluorescence signal detection with single-wavelength excitation in a flow. DPG Spring Meeting 2017 Dresden, Germany 19.03.2017 24.03.2017 0 Daniel Kage eng uncontrolled Flow cytometry eng uncontrolled Fluorescence eng uncontrolled Life sciences eng uncontrolled Lifetime encoding eng uncontrolled Polymer particles Analytische Chemie Datei im Netzwerk der BAM verfügbar ("Closed Access") Präsentation