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    <title language="eng">An Electrochemical Immunosensor for Pharmaceutical Contaminants in Water</title>
    <abstract language="eng">Contamination of drinking water with pharmaceuticals is an emerging problem and a potential health threat. Immunochemical methods based on the binding of the analyte to specific antibodies enable fast &amp; cost-effective on-site analyses. Magnetic bead-based immunoassays allow for implementation into an immunosensor for online testing. Particles are prepared by either coupling the analyte molecule (diclofenac) or the respective antibody (amoxicillin) to the surface. For miniaturization, detection is performed electrochemically (chronoamperometry) on a microfluidic chip. The developed immunosensor will enable detection of pharmaceuticals directly in water supply pipes.</abstract>
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    <author>Alexander Ecke</author>
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      <language>eng</language>
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      <value>Amoxicillin</value>
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      <language>eng</language>
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      <value>Diclofenac</value>
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      <language>eng</language>
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      <value>Microfluidics</value>
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      <language>eng</language>
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      <value>Chronoamperometry</value>
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    <title language="eng">An Immunosensor for Pharmaceutical Contaminants in Drinking Water</title>
    <abstract language="eng">Contamination of drinking water with pharmaceuticals is an emerging problem and a potential health threat. Immunochemical methods based on the binding of the analyte to specific antibodies enable fast &amp; cost-effective on-site analyses. Magnetic bead-based immunoassays (MBBAs) allow for implementation into an immunosensor for online testing. Particles are prepared by either coupling the analyte molecule (diclofenac) or the respective antibody (amoxicillin) to the surface. For miniaturization, detection is performed electrochemically (chronoamperometry) on a microfluidic chip. The developed immunosensor will enable detection of pharmaceuticals directly in water supply pipes.</abstract>
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    <author>Alexander Ecke</author>
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      <value>Immunosensor</value>
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    <subject>
      <language>eng</language>
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      <value>Electrochemistry</value>
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    <subject>
      <language>eng</language>
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      <value>Amoxicillin</value>
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    <subject>
      <language>eng</language>
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      <value>Diclofenac</value>
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      <language>eng</language>
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      <value>Magnetic Beads</value>
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    <title language="eng">An Immunosensor for Pharmaceutical Contaminants in Water Supply</title>
    <abstract language="eng">Contamination of drinking water with pharmaceuticals is an emerging problem and a potential health threat. Immunochemical methods based on the binding of the analyte to specific antibodies enable fast and cost-effective on-site analyses. Magnetic bead-based immunoassays allow for implementation into an immunosensor for online testing. These particles are prepared by either coupling the analyte molecule (diclofenac) or the respective antibody (amoxicillin) to the surface. For miniaturization, detection is performed electrochemically on a microfluidic chip. The developed immunosensor will enable detection of pharmaceuticals directly in water supply pipes.</abstract>
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    <author>Alexander Ecke</author>
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      <value>Immunosensor</value>
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      <language>eng</language>
      <type>uncontrolled</type>
      <value>Magnetic Beads</value>
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      <language>eng</language>
      <type>uncontrolled</type>
      <value>Diclofenac</value>
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      <language>eng</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
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      <language>eng</language>
      <type>uncontrolled</type>
      <value>Water Analysis</value>
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    <title language="eng">An Immunosensor for Pharmaceutical Contaminants in Water Supply</title>
    <abstract language="eng">Contamination of drinking water with pharmaceuticals is an emerging problem and a potential health threat. Immunochemical methods based on the binding of the analyte to specific antibodies enable fast &amp; cost-effective on-site analyses. Magnetic bead-based immunoassays (MBBAs) allow for implementation into an immunosensor for online testing. Particles are prepared by either coupling the analyte molecule (diclofenac) or the respective antibody (amoxicillin) to the surface. For miniaturization, detection is performed electrochemically (chronoamperometry) on a microfluidic chip. The developed immunosensor will enable detection of pharmaceuticals directly in water supply pipes.</abstract>
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    <author>Alexander Ecke</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Immunosensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Magnetic Particles</value>
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      <language>eng</language>
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      <value>Microfluidics</value>
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      <language>eng</language>
      <type>uncontrolled</type>
      <value>Amperometry</value>
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    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Diclofenac</value>
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    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
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    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
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  <doc>
    <id>51061</id>
    <completedYear/>
    <publishedYear>2020</publishedYear>
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    <language>deu</language>
    <pageFirst>466</pageFirst>
    <pageLast>470</pageLast>
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    <title language="deu">Immunanalytische Bestimmung von β-Lactam-Antibiotika und deren Hydrolyseprodukten im Trinkwasser</title>
    <abstract language="deu">Die speziellen Herausforderungen der immunanalytischen Bestimmung von β-Lactam-Antibiotika in (Trink-)Wasserproben werden anhand eines neu entwickelten ELISA (enzyme-linked immunosorbent assay) für das Breitbandantibiotikum Amoxicillin diskutiert. Es konnte gezeigt werden, dass die Hydrolyse des Analyten einen positiven Einfluss auf die Erkennung durch den Antikörper hat. Das entsprechende Hydrolyseprodukt, zu dem der Antikörper folglich eine höhere Affinität aufweist, wurde identifiziert und ein entsprechendes Verhalten wurde auch für andere β-Lactam-Antibiotika aus der Gruppe der Penicilline beobachtet. Eine Strategie für die Analyse von Wasserproben hinsichtlich Kontamination mit den hier betrachteten β-Lactamen und deren Hydrolyseprodukten wurde ausgearbeitet.</abstract>
    <parentTitle language="deu">Wasser 2020 - Kurzreferate zur Wasser 2020</parentTitle>
    <identifier type="url">https://www.wasserchemische-gesellschaft.de/images/pdf_veranstaltungen/2020/Wasserchemische_Gesellschaft_Tagungsband_Wasser_2020_s.pdf</identifier>
    <enrichment key="eventName">Wasser 2020 - Jahrestagung der Wasserchemischen Gesellschaft</enrichment>
    <enrichment key="eventPlace">Meeting was canceled</enrichment>
    <enrichment key="eventStart">18.05.2020</enrichment>
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    <author>Alexander Ecke</author>
    <author>Rudolf Schneider</author>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>ELISA</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Antibiotika</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Hydrolyseprodukte</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
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    <id>59190</id>
    <completedYear/>
    <publishedYear>2023</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>129</pageLast>
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    <edition/>
    <issue/>
    <volume/>
    <type>doctoralthesis</type>
    <publisherName/>
    <publisherPlace>Berlin</publisherPlace>
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    <thesisDateAccepted>2023-02-21</thesisDateAccepted>
    <title language="eng">Integrated Diagnostics of Pharmaceutical Contaminants in Water Supply and Management Systems</title>
    <abstract language="eng">The contamination of drinking water with pharmaceuticals represents a severe health risk. In order to monitor the drinking water quality continuously and enable quick countermeasures in case of contamination, novel sensors are required. Here, immunoanalytical methods based on the binding of the analyte to highly selective antibodies can be helpful. In this work, magnetic bead-based immunoassays (MBBAs) have been developed for the detection of two relevant contaminants of drinking water: diclofenac (DCF) and amoxicillin (AMX). In case of the latter, not only the parent drug is of interest in the risk assessment but also its hydrolysis products (HPs). In a comprehensive study, the influence of external factors and intrinsic properties of the water on the rate of hydrolysis was investigated. As the hydrolysis of AMX further impacts the recognition by the antibody, a strategy to analyze samples with unknown hydrolysis degree of AMX was established employing the enzyme β-lactamase in sample preparation. For both analytes, the MBBAs enable the fast quantification with results obtained in less than one hour which represents a major improvement over conventional immunoassays like the enzyme-linked immunosorbent assay (ELISA). Compared to the respective ELISAs with the same antibodies, the MBBAs further exhibit improved analytical parameters such as a broader measurement range and lower limits of detection. Due to the magnetic properties of the beads that serve as a platform for the assays, they are suitable for the mobile and automated detection at the point-of-care. An integrated diagnostic system was designed in which electrochemical detection with chronoamperometry on a microfluidic chip allows for further miniaturization of the system to enable monitoring of the drinking water quality online in water supply pipes at waterworks.</abstract>
    <abstract language="deu">Die Kontamination von Trinkwasser mit Arzneimitteln stellt eine ernste Gesundheitsgefahr dar. Um die Trinkwasserqualität kontinuierlich überwachen und im Falle einer Verunreinigung zeitnah reagieren zu können, sind neuartige Sensoren erforderlich. Hier können immunanalytische Methoden, die auf der Bindung des Analyten an hochselektive Antikörper beruhen, hilfreich sein. In dieser Arbeit wurden magnetpartikelbasierte Immunoassays (MBBAs) für zwei relevante Kontaminanten des Trinkwassers entwickelt: Diclofenac (DCF) und Amoxicillin (AMX). Bei letzterem erwiesen sich neben der Ausgangsverbindung auch dessen Hydrolyseprodukte (HPs) als relevant für die Gefährdungsbeurteilung. In einer umfassenden Studie wurde der Einfluss von externen Faktoren und intrinsischen Eigenschaften des Wassers auf die Hydrolysegeschwindigkeit untersucht. Da die Hydrolyse von AMX auch die Erkennung durch den Antikörper beeinflusst, wurde eine Strategie zur Analyse von Proben mit unbekanntem Hydrolysegrad von AMX unter Verwendung des Enzyms β-Lactamase in der Probenvorbereitung entwickelt. Für beide Analyten ermöglichen die MBBAs eine schnelle Quantifizierung mit Ergebnissen in weniger als einer Stunde, was eine wesentliche Verbesserung gegenüber herkömmlichen Immunoassays wie dem Enzyme-linked Immunosorbent Assay (ELISA) darstellt. Im Vergleich zu den entsprechenden ELISAs mit denselben Antikörpern weisen die MBBAs zudem verbesserte analytische Parameter auf, wie einen breiteren Messbereich und niedrigere Nachweisgrenzen. Aufgrund der magnetischen Eigenschaften der Partikel, die als Plattform für die Assays dienen, eignen sie sich für den mobilen und automatisierten Einsatz vor Ort. Ein integriertes Diagnosesystem, bei dem die elektrochemische Detektion mittels Chronoamperometrie auf einem mikrofluidischen Chip eine weitere Miniaturisierung des Systems ermöglicht, wurde entworfen, um die Überwachung der Trinkwasserqualität online in Wasserwerken zu ermöglichen.</abstract>
    <identifier type="doi">10.18452/25853</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-591901</identifier>
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    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>Alexander Ecke</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Antibiotics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Diclofenac</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biosensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Antibodies</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
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    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <thesisGrantor>Humboldt-Universität zu Berlin</thesisGrantor>
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    <id>52426</id>
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    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
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    <title language="eng">Magnetic Bead-Based Immunoassays for Online Drinking Water Analysis</title>
    <abstract language="eng">We present the development of magnetic bead-based immunoassays for two potential contaminants of drinking water: the nonsteroidal anti-inflammatory drug diclofenac and the broad-spectrum antibiotic amoxicillin. For these analytes, different assay formats were developed which can generally be transferred to other analytes. The use of magnetic beads not only enables implementation of the assays into an online system since the beads can be reversibly immobilized by applying a magnetic field. Furthermore, our MBBAs hold several advantages over the conventional ELISAs (enzyme-linked immunosorbent assays) for both analytes, such as lower limits of quantification, shorter assay duration and less washing steps required.&#13;
Strategies for the setup and miniaturization of an online  immunosensor including electrochemical readout on a microfluidic chip will be discussed.</abstract>
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    <author>Alexander Ecke</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Magnetic Beads</value>
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    <subject>
      <language>eng</language>
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      <value>Immunoassay</value>
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    <subject>
      <language>eng</language>
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      <language>eng</language>
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      <language>eng</language>
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      <value>Amoxicillin</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
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    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
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  </doc>
  <doc>
    <id>52285</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
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    <volume/>
    <type>poster</type>
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    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Magnetic Bead-Based Immunoassays for Online Sensing Applications</title>
    <abstract language="eng">The demand for quick and reliable online sensors is rising not only in the field of environmental analysis with new and diversifying sources of pollution. A major aspect of this is the contamination of (drinking) water with pharmaceuticals representing a potential health threat and requiring timely countermeasures in case of contamination.&#13;
In this regard, antibody-based methods can be helpful as they enable fast and cost-effective on-site and potentially online analyses. The transition from the conventional plate-based formats like ELISA (enzyme-linked immunosorbent assay) to an immunosensor fit for online sensing can be achieved by using magnetic microparticles as a mobile sensing platform.&#13;
Beads are prepared by either coupling the antibody or the analyte to the surface. Both these strategies are presented using the example of two relevant contaminants in water: diclofenac (DCF) &amp; amoxicillin (AMX), and can be transferred to other analytes.&#13;
The developed magnetic bead-based assays (MBBAs) exhibit lower LODs, enhanced sensitivity as well as shorter time of analysis than the respective ELISAs and can be integrated into an immunosensor with electrochemical detection on a microfluidic chip.</abstract>
    <enrichment key="eventName">European Biosensor Symposium 2021</enrichment>
    <enrichment key="eventPlace">Online meeting</enrichment>
    <enrichment key="eventStart">09.03.2021</enrichment>
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    <enrichment key="opus.doi.autoCreate">false</enrichment>
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    <author>Alexander Ecke</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Magnetic Beads</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Diclofenac</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Online Sensing</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="literaturgattung" number="">Präsentation</collection>
  </doc>
  <doc>
    <id>52736</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>deu</language>
    <pageFirst>48</pageFirst>
    <pageLast>51</pageLast>
    <pageNumber/>
    <edition/>
    <issue>2</issue>
    <volume>27</volume>
    <type>article</type>
    <publisherName>Gesellschaft Deutscher Chemiker (GDCh)</publisherName>
    <publisherPlace>Frankfurt/Main</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="deu">Magnetpartikelbasierte Immunoassays als vielseitiges Werkzeug für die Umweltanalytik</title>
    <abstract language="deu">Der Bedarf nach schnellen und kostengünstigen Analysemethoden in der (Trink-)Wasseranalytik steigt mit dem zunehmenden Eintrag pharmazeutisch aktiver Substanzen in die Umwelt.&#13;
Insbesondere die Kontamination mit Antibiotika erscheint neben ökotoxikologischen Effekten vor allem in Hinblick auf die Evolution resistenter Keime im Wasser besorgniserregend. &#13;
Wir berichten hier über die Entwicklung eines magnetpartikelbasierten Immunoassays (MBBA) zur Detektion des Breitbandantibiotikums Amoxicillin (AMX) in Wasserproben, der im Vergleich zum entsprechenden Enzyme-linked Immunosorbent Assay (ELISA) eine wesentlich kürzere Analysendauer sowie einen breiteren Messbereich mit niedrigerer Nachweisgrenze aufweist. Das angewendete Assayprinzip ist dabei leicht auf weitere Analyten übertragbar und ermöglicht die Implementierung in einen Immunosensor.</abstract>
    <parentTitle language="deu">Mitteilungen der Fachgruppe Umweltchemie und Ökotoxikologie</parentTitle>
    <identifier type="issn">1618-3258</identifier>
    <identifier type="url">https://www.gdch.de/netzwerk-strukturen/fachstrukturen/umweltchemie-und-oekotoxikologie/publikationen/mitteilungen/archiv/2021.html</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-527366</identifier>
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    <licence>Creative Commons - CC BY-NC - Namensnennung - Nicht kommerziell 4.0 International</licence>
    <author>Alexander Ecke</author>
    <author>Konrad Bohm</author>
    <author>Rudolf Schneider</author>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Magnetpartikel</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Wasser</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Antikörper</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="literaturgattung" number="">Graue Literatur</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/52736/Ecke_et_al_Mitt_Umweltchem_Oekotox_02_2021.pdf</file>
  </doc>
  <doc>
    <id>51345</id>
    <completedYear/>
    <publishedYear>2020</publishedYear>
    <thesisYearAccepted/>
    <language>mul</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>lecture</type>
    <publisherName/>
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    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="mul">Magnetpartikelbasierte Immunoassays für die Online-Trinkwasseranalyik</title>
    <abstract language="mul">Wir berichten hier über die Entwicklung plattenbasierter Magnetpartikel-Immunoassays für die Bestimmung des Breitbandantibiotikums Amoxicillin und des nicht-steroidalen Antirheumatikums Diclofenac im Trinkwasser. Für die beiden Analyten wurden unterschiedliche Ansätze entwickelt, mit partikelgebundenen Antikörpern für AMX und Analyt-gekoppelten Partikeln für DCF, die die simultane Detektion beider Kontaminanten im nanomolaren Konzentrationsbereich erlauben.</abstract>
    <enrichment key="eventName">Interdisziplinäres Doktorandenseminar des AK Prozessanalytik</enrichment>
    <enrichment key="eventPlace">Online meeting</enrichment>
    <enrichment key="eventStart">21.09.2020</enrichment>
    <enrichment key="eventEnd">22.09.2020</enrichment>
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    <author>Alexander Ecke</author>
    <subject>
      <language>mul</language>
      <type>uncontrolled</type>
      <value>Magnetpartikel</value>
    </subject>
    <subject>
      <language>mul</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <subject>
      <language>mul</language>
      <type>uncontrolled</type>
      <value>Trinkwasseranalytik</value>
    </subject>
    <subject>
      <language>mul</language>
      <type>uncontrolled</type>
      <value>Diclofenac</value>
    </subject>
    <subject>
      <language>mul</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="literaturgattung" number="">Präsentation</collection>
  </doc>
  <doc>
    <id>52640</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>deu</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>poster</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="deu">Magnetpartikelbasierte Immunoassays für die Online-Trinkwasseranalytik</title>
    <abstract language="deu">Die zunehmende Kontamination von (Trink)-Wasser mit pharmazeutischen Rückständen stellt ein potenzielles Gesundheitsrisiko dar und verlangt nach schnellen und zuverlässigen Analysemethoden. Hierbei können antikörper-basierte Methoden helfen, da sie schnelle und kostengünstige Vor-Ort-Messungen sowie Onlineanalysen ermöglichen. &#13;
Der Übergang von konventionellen plattenbasierten Formaten, wie ELISA (Enzyme-linked Immunosorbent Assay), zu einem Immunosensor für Online-Messungen wird durch den Einsatz magnetischer Mikropartikel als mobile Messplattform ermöglicht.&#13;
Die Partikel werden dazu entweder mit dem Antikörper oder dem Analyten gekoppelt. Diese beiden Strategien werden am Beispiel zweier relevanter Kontaminanten des Wassers gezeigt: Diclofenac (DCF) &amp; Amoxicillin (AMX), und sind leicht auf andere Analyten übertragbar. Die magnetpartikelbasierten Assays (MBBAs) weisen niedrigere Nachweisgrenzen, breitere Messbereiche sowie kürzere Analysezeiten als die entsprechenden ELISAs auf und können in einen Immunosensor mit elektrochemischer Detektion auf einem mikrofluidischen Chip integriert werden.</abstract>
    <enrichment key="eventName">Wasser 2021</enrichment>
    <enrichment key="eventPlace">Online meeting</enrichment>
    <enrichment key="eventStart">10.05.2021</enrichment>
    <enrichment key="eventEnd">12.05.2021</enrichment>
    <enrichment key="opus.source">publish</enrichment>
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    <author>Alexander Ecke</author>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Immunosensor</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Magnetpartikel</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Diclofenac</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
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  </doc>
  <doc>
    <id>52283</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>298</pageFirst>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>10</volume>
    <type>article</type>
    <publisherName>MDPI</publisherName>
    <publisherPlace>Basel, CH</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Pitfalls in the Immunochemical Determination of β-Lactam Antibiotics in Water</title>
    <abstract language="eng">Contamination of waters with pharmaceuticals is an alarming problem as it may support the evolution of antimicrobial resistance. Therefore, fast and cost-effective analytical methods for potential on-site analysis are desired in order to control the water quality and assure the safety of its use as a source of drinking water. Antibody-based methods, such as the enzyme-linked immunosorbent assay (ELISA), can be helpful in this regard but can also have certain pitfalls in store, depending on the analyte. As shown here for the class of β-lactam antibiotics, hydrolysis of the β-lactam ring is a key factor in the immunochemical analysis as it influences antibody recognition. With the antibody used in this study, the limit of detection (LOD) in the immunoassay could be significantly reduced by hydrolysis for the five tested penicillins, with the lowest LOD for carbenicillin (0.2 nmol/L) and the greatest impact on penicillins G and V (reduction by 85%). In addition to enhanced quantification, our strategy also provides access to information about the degree of hydrolysis in water samples as shown for the most abundant penicillin amoxicillin.</abstract>
    <parentTitle language="eng">Antibiotics</parentTitle>
    <identifier type="issn">2079-6382</identifier>
    <identifier type="doi">10.3390/antibiotics10030298</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-522832</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">12.04.2021</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Alexander Ecke</author>
    <author>Rudolf Schneider</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ELISA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>β-Lactam</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Hydrolysis</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/52283/Ecke_Pitfalls Immunochemical Determination of beta-Lactams in Water.pdf</file>
  </doc>
  <doc>
    <id>56026</id>
    <completedYear/>
    <publishedYear>2022</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>poster</type>
    <publisherName/>
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    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">The Fate of the Antibiotic Amoxicillin in the Aquatic Environment</title>
    <abstract language="eng">Contamination of the environment with antibiotics is of great concern as it promotes the evolution of antimicrobial resistances. In case of amoxicillin (AMX) in the aquatic environment, further risk arises from hydrolysis products (HPs) which can cause allergy. To assess these risks, a comprehensive investigation and understanding of the degradation of AMX is necessary. We investigated the hydrolysis rate of AMX in different types of water as well as the influence of temperature and irradiation. The content of the heavy metal ions copper and zinc was found to be crucial for the hydrolysis rate of AMX and stability of HPs. Eventually, a new degradation pathway for AMX could be elaborated and confirmed by tandem mass spectrometry (LC-MS/MS).</abstract>
    <enrichment key="eventName">Berliner Chemie in Praxis Symposium</enrichment>
    <enrichment key="eventPlace">Berlin, Germany</enrichment>
    <enrichment key="eventStart">07.10.2022</enrichment>
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    <author>Alexander Ecke</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Hydrolysis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Amoxicillin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>LC-MS/MS</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ICP-MS</value>
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    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
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    <collection role="institutes" number="">1.1 Anorganische Spurenanalytik</collection>
    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
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    <collection role="themenfelder" number="">Umwelt-Material-Interaktionen</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="literaturgattung" number="">Präsentation</collection>
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