<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>48985</id>
    <completedYear/>
    <publishedYear>2019</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>11520</pageFirst>
    <pageLast>11528</pageLast>
    <pageNumber/>
    <edition/>
    <issue>18</issue>
    <volume>91</volume>
    <type>article</type>
    <publisherName>American Chemical Society (ACS Publications)</publisherName>
    <publisherPlace>Washington</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Arraying of single cells for quantitative high throughput laser ablation ICP-TOF-MS</title>
    <abstract language="eng">Arraying of single cells for mass spectrometric analysis is a considerable bioanalytical challenge. In this study, we employ a novel single cell arraying technology for quantitative analysis and isotopic ﬁngerprinting by laser ablation inductively coupled plasma time-of-ﬂight mass spectrometry (LA-ICP-TOF-MS). The single cell arraying approach is based on a piezo-acoustic microarrayer with software for automated optical detection of cells within the piezo dispense capillary (PDC) prior to arraying. Using optimized parameters, single cell occupancy of &gt;99%, high throughput (up to 550 cells per hour), and a high cell recovery of &gt;66% is achieved. LA-ICP-TOF-MS is employed to detect naturally occurring isotopes in the whole mass range as ﬁngerprints of individual cells. Moreover, precise quantitative determination of metal-containing cell dyes is possible down to contents of ∼100 ag using calibration standards which were produced using the same arrayer.</abstract>
    <parentTitle language="eng">Analytical Chemistry</parentTitle>
    <identifier type="doi">10.1021/acs.analchem.9b00198</identifier>
    <identifier type="issn">0003-2700</identifier>
    <identifier type="issn">1520-6882</identifier>
    <enrichment key="date_peer_review">18.09.2019</enrichment>
    <author>Konrad Löhr</author>
    <author>O. Borovinskaya</author>
    <author>G. Tourniaire</author>
    <author>Ulrich Panne</author>
    <author>Norbert Jakubowski</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Laser ablation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Cell</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Array</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ICP-MS</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
</export-example>
