<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>32847</id>
    <completedYear/>
    <publishedYear>2015</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>244</pageFirst>
    <pageLast>253</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>87</volume>
    <type>article</type>
    <publisherName>Wiley-Liss</publisherName>
    <publisherPlace>Hoboken, NJ</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Quantification of cells with specific phenotypes I: Determination of CD4+ cell count per microliter in reconstituted lyophilized human PBMC prelabeled with anti-CD4 FICT antibody</title>
    <abstract language="eng">A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells prelabeled with a fluorescein isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL preparation is intended to be used as a reference material for CD4+ cell counting including the development of higher order reference measurement procedures and has been evaluated in the pilot study CCQM-P102. This study was conducted across 16 laboratories from eight countries to assess the ability of participants to quantify the CD4+ cell count of this reference material and to document cross-laboratory variability plus associated measurement uncertainties. Twelve different flow cytometer platforms were evaluated using a standard protocol that included calibration beads used to obtain quantitative measurements of CD4+ T cell counts. There was good overall cross-platform and counting method agreement with a grand mean of the laboratory calculated means of (301.7 ± 4.9) µL-1 CD4+ cells. Excluding outliers, greater than 90% of participant data agreed within ±15%. A major contribution to variation of sLL CD4+ cell counts was tube to tube variation of the calibration beads, amounting to an uncertainty of 3.6%. Variation due to preparative steps equated to an uncertainty of 2.6%. There was no reduction in variability when data files were centrally reanalyzed. Remaining variation was attributed to instrument specific differences. CD4+ cell counts obtained in CCQM-P102 are in excellent agreement and show the robustness of both the measurements and the data analysis and hence the suitability of sLL as a reference material for interlaboratory comparisons and external quality assessment.</abstract>
    <parentTitle language="eng">Cytometry / A</parentTitle>
    <identifier type="old">35923</identifier>
    <identifier type="doi">10.1002/cyto.a.22614</identifier>
    <identifier type="issn">0196-4763</identifier>
    <identifier type="issn">1552-4922</identifier>
    <identifier type="issn">1552-4930</identifier>
    <author>R. Stebbings</author>
    <author>L. Wang</author>
    <author>J. Sutherland</author>
    <author>M. Kammel</author>
    <author>A.K. Gaigalas</author>
    <author>M. John</author>
    <author>B. Roemer</author>
    <author>Maren Kuhne</author>
    <author>Rudolf Schneider</author>
    <author>M. Braun</author>
    <author>A. Engel</author>
    <author>D.K. Dikshit</author>
    <author>F. Abbasi</author>
    <author>G.E. Marti</author>
    <author>M.P. Sassi</author>
    <author>L. Revel</author>
    <author>S.-K. Kim</author>
    <author>M.-O. Baradez</author>
    <author>T. Lekishvili</author>
    <author>D. Marshall</author>
    <author>L. Whitby</author>
    <author>W. Jing</author>
    <author>V. Ost</author>
    <author>M. Vonsky</author>
    <author>J. Neukammer</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>CD4+ cell counting</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Relative concentration measurement</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lyophilized cells</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Flow cytometry</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Standard measurement procedure</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Measurement of uncertainty</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Human immunodeficiency virus-1</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Acquired immunodeficiency syndrome</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference material</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
</export-example>
