<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>22688</id>
    <completedYear/>
    <publishedYear>2010</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>8076</pageFirst>
    <pageLast>8083</pageLast>
    <pageNumber/>
    <edition/>
    <issue>24</issue>
    <volume>76</volume>
    <type>article</type>
    <publisherName>American Society for Microbiology</publisherName>
    <publisherPlace>Washington, DC [u.a.]</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Biodegradation of a biocide (Cu-N-cyclohexyldiazenium dioxide) component of a wood preservative by a defined soil bacterial community</title>
    <abstract language="eng">The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, the fate of isotope-labeled (13C) and nonlabeled (12C) Cu-HDO incorporated in wood sawdust mixed with soil was investigated. HDO concentration was monitored by high-pressure liquid chromatography. The total carbon and the δ13C content of respired CO2, as well as of the soil-wood-sawdust mixture, were determined with an elemental analyzer-isotopic ratio mass spectrometer. The concentration of HDO decreased significantly after 105 days of incubation, and after 24 days the 13CO2 concentration respired from soil increased steadily to a maximum after 64 days of incubation. Phospholipid fatty acid-stable isotope probing (PFA-SIP) analysis revealed that the dominant PFAs C19:0d8,9, C18:0, C18:1ω7, C18:2ω6,9, C17:1d7,8, C16:0, and C16:1ω7 were highly enriched in their δ13C content. Moreover, RNA-SIP identified members of the phylum Acidobacteria and the genera Phenylobacterium and Comamonas that were assimilating carbon from HDO exclusively. Cu-HDO as part of a wood preservative effectively decreased fungal wood decay and overall microbial respiration from soil. In turn, a defined bacterial community was stimulated that was able to metabolize HDO completely.</abstract>
    <parentTitle language="eng">Applied and environmental microbiology</parentTitle>
    <identifier type="old">25236</identifier>
    <identifier type="doi">10.1128/AEM.01092-10</identifier>
    <identifier type="issn">0099-2240</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">09.12.2010</enrichment>
    <author>Désirée Jakobs</author>
    <author>Helena Mathies</author>
    <author>W.-R. Abraham</author>
    <author>Wolfgang Pritzkow</author>
    <author>Ina Stephan</author>
    <author>Matthias Noll</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>16S rRNA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biocide</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>PLFA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Stable isotope probing</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Soil</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Wood preservative</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
</export-example>
