<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>44588</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>5</pageLast>
    <pageNumber/>
    <edition/>
    <issue>Application Note #000399</issue>
    <volume/>
    <type>other</type>
    <publisherName/>
    <publisherPlace>Berlin</publisherPlace>
    <creatingCorporation>SPECS Surface Nano Analysis GmbH</creatingCorporation>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">XPS surface analysis of bacterial samples</title>
    <abstract language="eng">This application note presents how EnviroESCA can be used to analyze bacterial samples under near ambient pressure conditions in various states of hydration using different levels of humidity. Such investigations of bacterial cell wall surfaces in their hydrated state are essential for studying biological interfaces at work.&#13;
The use of innovative near-ambient pressure (NAP-)XPS instrumentation allows the detailed analysis of irregularly-surfaced biofilms. NAP-XPS enables the surface analysis of bacterial samples in their natural hydrated state without complex sample preparation techniques such as freeze-drying or fast-freezing, which are needed for XPS analysis in ultrahigh vacuum.</abstract>
    <identifier type="url">http://www.enviroai.com/uploads/1/0/2/8/102861712/xps_surface_analysis_of_bacillus_subtilis_biofilms_final.pdf</identifier>
    <author>P. Dietrich</author>
    <author>K. Beblo-Vranesevic</author>
    <author>Marit Kjærvik</author>
    <author>Wolfgang Unger</author>
    <author>Karin Schwibbert</author>
    <author>K. Hardie</author>
    <author>J. Brown</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Near ambient pressure XPS</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biofilms</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="literaturgattung" number="">Graue Literatur</collection>
  </doc>
  <doc>
    <id>45720</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>4</pageLast>
    <pageNumber/>
    <edition/>
    <issue>Application Note #000400</issue>
    <volume/>
    <type>other</type>
    <publisherName/>
    <publisherPlace>Berlin, Germany</publisherPlace>
    <creatingCorporation>SPECS Surface Nano Analysis GmbH</creatingCorporation>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Near-ambient pressure XPS of  hydrated  Escherichia coli samples with EnviroESCA</title>
    <abstract language="eng">This application note presents how EnviroESCA can be used to analyze E. coli biofilms on silicon under near ambient pressure conditions in various states of hydration. Such investigations of the outer bacterial cell surface in their hydrated state are essential for studying biological interfaces at work.</abstract>
    <identifier type="url">http://www.enviroai.com/uploads/1/0/2/8/102861712/nap_xps_of_escherichia_coli_samples.pdf</identifier>
    <author>Marit Kjaervik</author>
    <author>Karin Schwibbert</author>
    <author>P. Dietrich</author>
    <author>A. Thissen</author>
    <author>Wolfgang Unger</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biofilms</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>E. coli</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>NAP-XPS</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="literaturgattung" number="">Graue Literatur</collection>
  </doc>
  <doc>
    <id>45404</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>996</pageFirst>
    <pageLast>1000</pageLast>
    <pageNumber/>
    <edition/>
    <issue>11</issue>
    <volume>50</volume>
    <type>article</type>
    <publisherName>John Wiley &amp; Sons</publisherName>
    <publisherPlace>Hoboken, New Jersey, USA</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Surface characterisation of Escherichia coli under various conditions by near-ambient pressure XPS</title>
    <abstract language="eng">Bacteria are inherently in a hydrated state and therefore not compatible to ultra-high vacuum techniques such as XPS without prior sample preparation involving freeze drying or fast freezing.&#13;
This has changed with the development of near-ambient pressure (NAP)-XPS, which makes it possible to characterise the bacterial surface with minimal sample preparation. This paper presents NAP-XPS measurements of Escherichia coli under various NAP conditions: at 11 mbar in a humid environment, at 2 mbar after drying in the chamber, pre-dried at 4 mbar, and at 1 mbar after overnight pumping at 10^−4 mbar. The high-resolution spectra of carbon, nitrogen, and oxygen are presented and found to be in general agreement with XPS measurements from freeze-dried and fast-frozen bacteria. However, it was found that the amount of carbon components associated with polysaccharides increases relative to aliphatic carbon during drying and increases further after overnight pumping. This implies that drying has an impact on the bacterial surface.</abstract>
    <parentTitle language="eng">Surface and Interface Analysis</parentTitle>
    <identifier type="doi">10.1002/sia.6480</identifier>
    <identifier type="issn">0142-2421</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-454047</identifier>
    <identifier type="issn">1096-9918</identifier>
    <enrichment key="eventName">European conference on applications of surface and interface analysis (ECASIA'17)</enrichment>
    <enrichment key="eventPlace">Montpellier, France</enrichment>
    <enrichment key="eventStart">24.09.2018</enrichment>
    <enrichment key="eventEnd">29.09.2018</enrichment>
    <enrichment key="date_peer_review">15.11.2018</enrichment>
    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>Marit Kjaervik</author>
    <author>Karin Schwibbert</author>
    <author>P. Dietrich</author>
    <author>A. Thissen</author>
    <author>Wolfgang Unger</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Bacteria</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>E. coli</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>NAP-XPS</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/45404/Kj-rvik_et_al-2018-Surface_and_Interface_Analysis.pdf</file>
  </doc>
</export-example>
