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    <title language="eng">Semisynthesis of Stable Isotope-Labeled Ergot Alkaloids for HPLC-MS/MS Analysis</title>
    <abstract language="eng">Ergot alkaloids (EAs) are prevalent food contaminants affecting cereals, such as rye, wheat, and barley worldwide. To ensure EU safety standards, the six most common EAs: ergometrine, ergotamine, ergosine, ergocornine, ergocristine, and ergocryptine, and their epimers, are quantified using HPLC-MS/MS, as described in the European Standard Method EN 17425:2021. However, this can be challenging and time-consuming in food matrices without appropriate internal standards and highlights the need for more robust and precise analytical tools to support their monitoring. The development of isotope-labeled EAs directly addresses this gap, offering improved accuracy and leading to more consistency across laboratories and consequently to more consumer safety. Therefore, we developed a semisynthetic approach, building upon our previous work where native ergotamine was N6-demethylated to norergotamine and subsequently remethylated using iodomethane (13CD3-I). Herein, we are now able to present the successful synthesis of all of the isotopically labeled priority EAs. These isotope-labeled standards were tested against their native counterparts using HPLC coupled with HR-MS/MS. The chromatographic and mass spectrometric properties of the unlabeled and isotopically labeled EAs match exactly, confirming their successful synthesis and structure. These standards can now be utilized to enhance the accuracy and reliability of EA quantification in food and feed.</abstract>
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    <author>Sven-Oliver Herter</author>
    <author>Hajo Haase</author>
    <author>Matthias Koch</author>
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      <language>eng</language>
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      <value>Mycotoxins</value>
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      <language>eng</language>
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      <value>Ergot Alkaloids</value>
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      <language>eng</language>
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      <value>Stable Isotope Dilution Analysis</value>
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      <language>eng</language>
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      <value>Semisynthesis</value>
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    <collection role="institutes" number="">1.7 Organische Spuren- und Lebensmittelanalytik</collection>
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    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
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