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<export-example>
  <doc>
    <id>32981</id>
    <completedYear/>
    <publishedYear>2015</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>254</pageFirst>
    <pageLast>261</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>87</volume>
    <type>article</type>
    <publisherName>Wiley-Liss</publisherName>
    <publisherPlace>Hoboken, NJ</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Quantification of cells with specific phenotypes II: Determination of CD4 expression level on reconstituted lyophilized human PBMC labelled with anti-CD4 FITC antibody</title>
    <abstract language="eng">This report focuses on the characterization of CD4 expression level in terms of equivalent number of reference fluorophores (ERF). Twelve different flow cytometer platforms across sixteen laboratories were utilized in this study. As a first step the participants were asked to calibrate the fluorescein isothiocyanate (FITC) channel of each flow cytometer using commercially available calibration standard consisting of five populations of microspheres. Each population had an assigned value of equivalent fluorescein fluorophores (EFF denotes a special case of the generic term ERF with FITC as the reference fluorophore). The EFF values were assigned at the National Institute of Standards and Technology (NIST). A surface-labelled lyophilized cell preparation was provided by the National Institute of Biological Standards and Control (NIBSC), using human peripheral blood mononuclear cells (PBMC) pre-labeled with a FITC conjugated anti-CD4 monoclonal antibody. Three PBMC sample vials, provided to each participant, were used for the CD4 expression analysis. The PBMC are purported to have a fixed number of surface CD4 receptors. On the basis of the microsphere calibration, the EFF value of the PBMC samples was measured to characterize the population average CD4 expression level of the PBMC preparations. Both the results of data analysis performed by each participant and the results of centralized analysis of all participants' raw data are reported. Centralized analysis gave a mean EFF value of 22,300 and an uncertainty of 750, corresponding to 3.3% (level of confidence 68%) of the mean EFF value. The next step will entail the measurement of the ERF values of the lyophilized PBMC stained with labels for other fluorescence channels. The ultimate goal is to show that lyophilized PBMC is a suitable biological reference cell material for multicolor flow cytometry and that it can be used to present multicolor flow cytometry measurements in terms of ABC (antibodies bound per cell) units.</abstract>
    <parentTitle language="eng">Cytometry / A</parentTitle>
    <identifier type="old">36060</identifier>
    <identifier type="doi">10.1002/cyto.a.22634</identifier>
    <identifier type="issn">0196-4763</identifier>
    <identifier type="issn">1552-4922</identifier>
    <identifier type="issn">1552-4930</identifier>
    <author>L. Wang</author>
    <author>R. Stebbings</author>
    <author>A.K. Gaigalas</author>
    <author>J. Sutherland</author>
    <author>M. Kammel</author>
    <author>M. John</author>
    <author>B. Roemer</author>
    <author>Maren Kuhne</author>
    <author>Rudolf Schneider</author>
    <author>M. Braun</author>
    <author>A. Engel</author>
    <author>D. Dikshit</author>
    <author>F. Abbasi</author>
    <author>G.E. Marti</author>
    <author>M. Sassi</author>
    <author>L. Revel</author>
    <author>S.K. Kim</author>
    <author>M.-O. Baradez</author>
    <author>T. Lekishvili</author>
    <author>D. Marshall</author>
    <author>L. Whitby</author>
    <author>W. Jing</author>
    <author>V. Ost</author>
    <author>M. Vonsky</author>
    <author>J. Neukammer</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Surface labelled lyophilized PBMC</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>CD4 expression level</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>FITC</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Equivalent fluorescein fluorophore (EFF)</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Quantitative flow cytometry</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Calibration</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Standard measurement procedure</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Measurement uncertainty</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference cell material</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>32847</id>
    <completedYear/>
    <publishedYear>2015</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>244</pageFirst>
    <pageLast>253</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>87</volume>
    <type>article</type>
    <publisherName>Wiley-Liss</publisherName>
    <publisherPlace>Hoboken, NJ</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Quantification of cells with specific phenotypes I: Determination of CD4+ cell count per microliter in reconstituted lyophilized human PBMC prelabeled with anti-CD4 FICT antibody</title>
    <abstract language="eng">A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells prelabeled with a fluorescein isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL preparation is intended to be used as a reference material for CD4+ cell counting including the development of higher order reference measurement procedures and has been evaluated in the pilot study CCQM-P102. This study was conducted across 16 laboratories from eight countries to assess the ability of participants to quantify the CD4+ cell count of this reference material and to document cross-laboratory variability plus associated measurement uncertainties. Twelve different flow cytometer platforms were evaluated using a standard protocol that included calibration beads used to obtain quantitative measurements of CD4+ T cell counts. There was good overall cross-platform and counting method agreement with a grand mean of the laboratory calculated means of (301.7 ± 4.9) µL-1 CD4+ cells. Excluding outliers, greater than 90% of participant data agreed within ±15%. A major contribution to variation of sLL CD4+ cell counts was tube to tube variation of the calibration beads, amounting to an uncertainty of 3.6%. Variation due to preparative steps equated to an uncertainty of 2.6%. There was no reduction in variability when data files were centrally reanalyzed. Remaining variation was attributed to instrument specific differences. CD4+ cell counts obtained in CCQM-P102 are in excellent agreement and show the robustness of both the measurements and the data analysis and hence the suitability of sLL as a reference material for interlaboratory comparisons and external quality assessment.</abstract>
    <parentTitle language="eng">Cytometry / A</parentTitle>
    <identifier type="old">35923</identifier>
    <identifier type="doi">10.1002/cyto.a.22614</identifier>
    <identifier type="issn">0196-4763</identifier>
    <identifier type="issn">1552-4922</identifier>
    <identifier type="issn">1552-4930</identifier>
    <author>R. Stebbings</author>
    <author>L. Wang</author>
    <author>J. Sutherland</author>
    <author>M. Kammel</author>
    <author>A.K. Gaigalas</author>
    <author>M. John</author>
    <author>B. Roemer</author>
    <author>Maren Kuhne</author>
    <author>Rudolf Schneider</author>
    <author>M. Braun</author>
    <author>A. Engel</author>
    <author>D.K. Dikshit</author>
    <author>F. Abbasi</author>
    <author>G.E. Marti</author>
    <author>M.P. Sassi</author>
    <author>L. Revel</author>
    <author>S.-K. Kim</author>
    <author>M.-O. Baradez</author>
    <author>T. Lekishvili</author>
    <author>D. Marshall</author>
    <author>L. Whitby</author>
    <author>W. Jing</author>
    <author>V. Ost</author>
    <author>M. Vonsky</author>
    <author>J. Neukammer</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>CD4+ cell counting</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Relative concentration measurement</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lyophilized cells</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Flow cytometry</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Standard measurement procedure</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Measurement of uncertainty</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Human immunodeficiency virus-1</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Acquired immunodeficiency syndrome</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference material</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>23196</id>
    <completedYear/>
    <publishedYear>2010</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>293</pageFirst>
    <pageLast>300</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>80</volume>
    <type>article</type>
    <publisherName>Elsevier Science</publisherName>
    <publisherPlace>Kidlington, Oxford</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Effect of long term organic amendments on adsorption-desorption of thiram onto a luvisol soil derived from loess</title>
    <parentTitle language="eng">Chemosphere</parentTitle>
    <identifier type="old">25767</identifier>
    <identifier type="doi">10.1016/j.chemosphere.2010.04.003</identifier>
    <identifier type="issn">0045-6535</identifier>
    <identifier type="issn">0366-7111</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">08.02.2011</enrichment>
    <author>O.M.S. Filipe</author>
    <author>M.M. Vidal</author>
    <author>H.W. Scherer</author>
    <author>Rudolf Schneider</author>
    <author>A. Duarte</author>
    <author>V.I. Esteves</author>
    <author>E.B.H. Santos</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Thiram</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Adsorption-desorption</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Soil</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Organic amendments</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>54972</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>08001</pageFirst>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>1A</issue>
    <volume>59</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">PAWG Pilot Study on Quantification of SARS-CoV-2 Monoclonal Antibody - Part 1</title>
    <abstract language="deu">Under the auspices of the Protein Analysis Working Group (PAWG) of the Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P216, was coordinated by the Chinese National Institute of Metrology (NIM), National Research Council of Canada (NRC) and the Bureau International des Poids et Mesures (BIPM). Eleven Metrology Institutes or Designated Institutes and the BIPM participated in the first phase of the pilot study (Part 1). The purpose of this pilot study was to develop measurement capabilities for larger proteins using a recombinant humanized IgG monoclonal antibody against Spike glycoprotein of SARS-CoV-2 (Anti-S IgG mAb) in solution. The first phase of the study was designed to employ established methods that had been previously studies by the CCQM Protein Analysis Working Group, involving the digestion of protein down to the peptide or amino acid level. The global coronavirus pandemic has also led to increased focus on antibody quantitation methods. IgG are among the immunoglobulins produced by the immune system to provide protection against SARS-CoV-2. Anti-SARS-CoV-2 IgG can therefore be detected in samples from affected patients. Antibody tests can show whether a person has been exposed to the SARS-CoV-2, and whether or not they potentially show lasting immunity to the disease. With the constant spread of the virus and the high pressure of re-opening economies, antibody testing plays a critical role in the fight against COVID-19 by helping healthcare professionals to identify individuals who have developed an immune response, either via vaccination or exposure to the virus. Many countries have launched large-scale antibody testing for COVID-19. The development of measurement standards for the antibody detection of SARS-CoV-2 is critically important to deal with the challenges of the COVID-19 pandemic. In this study, the SARS-CoV-2 monoclonal antibody is being used as a model system to build capacity in methods that can be used in antibody quantification. Amino acid reference values with corresponding expanded uncertainty of 36.10 ± 1.55 mg/kg, 38.75 ± 1.45 mg/kg, 18.46 ± 0.78 mg/kg, 16.20 ± 0.67 mg/kg and 30.61 ± 1.30 mg/kg have been established for leucine, valine, phenylalanine, isoleucine and proline, respectively. Agreement between nearly all laboratories was achieved for the amino acid analysis within 2 to 2.5 %, with one participant achieving markedly higher results due to a technical issue found in their procedure; this result was thus excluded from the reference value calculations. The relatively good agreement within a laboratory between different amino acids was not dissimilar to previous results for peptides or small proteins, indicating that factors such as hydrolysis conditions and calibration procedures could be the largest sources of variability. Peptide reference values with corresponding expanded uncertainty of 4.99 ± 0.28 mg/kg and 6.83 ± 0.65 mg/kg have been established for ALPAPIEK and GPSVFPLAPSSK, respectively. Not surprisingly due to prior knowledge from previous studies on peptide quantitation, agreement between laboratories for the peptide-based analysis was slightly poorer at 3 to 5 %, with one laboratory's result excluded for the peptide GPSVFPLAPSSK. Again, this level of agreement was not significantly poorer than that achieved in previous studies with smaller or less complex proteins. To reach the main text of this paper, click on Final Report.</abstract>
    <parentTitle language="deu">Metrologia</parentTitle>
    <identifier type="doi">10.1088/0026-1394/59/1a/08001</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <author>W. Mi</author>
    <author>R. D. Josephs</author>
    <author>J. E. Melanson</author>
    <author>X. Dai</author>
    <author>Y. Wang</author>
    <author>R. Zhai</author>
    <author>Z. Chu</author>
    <author>X. Fang</author>
    <author>M.-P. Thibeault</author>
    <author>B. B. Stocks</author>
    <author>J. Meija</author>
    <author>M. Bedu</author>
    <author>G. Martos</author>
    <author>S. Westwood</author>
    <author>R. I. Wielgosz</author>
    <author>Q. Liu</author>
    <author>T. L. Teo</author>
    <author>H. Liu</author>
    <author>Y. J. Tan</author>
    <author>M. Öztuğ</author>
    <author>E. Saban</author>
    <author>T. Kinumi</author>
    <author>K. Saikusa</author>
    <author>Rudolf Schneider</author>
    <author>Michael G. Weller</author>
    <author>Zoltán Konthur</author>
    <author>Carsten Jaeger</author>
    <author>M. Quaglia</author>
    <author>C. Mussell</author>
    <author>G. Drinkwater</author>
    <author>C. Giangrande</author>
    <author>H. Vaneeckhoutte</author>
    <author>A. Boeuf</author>
    <author>V. Delatour</author>
    <author>J. E. Lee</author>
    <author>G. O'Connor</author>
    <author>R. Ohlendorf</author>
    <author>A. Henrion</author>
    <author>P. J. Beltrão</author>
    <author>S. M. Naressi Scapin</author>
    <author>Y. B. Sade</author>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Antibody quantification</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Amino acid analysis</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Peptide analysis</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Round robin test</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.5 Proteinanalytik</collection>
    <collection role="institutes" number="">1.8 Umweltanalytik</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
</export-example>
