<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>47432</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>992</pageFirst>
    <pageLast>1013</pageLast>
    <pageNumber/>
    <edition/>
    <issue>9</issue>
    <volume>12</volume>
    <type>article</type>
    <publisherName>Taylor &amp; Francis</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Uptake and molecular impact of aluminum-containing nanomaterials on human intestinal caco-2 cells</title>
    <abstract language="eng">Aluminum (Al) is one of the most common elements in the earth crust and increasingly used in food, consumer products and packaging. Its hazard potential for humans is still not completely understood. Besides the metallic form, Al also exists as mineral, including the insoluble oxide, and in soluble ionic forms. Representatives of these three species, namely a metallic and an oxidic species of Al-containing nanoparticles and soluble aluminum chloride, were applied to human intestinal cell lines as models for the intestinal barrier. We characterized physicochemical particle parameters, protein corona composition, ion release and cellular uptake. Different in vitro assays were performed to determine potential effects and molecular modes of Action related to the individual chemical species. For a deeper insight into signaling processes, microarray transcriptome analyses followed by bioinformatic data analysis were employed. The particulate Al species showed different solubility in biological media. Metallic Al nanoparticles released more ions than Al2O3 nanoparticles, while AlCl3 showed a mixture of dissolved and agglomerated particulate entities in biological media. The protein corona composition differed between both nanoparticle species. Cellular uptake, investigated in transwell experiments, occurred predominantly in particulate form, whereas ionic Al was not taken up by intestinal cell lines. Transcellular transport was not observed. None of the Al species showed cytotoxic effects up to 200 mg Al/mL. The transcriptome analysis indicated mainly effects on oxidative stress pathways, xenobiotic metabolism and metal homeostasis. We have shown for the first time that intestinal cellular uptake of Al occurs preferably in the particle form, while toxicological effects appear to be ion-related.</abstract>
    <parentTitle language="eng">Nanotoxicology</parentTitle>
    <identifier type="doi">10.1080/17435390.2018.1504999</identifier>
    <identifier type="issn">1743-5390</identifier>
    <enrichment key="date_peer_review">25.02.2019</enrichment>
    <author>H. Sieg</author>
    <author>C. Braeuning</author>
    <author>B. M. Kunz</author>
    <author>H. Daher</author>
    <author>C. Kästner</author>
    <author>B.-C. Krause</author>
    <author>T. Meyer</author>
    <author>P. Jalili</author>
    <author>K. Kogeveen</author>
    <author>L. Böhmert</author>
    <author>D. Lichtenstein</author>
    <author>A. Burel</author>
    <author>S. Chevance</author>
    <author>H. Jungnickel</author>
    <author>J. Tentschert</author>
    <author>P. Laux</author>
    <author>A. Braeuning</author>
    <author>F. Gauffre</author>
    <author>V. Fessard</author>
    <author>J. Meijer</author>
    <author>I. Estrela-Lopis</author>
    <author>Andreas Thünemann</author>
    <author>A. Luch</author>
    <author>A. Lampen</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Small-angle x-ray scattering</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>SAXS</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanopatricle</value>
    </subject>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>41170</id>
    <completedYear/>
    <publishedYear>2017</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>21</pageFirst>
    <pageLast>29</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>118</volume>
    <type>article</type>
    <publisherName>Elsevier</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">It takes more than a coating to get nanoparticles through the intestinal barrier in vitro</title>
    <abstract language="eng">Size and shape are crucial parameters which have impact on the potential of nanoparticles to penetrate cell membranes and epithelial barriers. Current research in nanotoxicology additionally focuses on particle coating. To distinguish between core- and coating-related effects in nanoparticle uptake and translocation, two nanoparticles equal in size, coating and charge but different in core material were investigated.&#13;
Silver and iron oxide nanoparticles coated with poly(acrylic acid) were chosen and extensively characterized by small-angle x-ray scattering, nanoparticle tracing analysis and transmission electron microscopy (TEM). Uptake and transport were studied in the intestinal Caco-2 model in a Transwell System with subsequent elemental analysis. TEM and ion beam microscopy were conducted for particle visualization.&#13;
Although equal in size, charge and coating, the behavior of the two particles in Caco-2 cells was different: while the internalized amount was comparable, only iron oxide nanoparticles additionally passed the epithelium. Our findings suggest that the coating material influenced only the uptake of the nanoparticles whereas the translocation was determined by the core material.&#13;
Knowledge about the different roles of the particle coating and core materials in crossing biological barriers will facilitate toxicological risk assessment of nanoparticles and contribute to the optimization of pharmacokinetic properties of nano-scaled pharmaceuticals.</abstract>
    <parentTitle language="eng">European Journal of Pharmaceutics and Biopharmaceutics</parentTitle>
    <identifier type="doi">10.1016/j.ejpb.2016.12.004</identifier>
    <identifier type="issn">0939-6411</identifier>
    <identifier type="issn">1873-3441</identifier>
    <enrichment key="date_peer_review">31.07.2017</enrichment>
    <author>D. Lichtenstein</author>
    <author>J. Ebmeyer</author>
    <author>T. Meyer</author>
    <author>A.-C. Behr</author>
    <author>Claudia Kästner</author>
    <author>L. Böhmert</author>
    <author>J. Juling</author>
    <author>B. Niemann</author>
    <author>C. Fahrenson</author>
    <author>S. Selve</author>
    <author>Andreas Thünemann</author>
    <author>J. Meijer</author>
    <author>I. Estrela-Lopis</author>
    <author>A. Bräuning</author>
    <author>A. Lampen</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Silver</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanoparticle</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Polymer</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Polyacrylic acid</value>
    </subject>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>40939</id>
    <completedYear/>
    <publishedYear>2017</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>S272</pageFirst>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>258</volume>
    <type>conferenceobject</type>
    <publisherName>Elsevier Ltd.</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Effects of Al-, Ti- and Zn-containing nanomaterials on cell lines in vitro</title>
    <abstract language="eng">Among the different tested endpoints, Al- and Ticontaining nanomaterials did notshowany toxicity in intestinal cell lines in vitro. Nevertheless, this absence of effect was not due to an absence of exposure, since particle-specific uptake was reported.&#13;
Metal particle uptake over a long time period might therefore be relevant for risk assessment of aluminum- and titanium-containing food products.</abstract>
    <parentTitle language="eng">TOXICOLOGY LETTERS</parentTitle>
    <identifier type="doi">10.1016/j.toxlet.2016.06.1954</identifier>
    <identifier type="issn">0378-4274</identifier>
    <enrichment key="eventName">52nd Congress of the European-Societies-of-Toxicology (EUROTOX)</enrichment>
    <enrichment key="eventPlace">Seville, Spain</enrichment>
    <enrichment key="eventStart">04.09.2017</enrichment>
    <enrichment key="eventEnd">07.09.2017</enrichment>
    <enrichment key="date_peer_review">10.07.2017</enrichment>
    <author>H. Sieg</author>
    <author>C. Lehmann</author>
    <author>Claudia Kästner</author>
    <author>B. Krause</author>
    <author>A. Burel</author>
    <author>S. Chevance</author>
    <author>L. Böhmert</author>
    <author>D. Lichtenstein</author>
    <author>J. Tentschert</author>
    <author>A. Bräuning</author>
    <author>A. Laux</author>
    <author>Andreas Thünemann</author>
    <author>I. E. Loipis</author>
    <author>V. Fessard</author>
    <author>A. Luch</author>
    <author>A. Lampen</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanoparticles</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>50632</id>
    <completedYear/>
    <publishedYear>2020</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>2246</pageFirst>
    <pageLast>2256</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>3</volume>
    <type>article</type>
    <publisherName>American Chemical Society</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Cellular Effects of In Vitro-Digested Aluminum Nanomaterials on Human Intestinal Cells</title>
    <abstract language="eng">Aluminum (Al) can be taken up from food, packaging, or the environment and thus reaches the human gastrointestinal tract. Its toxic potential after oral uptake is still discussed. The fate of different solid and ionic Al species during the passage through the digestive tract is the focus of this research, as well as the cellular effects caused by these different Al species. The present study combines the physicochemical processing of three recently studied Al species (metallic Al0, mineral Al2O3, and soluble AlCl3) in artificial digestion fluids with in vitro cell systems for the human intestinal barrier. Inductively coupled plasma mass spectrometry (ICP-MS) and small-angle X-ray scattering (SAXS) methods were used to characterize the Al species in the artificial digestion fluids and in cell culture medium for proliferating and differentiated intestinal Caco-2 cells. Cytotoxicity testing and cellular impedance measurements were applied to address the effects of digested Al species on cell viability and cell proliferation. Microarray-based transcriptome analyses and quantitative real-time PCR were conducted to obtain a deeper insight into cellular mechanisms of action and generated indications for cellular oxidative stress and an influence on xenobiotic metabolism, connected with alterations in associated signaling pathways. These cellular responses, which were predominantly caused by formerly ionic Al species and only at very high concentrations, were not impacted by artificial digestion. A two-directional conversion of Al between ionic species and solid particles occurred throughout all segments of the gastrointestinal tract, as evidenced by the presence of nanoscaled particles. Nevertheless, this presence did not increase the toxicity of the respective Al species.</abstract>
    <parentTitle language="eng">ACS Applied Nano Materials</parentTitle>
    <identifier type="doi">10.1021/acsanm.9b02354</identifier>
    <enrichment key="date_peer_review">07.05.2020</enrichment>
    <author>H. Sieg</author>
    <author>B.-C. Krause</author>
    <author>Claudia Kästner</author>
    <author>L. Böhmert</author>
    <author>D. Lichtenstein</author>
    <author>J. Tentschert</author>
    <author>H. Jungnickel</author>
    <author>P. Laux</author>
    <author>A. Braeuning</author>
    <author>V. Fessard</author>
    <author>Andreas Thünemann</author>
    <author>A. Luch</author>
    <author>A. Lampen</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>SAXS</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Small-angle X-ray scattering</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanoparticle</value>
    </subject>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="institutes" number="">6 Materialchemie</collection>
    <collection role="institutes" number="">6.5 Synthese und Streuverfahren nanostrukturierter Materialien</collection>
    <collection role="themenfelder" number="">Material</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="themenfelder" number="">Advanced Materials</collection>
  </doc>
</export-example>
