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  <doc>
    <id>53062</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>56</pageFirst>
    <pageLast>73</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>284</volume>
    <type>article</type>
    <publisherName>Wiley-Blackwell</publisherName>
    <publisherPlace>Oxford</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy</title>
    <abstract language="eng">A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated, quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing and quantifying scientific phenomena, neither a thorough understanding of the principles underlying quantitative imaging techniques nor appropriate knowledge of how to calibrate, operate and maintain microscopes can be taken for granted. This is clearly demonstrated by the well-documented and widespread difficulties that are routinely encountered in evaluating acquired data and reproducing scientific experiments. Indeed, studies have shown that more than 70% of researchers have tried and failed to repeat another scientist’s experiments, while more than half have even failed to reproduce their own experiments1. One factor behind the reproducibility crisis of experiments published in scientific journals is the frequent underreporting of imaging methods caused by a lack of awareness and/or a lack of knowledge of the applied technique2,3. Whereas quality control procedures for some methods used in biomedical research, such as genomics (e.g., DNA sequencing, RNA-seq) or cytometry, have been introduced (e.g. ENCODE4), this issue has not been tackled for optical microscopy instrumentation and images. Although many calibration standards and protocols have been published, there is a lack of awareness and agreement on common Standards and guidelines for quality assessment and reproducibility5.&#13;
In April 2020, the QUality Assessment and REProducibility for instruments and images in Light Microscopy (QUAREP-LiMi) initiative6 was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models7,8, and tools9,10, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research.&#13;
This White Paper 1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; 2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of Stakeholders including, researchers, imaging scientists11, bioimage analysts, bioimage informatics developers, corporate partners, Funding agencies, standards organizations, scientific publishers, and observers of such; 3) outlines the current actions of the QUAREPLiMi initiative, and 4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC.&#13;
To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.</abstract>
    <parentTitle language="eng">Journal of microscopy</parentTitle>
    <identifier type="doi">10.1111/jmi.13041</identifier>
    <identifier type="issn">1365-2818</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-530629</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">05.08.2021</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>G. Nelson</author>
    <author>U. Boehm</author>
    <author>S. Bagley</author>
    <author>P. Bajcsy</author>
    <author>J. Bischof</author>
    <author>C. M. Brown</author>
    <author>A. Dauphin</author>
    <author>I. M. Dobbie</author>
    <author>J. E. Eriksson</author>
    <author>O. Faklaris</author>
    <author>J. Fernandez-Rodriguez</author>
    <author>A. Ferrand</author>
    <author>L, Gelman</author>
    <author>A. Gheisari</author>
    <author>H. Hartmann</author>
    <author>C. Kukat</author>
    <author>A. Laude</author>
    <author>M. Mitkovski</author>
    <author>S. Munck</author>
    <author>A. J. North</author>
    <author>T. Rasse</author>
    <author>Ute Resch-Genger</author>
    <author>L. C. Schuetz</author>
    <author>A. Seitz</author>
    <author>C. Strambio-De-Castillia</author>
    <author>J. R. Swedlow</author>
    <author>I. Alexopoulos</author>
    <author>K. Aumayr</author>
    <author>S. Avilov</author>
    <author>G.-J. Bakker</author>
    <author>R. R. Bammann</author>
    <author>A. Bassi</author>
    <author>H. Beckert</author>
    <author>S. Beer</author>
    <author>Y. Belyaev</author>
    <author>J. Bierwagen</author>
    <author>K. A. Birngruber</author>
    <author>M. Bosch</author>
    <author>J. Breitlow</author>
    <author>L. A. Cameron</author>
    <author>J. Chalfoun</author>
    <author>J. J. Chambers</author>
    <author>C.-L. Chen</author>
    <author>E. Conde-Sousa</author>
    <author>A. D. Corbett</author>
    <author>F. P. Cordelieres</author>
    <author>E. Del Nery</author>
    <author>R. Dietzel</author>
    <author>F. Eismann</author>
    <author>E. Fazeli</author>
    <author>A. Felscher</author>
    <author>H. Fried</author>
    <author>N. Gaudreault</author>
    <author>W. I. Goh</author>
    <author>T. Guilbert</author>
    <author>R. Hadleigh</author>
    <author>P. Hemmerich</author>
    <author>G. A. Holst</author>
    <author>M. S. Itano</author>
    <author>C. B. Jaffe</author>
    <author>H. K. Jambor</author>
    <author>S. C. Jarvis</author>
    <author>A. Keppler</author>
    <author>D. Kirchenbuechler</author>
    <author>M. Kirchner</author>
    <author>N. Kobayashi</author>
    <author>G. Krens</author>
    <author>S. Kunis</author>
    <author>J. Lacoste</author>
    <author>M. Marcello</author>
    <author>G. G. Martins</author>
    <author>D. J. Metcalf</author>
    <author>C. A. Mitchell</author>
    <author>J. Moore</author>
    <author>T. Mueller</author>
    <author>M. S. Nelson</author>
    <author>S. Ogg</author>
    <author>S. Onami</author>
    <author>A. L. Palmer</author>
    <author>P. Paul-Gilloteaux</author>
    <author>J. A. Pimentel</author>
    <author>L. Plantard</author>
    <author>S. Podder</author>
    <author>E. Rexhepaj</author>
    <author>A. Royon</author>
    <author>M. A. Saari</author>
    <author>D. Schapman</author>
    <author>V. Schoonderwoert</author>
    <author>B. Schroth-Diez</author>
    <author>S. Schwartz</author>
    <author>M. Shaw</author>
    <author>M. Spitaler</author>
    <author>M. T. Stoeckl</author>
    <author>D. Sudar</author>
    <author>J. Teillon</author>
    <author>S. Terjung</author>
    <author>R. Thuenauer</author>
    <author>C. D. Wilms</author>
    <author>G. D. Wright</author>
    <author>R. Nitschke</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fluorescence</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Microscopy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Quality assurance</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Comparability</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Standards</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference materials</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reliability</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Data</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference data</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biology</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Medicine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Life science</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.2 Biophotonik</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/53062/jmi.13041.pdf</file>
  </doc>
  <doc>
    <id>54691</id>
    <completedYear/>
    <publishedYear>2022</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>677</pageFirst>
    <pageLast>688</pageLast>
    <pageNumber/>
    <edition/>
    <issue>10</issue>
    <volume>57</volume>
    <type>article</type>
    <publisherName>Wolters Kluwer N.V.</publisherName>
    <publisherPlace>Alphen aan den Rijn, The Netherlands</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Different Impact of Gadopentetate and Gadobutrol on Inflammation-Promoted Retention and Toxicity of Gadolinium Within the Mouse Brain</title>
    <abstract language="eng">Objectives: Using a murine model of multiple sclerosis, we previously showed that repeated administration of gadopentetate dimeglumine led to retention of gadolinium (Gd) within cerebellar structures and that this process was enhanced with inflammation. This study aimed to compare the kinetics and retention profiles of Gd in inflamed and healthy brains after application of the macrocyclic Gd-based contrast agent (GBCA) gadobutrol or the linear GBCA gadopentetate. Moreover, potential Gd-induced neurotoxicity was investigated in living hippocampal slices ex vivo.&#13;
Materials and Methods: Mice at peak of experimental autoimmune encephalomyelitis (EAE; n = 29) and healthy control mice (HC; n = 24) were exposed to a cumulative dose of 20 mmol/kg bodyweight of either gadopentetate dimeglumine or gadobutrol (8 injections of 2.5 mmol/kg over 10 days). Magnetic resonance imaging (7 T) was performed at baseline as well as at day 1, 10, and 40 post final injection (pfi) of GBCAs. Mice were sacrificed after magnetic resonance imaging and brain and blood Gd content was assessed by laser ablation-inductively coupled plasma (ICP)-mass spectrometry (MS) and ICP-MS, respectively. In addition, using chronic organotypic hippocampal slice cultures, Gd-induced neurotoxicity was addressed in living brain tissue ex vivo, both under control or inflammatory (tumor necrosis factor α [TNF-α] at 50 ng/μL) conditions.&#13;
Results: Neuroinflammation promoted a significant decrease in T1 relaxation times after multiple injections of both GBCAs as shown by quantitative T1 mapping of EAE brains compared with HC. This corresponded to higher Gd retention within the EAE brains at 1, 10, and 40 days pfi as determined by laser ablation-ICP-MS. In inflamed cerebellum, in particular in the deep cerebellar nuclei (CN), elevated Gd retention was observed until day 40 after last gadopentetate application (CN: EAE vs HC, 55.06 ± 0.16 μM vs 30.44 ± 4.43 μM). In contrast, gadobutrol application led to a rather diffuse Gd content in the inflamed brains, which strongly diminished until day 40 (CN: EAE vs HC, 0.38 ± 0.08 μM vs 0.17 ± 0.03 μM). The analysis of cytotoxic effects of both GBCAs using living brain tissue revealed an elevated cell death rate after incubation with gadopentetate but not gadobutrol at 50 mM. The cytotoxic effect due to gadopentetate increased in the presence of the inflammatory mediator TNF-α (with vs without TNF-α, 3.15% ± 1.18% vs 2.17% ± 1.14%; P = 0.0345).&#13;
Conclusions: In the EAE model, neuroinflammation promoted increased Gd retention in the brain for both GBCAs. Whereas in the inflamed brains, efficient clearance of macrocyclic gadobutrol during the investigated time period was observed, the Gd retention after application of linear gadopentetate persisted over the entire observational period. Gadopentetate but not gadubutrol appeared to be neurotoxic in an ex vivo paradigm of neuronal inflammation.</abstract>
    <parentTitle language="eng">Investigative Radiology</parentTitle>
    <identifier type="issn">0020-9996/22/0000–0000</identifier>
    <identifier type="doi">10.1097/RLI.0000000000000884</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-546910</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">02.05.2022</enrichment>
    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>L. Anderhalten</author>
    <author>R. V. Silva</author>
    <author>A. Morr</author>
    <author>S. Wang</author>
    <author>A. Smorodchenko</author>
    <author>Jessica Saatz</author>
    <author>Heike Traub</author>
    <author>S. Mueller</author>
    <author>P. Boehm-Sturm</author>
    <author>Y. Rodriguez-Sillke</author>
    <author>D. Kunkel</author>
    <author>J. Hahndorf</author>
    <author>F. Paul</author>
    <author>M. Taupitz</author>
    <author>I. Sack</author>
    <author>C. Infante-Duarte</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ICP-MS</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Gadolinium</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Contrast agent</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Laser ablation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Brain</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Multiple sclerosis</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.1 Anorganische Spurenanalytik</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/54691/Anderhalten_etal_InvestigativeRadiology_2022.pdf</file>
    <file>https://opus4.kobv.de/opus4-bam/files/54691/Anderhalten_etal_InvestigativeRadiology_2022_ESI.pdf</file>
  </doc>
  <doc>
    <id>53559</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>5</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>11772</volume>
    <type>article</type>
    <publisherName>SPIE</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A monolithically integrated microfluidic channel in a silicon-based photonic-integrated-circuit technology for biochemical sensing</title>
    <abstract language="eng">In this work, a cost-effective optofluidic system is proposed and preliminary experimental results are presented. A microfluidic channel monolithically integrated into a photonic integrated circuit technology is used in conjunction with a cyclic olefin copolymer (COC) substrate to provide fluidic in- and output ports. We report on initial experimental results as well as on the simple and cost-effective fabrication of this optofluidic system by means of micro-milling.</abstract>
    <parentTitle language="eng">Proceedings of SPIE</parentTitle>
    <identifier type="doi">10.1117/12.2588791</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">20.10.2021</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <author>Patrick Steglich</author>
    <author>Martin Paul</author>
    <author>C. Mai</author>
    <author>A. Böhme</author>
    <author>S. Bondarenko</author>
    <author>Michael G. Weller</author>
    <author>A. Mai</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biosensors</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biophotonics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Optical sensors</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Photonic sensors</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ring resonators</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Silicon photonics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lab-on-a-chip</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Microfluidics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Chip</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biochip</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="624">Ingenieurbau</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.5 Proteinanalytik</collection>
    <collection role="themenfelder" number="">Infrastruktur</collection>
    <collection role="themenfelder" number="">Security</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="themenfelder" number="">Sensorik</collection>
  </doc>
  <doc>
    <id>57264</id>
    <completedYear/>
    <publishedYear>2023</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>24</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>15</volume>
    <type>article</type>
    <publisherName>MDPI</publisherName>
    <publisherPlace>Basel, Schweiz</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Efficient Purification of Cowpea Chlorotic Mottle Virus by a Novel Peptide Aptamer</title>
    <abstract language="eng">The cowpea chlorotic mottle virus (CCMV) is a plant virus explored as a nanotechnological platform. The robust self-assembly mechanism of its capsid protein allows for drug encapsulation and targeted delivery. Additionally, the capsid nanoparticle can be used as a programmable platform to display different molecular moieties. In view of future applications, efficient production and purification of plant viruses are key steps. In established protocols, the need for ultracentrifugation is a significant limitation due to cost, difficult scalability, and safety issues. In addition, the purity of the final virus isolate often remains unclear. Here, an advanced protocol for the purification of the CCMV from infected plant tissue was developed, focusing on efficiency, economy, and final purity. The protocol involves precipitation with PEG 8000, followed by affinity extraction using a novel peptide aptamer. The efficiency of the protocol was validated using size exclusion chromatography, MALDI-TOF mass spectrometry, reversed-phase HPLC, and sandwich immunoassay. Furthermore, it was demonstrated that the final eluate of the affinity column is of exceptional purity (98.4%) determined by HPLC and detection at 220 nm. The scale-up of our proposed method seems to be straightforward, which opens the way to the large-scale production of such nanomaterials. This highly improved protocol may facilitate the use and implementation of plant viruses as nanotechnological platforms for in vitro and in vivo applications.</abstract>
    <abstract language="deu">Das Cowpea Chlorotic Mottle Virus (CCMV) ist ein Pflanzenvirus, das als nanotechnologische Plattform erforscht wird. Der robuste Selbstorganisationsmechanismus seines Kapsidproteins ermöglicht die Verkapselung und gezielte Abgabe von Medikamenten. Darüber hinaus kann das Kapsid-Nanopartikel als programmierbare Plattform für die Präsentation verschiedener molekularer Komponenten verwendet werden. Im Hinblick auf künftige Anwendungen ist eine effiziente Produktion und Reinigung von Pflanzenviren von entscheidender Bedeutung. In etablierten Protokollen stellt die notwendige Ultrazentrifugation aufgrund von Kosten, schwieriger Skalierbarkeit und Sicherheitsaspekten eine erhebliche Einschränkung dar. Darüber hinaus bleibt die Reinheit des endgültigen Virusisolats oft unklar. Hier wurde ein fortschrittliches Protokoll für die Reinigung von CCMV aus infiziertem Pflanzengewebe entwickelt, wobei der Schwerpunkt auf Effizienz, Wirtschaftlichkeit und Reinheit lag. Das Protokoll beinhaltet eine Fällung mit Polyethylenglycol (PEG 8000), gefolgt von einer Affinitätsextraktion mit einem neuartigen Peptid-Aptamer. Die Effizienz des Protokolls wurde mithilfe von Größenausschluss-Chromatographie (SEC), MALDI-TOF-Massenspektrometrie, Umkehrphasen-HPLC und Sandwich-Immunoassay validiert. Darüber hinaus wurde nachgewiesen, dass das endgültige Eluat der Affinitätssäule eine außergewöhnliche Reinheit (98,4 %) aufweist, die durch HPLC und Detektion bei 220 nm bestimmt wurde. Die Skalierung der von uns vorgeschlagenen Methode scheint einfach zu sein, was den Weg für eine größer angelegte Produktion solcher Nanomaterialien ebnet. Dieses stark verbesserte Protokoll könnte die Verwendung und Umsetzung von Pflanzenviren als nanotechnologische Plattformen für In-vitro- und In-vivo-Anwendungen erleichtern.</abstract>
    <parentTitle language="eng">Viruses</parentTitle>
    <identifier type="doi">10.3390/v15030697</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-572645</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <enrichment key="date_peer_review">03.04.2023</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Georg Tscheuschner</author>
    <author>Marco Ponader</author>
    <author>Christopher Raab</author>
    <author>Prisca S. Weider</author>
    <author>Reni Hartfiel</author>
    <author>Jan Ole Kaufmann</author>
    <author>Jule L. Völzke</author>
    <author>Gaby Bosc-Bierne</author>
    <author>Carsten Prinz</author>
    <author>T. Schwaar</author>
    <author>Paul Andrle</author>
    <author>Henriette Bäßler</author>
    <author>Khoa Nguyen</author>
    <author>Y. Zhu</author>
    <author>A. S. J. S. Mey</author>
    <author>A. Mostafa</author>
    <author>I. Bald</author>
    <author>Michael G. Weller</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Affinity chromatography</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanoparticles</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanoscience</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Carrier protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Encapsulation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Combinatorial peptide library</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Peptide binder</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Vigna unguiculata</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Augenbohne</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Schlangenbohne</value>
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      <language>deu</language>
      <type>uncontrolled</type>
      <value>Pflanzenvirus</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Plant virus</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Upscaling</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Commercialization</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference material</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanocarrier</value>
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  <doc>
    <id>62158</id>
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    <publishedYear>2024</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>456</pageLast>
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    <edition/>
    <issue/>
    <volume>78</volume>
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    <publisherName>Sage</publisherName>
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    <creatingCorporation>Society for Applied Spectroscopy</creatingCorporation>
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    <title language="eng">Landmark Publications in Analytical Atomic Spectrometry: Fundamentals and&#13;
Instrumentation Development</title>
    <abstract language="eng">The almost-two-centuries history of spectrochemical analysis has generated a body of literature so vast that it has become nearly intractable for experts, much less for those wishing to enter the field. Authoritative, focused reviews help to address this problem but become so granular that the overall directions of the field are lost. This broader perspective can be provided partially by general overviews but then the thinking, experimental details, theoretical underpinnings and instrumental innovations of the original work must be sacrificed. In the present compilation, this dilemma is overcome by assembling the most impactful publications in the area of analytical atomic spectrometry. Each entry was proposed by at least one current expert in the field and supported by a narrative that justifies its inclusion. The entries were then assembled into a coherent sequence and returned to contributors for a round-robin review.</abstract>
    <parentTitle language="eng">Applied spectroscopy</parentTitle>
    <identifier type="issn">1943-3530</identifier>
    <identifier type="doi">10.1177/00037028241263567</identifier>
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    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>George C.-Y. Chan</author>
    <author>Gary M. Hieftje</author>
    <author>Nicoló Omenetto</author>
    <author>Ove Axner</author>
    <author>Arne Bengtson</author>
    <author>Nicolas H. Bings</author>
    <author>Michael W. Blades</author>
    <author>Annemie Bogaerts</author>
    <author>Mikhail A. Bolshov</author>
    <author>José A.C. Broekaert</author>
    <author>WingTat Chan</author>
    <author>José M. Costa-Fernández</author>
    <author>Stanley R. Crouch</author>
    <author>Alessandro De Giacomo</author>
    <author>Alessandro D’Ulivo</author>
    <author>Carsten Engelhard</author>
    <author>Heinz Falk</author>
    <author>Paul B. Farnsworth</author>
    <author>Stefan Florek</author>
    <author>Gerardo Gamez</author>
    <author>Igor B. Gornushkin</author>
    <author>Detlef Günther</author>
    <author>David W. Hahn</author>
    <author>Wei Hang</author>
    <author>Volker Hoffmann</author>
    <author>Norbert Jakubowski</author>
    <author>Vassili Karanassios</author>
    <author>David W. Koppenaal</author>
    <author>R. Kenneth Marcus</author>
    <author>Reinhard Noll</author>
    <author>John W. Olesik</author>
    <author>Vincenzo Palleschi</author>
    <author>Ulrich Panne</author>
    <author>Jorge Pisonero</author>
    <author>Steven J. Ray</author>
    <author>Martín Resano</author>
    <author>Richard E. Russo</author>
    <author>Alexander Scheeline</author>
    <author>Benjamin W. Smith</author>
    <author>Ralph E. Sturgeon</author>
    <author>José-Luis Todolí</author>
    <author>Elisabetta Tognoni</author>
    <author>Frank Vanhaecke</author>
    <author>Michael R. Webb</author>
    <author>James D. Winefordner</author>
    <author>Lu Yang</author>
    <author>Jin Yu</author>
    <author>Zhanxia Zhang</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Analytical atomic spectrometry</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Spectroscopy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Instrumental analysis</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">P Präsident</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.3 Instrumentelle Analytik</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <collection role="institutes" number="">P.0 Präsident und andere</collection>
    <collection role="institutes" number="">1.0 Abteilungsleitung und andere</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/62158/2025_as-79-4-481_final_version.pdf</file>
  </doc>
  <doc>
    <id>57293</id>
    <completedYear/>
    <publishedYear>2023</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>5185</pageFirst>
    <pageLast>5195</pageLast>
    <pageNumber/>
    <edition/>
    <issue>7</issue>
    <volume>66</volume>
    <type>article</type>
    <publisherName>ACS Publications</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">NIR-II Aza-BODIPY Dyes Bioconjugated to Monoclonal Antibody Trastuzumab for Selective Imaging of HER2-Positive Ovarian Cancer</title>
    <abstract language="eng">Using fluorescence-guided surgery (FGS) to cytoreductive surgery helps achieving complete resection of microscopic ovarian tumors. The use of visible and NIR-I fluorophores has led to beneficial results in clinical trials; however, involving NIR-II dyes seems to outperform those benefits due to the deeper tissue imaging and higher signal/noise ratio attained within the NIR-II optical window. In this context, we developed NIR-II emitting dyes targeting human epidermal growth factor receptor 2 (HER2)-positive ovarian tumors by coupling water-soluble NIR-II aza-BODIPY dyes to the FDA-approved anti-HER2 antibody, namely, trastuzumab. These bioconjugated NIR-II-emitting dyes displayed a prolonged stability in serum and a maintained affinity toward HER2 in vitro. We obtained selective targeting of HER2 positive tumors (SKOV-3) in vivo, with a favorable tumor accumulation. We demonstrated the fluorescence properties and the specific HER2 binding of the bioconjugated dyes in vivo and thus their potential for NIR-II FGS in the cancer setting.</abstract>
    <parentTitle language="eng">Journal of Medicinal Chemistry</parentTitle>
    <identifier type="doi">10.1021/acs.jmedchem.3c00100</identifier>
    <identifier type="issn">0022-2623</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">13.04.2023</enrichment>
    <author>A. Godard</author>
    <author>G. Kalot</author>
    <author>M. Privat</author>
    <author>M. Bendellaa</author>
    <author>B. Busser</author>
    <author>Karl David Wegner</author>
    <author>F. Denat</author>
    <author>X. Le Guevel</author>
    <author>J.-L. Coll</author>
    <author>C. Paul</author>
    <author>E. Bodio</author>
    <author>C. Goze</author>
    <author>L. Sancey</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>NIR-II</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fluorescent dye</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>In vivo imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ovarian cancer</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Antibody conjuagtes</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Bioimaging</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.2 Biophotonik</collection>
    <collection role="themenfelder" number="">Material</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="themenfelder" number="">Materialdesign</collection>
  </doc>
  <doc>
    <id>59607</id>
    <completedYear/>
    <publishedYear>2024</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>3679</pageFirst>
    <pageLast>3691</pageLast>
    <pageNumber/>
    <edition/>
    <issue>5</issue>
    <volume>67</volume>
    <type>article</type>
    <publisherName>ACS Publications</publisherName>
    <publisherPlace>Washington, DC</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Comparison of the In Vitro and In Vivo Behavior of a Series of NIR-II-Emitting Aza-BODIPYs Containing Different Water-Solubilizing Groups and Their Trastuzumab Antibody Conjugates</title>
    <abstract language="eng">The development of new fluorescent organic probes effective in the NIR-II region is currently a fast-growing field and represents a challenge in the domain of medical imaging. In this study, we have designed and synthesized an innovative series of aza-boron dipyrromethenes emitting in the NIR-II region. We have investigated the effect of different water-solubilizing groups not only on the photophysical properties of the compounds but also on their in vitro and in vivo performance after bioconjugation to the antibody trastuzumab. Remarkably, we discovered that the most lipophilic compound unexpectedly displayed the most favorable in vivo properties after bioconjugation. This underlines the profound influence that the fluorophore functionalization approach can have on the efficiency of the resulting imaging agent.</abstract>
    <parentTitle language="eng">Journal of Medicinal Chemistry</parentTitle>
    <identifier type="doi">10.1021/acs.jmedchem.3c02139</identifier>
    <identifier type="issn">1520-4804</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">06.03.2024</enrichment>
    <author>E. Chazeau</author>
    <author>C. Fabre</author>
    <author>M. Privat</author>
    <author>A. Godard</author>
    <author>C. Racoeur</author>
    <author>E. Bodio</author>
    <author>B. Busser</author>
    <author>Karl David Wegner</author>
    <author>L. Sancey</author>
    <author>C. Paul</author>
    <author>C. Goze</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>NIR-II</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>In vivo imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fluorescence</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Spectroscopy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Antibody conjugates</value>
    </subject>
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    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.2 Biophotonik</collection>
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  </doc>
  <doc>
    <id>63025</id>
    <completedYear/>
    <publishedYear>2025</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>7232</pageFirst>
    <pageLast>7242</pageLast>
    <pageNumber/>
    <edition/>
    <issue>7</issue>
    <volume>68</volume>
    <type>article</type>
    <publisherName>ACS Publications</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">NIR-II aza-BODIPY Platform for the Development of a Fluorescent Antibody Drug Conjugate</title>
    <abstract language="eng">Real-time imaging of antibody-drug conjugates (ADCs) offers valuable insights for assessing tumor targeting specificity, monitoring therapeutic efficacy, and detecting off-target accumulation that may cause adverse effects. To enable precise tracking, we developed a versatile fluorescent platform based on an NIR-II emitting aza-BODIPY dye, which can be site-specifically grafted onto an IgG1 antibody to generate well-defined fluorescent ADCs. As a proof of concept, we synthesized an HER2-targeting trastuzumab immunoconjugate bearing a NIR-II aza-BODIPY fluorophore. The cytotoxic monomethyl auristatin E (MMAE) payload was introduced in the final step, resulting in a trackable and homogeneous ADC suitable for both in vitro and in vivo investigations. The resulting Trastu-azaNIRII-MMAE selectively accumulated in HER2-positive subcutaneous tumors, significantly reducing the tumor growth. Using NIR-II optical imaging, a single injection of the NIR-II-ADC allowed for the detection of the conjugate over a period of more than one month, highlighting its potential for long-term tracking and therapeutic applications.</abstract>
    <parentTitle language="eng">Journal of Medicinal Chemistry</parentTitle>
    <identifier type="doi">10.1021/acs.jmedchem.4c02777</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">05.05.2025</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <author>E. Chazeau</author>
    <author>A. Pipier</author>
    <author>Karl David Wegner</author>
    <author>F. Ghiringhelli</author>
    <author>L. Sancey</author>
    <author>C. Paul</author>
    <author>C. Goze</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>NIR-II</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fluorescence</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Quality assurance</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Antibody drug conjugate</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>In vivo imaging</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.2 Biophotonik</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
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    <file>https://opus4.kobv.de/opus4-bam/files/63025/Chazeau_JMedChem_2025.pdf</file>
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    <publishedYear>2020</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>781</pageFirst>
    <pageLast>791</pageLast>
    <pageNumber/>
    <edition/>
    <issue>6</issue>
    <volume>12</volume>
    <type>article</type>
    <publisherName>Royal Society of Chemistry</publisherName>
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    <belongsToBibliography>1</belongsToBibliography>
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    <title language="eng">Exploratory analysis of hyperspectral FTIR data obtained from environmental microplastics samples</title>
    <abstract language="eng">Hyperspectral imaging of environmental samples with infrared microscopes is one of the preferred methods to find and characterize microplastics. Particles can be quantified in terms of number, size and size distribution. Their shape can be studied and the substances can be identified. Interpretation of the collected spectra is a typical problem encountered during the analysis. The image datasets are large and contain spectra of countless particles of natural and synthetic origin. To supplement existing Analysis pipelines, exploratory multivariate data analysis was tested on two independent datasets. Dimensionality reduction with principal component analysis (PCA) and uniform manifold approximation and projection (UMAP) was used as a core concept. It allowed for improved visual accessibility of the data and created a chemical two-dimensional image of the sample. Spectra belonging to particles could be separated from blank spectra, reducing the amount of data significantly. Selected spectra were further studied, also applying PCA and UMAP. Groups of similar spectra were identified by cluster analysis using k-means, density based, and interactive manual clustering. Most clusters could be assigned to chemical species based on reference spectra. While the results support findings obtained with a ‘targeted analysis’ based on automated library search, exploratory analysis points the attention towards the group of unidientified spectra that remained and are otherwise easily overlooked.</abstract>
    <parentTitle language="eng">Analytical Methods</parentTitle>
    <identifier type="doi">10.1039/c9ay02483b</identifier>
    <enrichment key="date_peer_review">05.03.2020</enrichment>
    <author>Lukas Wander</author>
    <author>A. Vianello</author>
    <author>J. Vollertsen</author>
    <author>F. Westad</author>
    <author>Ulrike Braun</author>
    <author>Andrea Paul</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Microplastics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>FTIR</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Exploratory analysis</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.4 Non-Target-Analytik</collection>
    <collection role="institutes" number="">6 Materialchemie</collection>
    <collection role="institutes" number="">6.6 Digitale Materialchemie</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
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    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>55147</id>
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    <publishedYear>2022</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>10089</pageFirst>
    <pageLast>10105</pageLast>
    <pageNumber/>
    <edition/>
    <issue>11</issue>
    <volume>22</volume>
    <type>article</type>
    <publisherName>IEEE</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
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    <title language="eng">Silicon Photonic Micro-Ring Resonators for Chemical and Biological Sensing: A Tutorial</title>
    <abstract language="eng">Silicon photonic micro-ring resonators (MRR) developed on the silicon-on-insulator (SOI) platform, owing to their high sensitivity and small footprint, show great potential for many chemical and biological sensing applications such as label-free detection in environmental monitoring, biomedical engineering, and food analysis. In this tutorial,we provide the theoretical background and give design guidelines for SOI-based MRR as well as examples of surface functionalization procedures for label-free detection of molecules. After introducing the advantages and perspectives of MRR, fundamentals of MRR are described in detail, followed by an introduction to the fabrication methods, which are based on a complementary metal-oxide semiconductor (CMOS) technology. Optimization of MRR for chemical and biological sensing is provided, with special emphasis on the optimization of waveguide geometry. At this point, the difference between chemical bulk sensing and label-free surface sensing is explained, and definitions like waveguide sensitivity, ring sensitivity, overall sensitivity as well as the limit of detection (LoD) of MRR are introduced. Further, we show and explain chemical bulk sensing of sodium chloride (NaCl) in water and provide a recipe for label-free surface sensing.</abstract>
    <parentTitle language="eng">IEEE Sensors Journal</parentTitle>
    <identifier type="doi">10.1109/JSEN.2021.3119547</identifier>
    <identifier type="issn">1530-437X</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">30.06.2022</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <author>P. Steglich</author>
    <author>D. G. Rabus</author>
    <author>C. Sada</author>
    <author>Martin Paul</author>
    <author>Michael G. Weller</author>
    <author>C. Mai</author>
    <author>A. Mai</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biosensors</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biophotonics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Chemosensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biosensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Microresonator</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanophotonics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Optical sensors</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Photonic sensors</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Optoelectronic</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ring resonator</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Silicon photonics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Miniaturization</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lab-on-a-chip</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lab-on-chip</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Waveguide</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Surface chemistry</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Silanization</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Glutaraldehyde</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Affinity immobilization</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Antibody</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Oriented immobilization</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Real-time measurement</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.5 Proteinanalytik</collection>
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    <id>51713</id>
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    <publishedYear>2020</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1241</pageFirst>
    <pageLast>1244</pageLast>
    <pageNumber/>
    <edition/>
    <issue>19</issue>
    <volume>32</volume>
    <type>article</type>
    <publisherName>IEEE</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">CMOS-Compatible Silicon Photonic Sensor for Refractive Index Sensing Using Local Back-Side Release</title>
    <abstract language="eng">Silicon photonic sensors are promising candidates for lab-on-a-chip solutions with versatile applications and scalable production prospects using complementary metal-oxide semiconductor (CMOS) fabrication methods. However, the widespread use has been hindered because the sensing area adjoins optical and electrical components making packaging and sensor handling challenging. In this work, a local back-side release of the photonic sensor is employed, enabling a separation of the sensing area from the rest of the chip. This approach allows preserving the compatibility of photonic integrated circuits in the front-end of line and metal interconnects in the back-end of line. The sensor is based on a micro-ring resonator and is fabricated on wafer-level using a CMOS technology. We revealed a ring resonator sensitivity for homogeneous sensing of 106 nm/RIU.</abstract>
    <parentTitle language="eng">IEEE Photonics Technology Letters</parentTitle>
    <identifier type="doi">10.1109/LPT.2020.3019114</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-517139</identifier>
    <enrichment key="date_peer_review">17.12.2020</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>P. Steglich</author>
    <author>S. Bondarenko</author>
    <author>C. Mai</author>
    <author>Martin Paul</author>
    <author>Michael G. Weller</author>
    <author>A. Mai</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Photonic biosensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lab-on-a-chip</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ring resonator</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Resonance wavelength shift</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>PIC technology</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Back-side integration</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.5 Proteinanalytik</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
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    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/51713/Steglich-2020-IEEEPhotonicsTechnolLetters.pdf</file>
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    <publishedYear>2020</publishedYear>
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    <completedDate>--</completedDate>
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    <title language="eng">BioPIC - Integration of Biosensors based on Photonic Integrated Circuits by Local-Backside Etching</title>
    <abstract language="eng">Silicon photonic sensors are promising candidates for lab-on-a-chip solutions with versatile applications and scalable production prospects using complementary metal-oxide semiconductor (CMOS) fabrication methods. However, the widespread use has been hindered because the sensing area adjoins optical and electrical components making packaging and sensor handling challenging. In this work, a local back-side release of the photonic sensor is employed, enabling a separation of the sensing area from the rest of the chip. This approach allows preserving the compatibility of photonic integrated circuits in the front-end of line and metal interconnects in the back-end of line.</abstract>
    <parentTitle language="eng">ATTRACT Showroom</parentTitle>
    <identifier type="url">https://attract-eu.com/showroom/project/integration-of-biosensors-based-on-photonic-integrated-circuits-by-local-backside-etching-biopic/</identifier>
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    <enrichment key="eventPlace">Online meeting</enrichment>
    <enrichment key="eventStart">22.09.2020</enrichment>
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    <author>P. Steglich</author>
    <author>C. Mai</author>
    <author>S. Bondarenko</author>
    <author>Martin Paul</author>
    <author>Michael G. Weller</author>
    <author>S. Schrader</author>
    <author>A. Mai</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Silicon Photonics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Photonic Sensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Photonic Integrated Circuits</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Point-Of-Care-Diagnostics</value>
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    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>CMOS</value>
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    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Microfluidics</value>
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    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lab-on-a-chip</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ring resonator</value>
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    <collection role="ddc" number="543">Analytische Chemie</collection>
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    <publisherName>IEEE</publisherName>
    <publisherPlace>Piscataway Township</publisherPlace>
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    <title language="eng">Silicon Photonic Micro-Ring Resonators for Chemical and Biological Sensing: A Tutorial</title>
    <abstract language="eng">Silicon photonic micro-ring resonators (MRR) developed on the silicon-on-insulator (SOI) platform, owing to their high sensitivity and small footprint, show great potential for many chemical and biological sensing applications such as label-free detection in environmental monitoring, biomedical engineering, and food analysis. In this tutorial, we provide the theoretical background and give design guidelines for SOI-based MRR as well as examples of surface functionalization procedures for label-free detection of molecules.&#13;
After introducing the advantages and perspectives of MRR, fundamentals of MRR are described in detail, followed by an introduction to the fabrication methods, which are based on a complementary metal-oxide semiconductor (CMOS) technology. Optimization of MRR for chemical and biological sensing is provided, with special emphasis on the optimization of waveguide geometry. At this point, the difference between chemical bulk sensing and label-free surface sensing is explained, and definitions like waveguide sensitivity, ring sensitivity, overall sensitivity as well as the limit of detection (LoD) of MRR are introduced. Further, we show and explain chemical bulk sensing of sodium chloride (NaCl) in water and provide a recipe for label-free surface sensing.</abstract>
    <parentTitle language="eng">TechRixv</parentTitle>
    <identifier type="doi">10.36227/techrxiv.14909901.v1</identifier>
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    <author>D. G. Rabus</author>
    <author>C. Sada</author>
    <author>Martin Paul</author>
    <author>Michael G. Weller</author>
    <author>C. Mai</author>
    <author>A. Mai</author>
    <subject>
      <language>eng</language>
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      <value>Lab on a chip</value>
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      <language>eng</language>
      <type>uncontrolled</type>
      <value>Silanization</value>
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      <language>eng</language>
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      <value>Surface derivatization</value>
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    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Evanescent wave</value>
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    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.5 Proteinanalytik</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
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    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <collection role="themenfelder" number="">Sensorik</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/52936/Steglich-2021-TechRxiv-Tutorial.pdf</file>
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