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  <doc>
    <id>53062</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>56</pageFirst>
    <pageLast>73</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>284</volume>
    <type>article</type>
    <publisherName>Wiley-Blackwell</publisherName>
    <publisherPlace>Oxford</publisherPlace>
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    <title language="eng">QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy</title>
    <abstract language="eng">A modern day light microscope has evolved from a tool devoted to making primarily empirical observations to what is now a sophisticated, quantitative device that is an integral part of both physical and life science research. Nowadays, microscopes are found in nearly every experimental laboratory. However, despite their prevalent use in capturing and quantifying scientific phenomena, neither a thorough understanding of the principles underlying quantitative imaging techniques nor appropriate knowledge of how to calibrate, operate and maintain microscopes can be taken for granted. This is clearly demonstrated by the well-documented and widespread difficulties that are routinely encountered in evaluating acquired data and reproducing scientific experiments. Indeed, studies have shown that more than 70% of researchers have tried and failed to repeat another scientist’s experiments, while more than half have even failed to reproduce their own experiments1. One factor behind the reproducibility crisis of experiments published in scientific journals is the frequent underreporting of imaging methods caused by a lack of awareness and/or a lack of knowledge of the applied technique2,3. Whereas quality control procedures for some methods used in biomedical research, such as genomics (e.g., DNA sequencing, RNA-seq) or cytometry, have been introduced (e.g. ENCODE4), this issue has not been tackled for optical microscopy instrumentation and images. Although many calibration standards and protocols have been published, there is a lack of awareness and agreement on common Standards and guidelines for quality assessment and reproducibility5.&#13;
In April 2020, the QUality Assessment and REProducibility for instruments and images in Light Microscopy (QUAREP-LiMi) initiative6 was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models7,8, and tools9,10, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research.&#13;
This White Paper 1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; 2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of Stakeholders including, researchers, imaging scientists11, bioimage analysts, bioimage informatics developers, corporate partners, Funding agencies, standards organizations, scientific publishers, and observers of such; 3) outlines the current actions of the QUAREPLiMi initiative, and 4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC.&#13;
To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics.</abstract>
    <parentTitle language="eng">Journal of microscopy</parentTitle>
    <identifier type="doi">10.1111/jmi.13041</identifier>
    <identifier type="issn">1365-2818</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-530629</identifier>
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    <enrichment key="date_peer_review">05.08.2021</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>G. Nelson</author>
    <author>U. Boehm</author>
    <author>S. Bagley</author>
    <author>P. Bajcsy</author>
    <author>J. Bischof</author>
    <author>C. M. Brown</author>
    <author>A. Dauphin</author>
    <author>I. M. Dobbie</author>
    <author>J. E. Eriksson</author>
    <author>O. Faklaris</author>
    <author>J. Fernandez-Rodriguez</author>
    <author>A. Ferrand</author>
    <author>L, Gelman</author>
    <author>A. Gheisari</author>
    <author>H. Hartmann</author>
    <author>C. Kukat</author>
    <author>A. Laude</author>
    <author>M. Mitkovski</author>
    <author>S. Munck</author>
    <author>A. J. North</author>
    <author>T. Rasse</author>
    <author>Ute Resch-Genger</author>
    <author>L. C. Schuetz</author>
    <author>A. Seitz</author>
    <author>C. Strambio-De-Castillia</author>
    <author>J. R. Swedlow</author>
    <author>I. Alexopoulos</author>
    <author>K. Aumayr</author>
    <author>S. Avilov</author>
    <author>G.-J. Bakker</author>
    <author>R. R. Bammann</author>
    <author>A. Bassi</author>
    <author>H. Beckert</author>
    <author>S. Beer</author>
    <author>Y. Belyaev</author>
    <author>J. Bierwagen</author>
    <author>K. A. Birngruber</author>
    <author>M. Bosch</author>
    <author>J. Breitlow</author>
    <author>L. A. Cameron</author>
    <author>J. Chalfoun</author>
    <author>J. J. Chambers</author>
    <author>C.-L. Chen</author>
    <author>E. Conde-Sousa</author>
    <author>A. D. Corbett</author>
    <author>F. P. Cordelieres</author>
    <author>E. Del Nery</author>
    <author>R. Dietzel</author>
    <author>F. Eismann</author>
    <author>E. Fazeli</author>
    <author>A. Felscher</author>
    <author>H. Fried</author>
    <author>N. Gaudreault</author>
    <author>W. I. Goh</author>
    <author>T. Guilbert</author>
    <author>R. Hadleigh</author>
    <author>P. Hemmerich</author>
    <author>G. A. Holst</author>
    <author>M. S. Itano</author>
    <author>C. B. Jaffe</author>
    <author>H. K. Jambor</author>
    <author>S. C. Jarvis</author>
    <author>A. Keppler</author>
    <author>D. Kirchenbuechler</author>
    <author>M. Kirchner</author>
    <author>N. Kobayashi</author>
    <author>G. Krens</author>
    <author>S. Kunis</author>
    <author>J. Lacoste</author>
    <author>M. Marcello</author>
    <author>G. G. Martins</author>
    <author>D. J. Metcalf</author>
    <author>C. A. Mitchell</author>
    <author>J. Moore</author>
    <author>T. Mueller</author>
    <author>M. S. Nelson</author>
    <author>S. Ogg</author>
    <author>S. Onami</author>
    <author>A. L. Palmer</author>
    <author>P. Paul-Gilloteaux</author>
    <author>J. A. Pimentel</author>
    <author>L. Plantard</author>
    <author>S. Podder</author>
    <author>E. Rexhepaj</author>
    <author>A. Royon</author>
    <author>M. A. Saari</author>
    <author>D. Schapman</author>
    <author>V. Schoonderwoert</author>
    <author>B. Schroth-Diez</author>
    <author>S. Schwartz</author>
    <author>M. Shaw</author>
    <author>M. Spitaler</author>
    <author>M. T. Stoeckl</author>
    <author>D. Sudar</author>
    <author>J. Teillon</author>
    <author>S. Terjung</author>
    <author>R. Thuenauer</author>
    <author>C. D. Wilms</author>
    <author>G. D. Wright</author>
    <author>R. Nitschke</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fluorescence</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Microscopy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Quality assurance</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Comparability</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Standards</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference materials</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reliability</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Data</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference data</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biology</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Medicine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Life science</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.2 Biophotonik</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/53062/jmi.13041.pdf</file>
  </doc>
  <doc>
    <id>52722</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1424</pageFirst>
    <pageLast>1427</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>18</volume>
    <type>article</type>
    <publisherName>Nature Publishing Group</publisherName>
    <publisherPlace>London</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">QUAREP-LiMi: A community endeavor to advance quality assessment and reproducibility in light microscopy</title>
    <abstract language="eng">The community-driven initiative Quality Assessment and Reproducibility for Instruments &amp; Images in Light Microscopy (QUAREP-LiMi) wants to improve reproducibility for light microscopy image data through Quality control (QC) management of instruments and images. It aims for a common set of QC guidelines for Hardware calibration and image acquisition, management and analysis.</abstract>
    <parentTitle language="eng">Nature methods</parentTitle>
    <identifier type="doi">10.1038/s41592-021-01162-y</identifier>
    <identifier type="issn">1548-7105</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">02.06.2021</enrichment>
    <enrichment key="RelatedIdentifier">https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/54270</enrichment>
    <author>U. Boehm</author>
    <author>G. Nelson</author>
    <author>C. M. Brown</author>
    <author>S. Bagley</author>
    <author>P. Bajcsy</author>
    <author>J. Bischof</author>
    <author>A. Dauphin</author>
    <author>I. M. Dobbie</author>
    <author>J. E. Eriksson</author>
    <author>O. Faklaris</author>
    <author>J. Fernandez-Rodriguez</author>
    <author>A. Ferrand</author>
    <author>L. Gelman</author>
    <author>A. Gheisari</author>
    <author>H. Hartmann</author>
    <author>C. Kukat</author>
    <author>A. Laude</author>
    <author>M. Mitkovski</author>
    <author>S. Munck</author>
    <author>A. J. North</author>
    <author>T. M. Rasse</author>
    <author>Ute Resch-Genger</author>
    <author>L. C. Schuetz</author>
    <author>A. Seitz</author>
    <author>C. Strambio-De-Castillia</author>
    <author>J. R. Swedlow</author>
    <author>R. Nitschke</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fluorescence</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Microscopy</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Quality assurance</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Comparability</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Standards</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference materials</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reliability</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Data</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Reference data</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biology</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Medicine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Life science</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.2 Biophotonik</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
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  <doc>
    <id>52432</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>7434</pageFirst>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue>20</issue>
    <volume>23</volume>
    <type>article</type>
    <publisherName>Wiley‐VCH GmbH</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
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    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A Generalized Method for High-Speed Fluorination of Metal Oxides by Spark Plasma Sintering Yields Ta3O7F and TaO2F with High Photocatalytic Activity for Oxygen Evolution from Water</title>
    <abstract language="eng">A general method to carry out the fluorination of metal oxides with poly(tetrafluoroethylene) (PTFE, Teflon) waste by spark plasma sintering (SPS) on a minute scale with Teflon is reported. The potential of this new approach is highlighted by the following results. i) The tantalum oxyfluorides Ta3O7F and TaO2F are obtained from plastic scrap without using toxic or caustic chemicals for fluorination. ii) Short reaction times (minutes rather than days) reduce the process time the energy costs by almost three orders of magnitude. iii) The oxyfluorides Ta3O7F and TaO2F are produced in gram amounts of nanoparticles. Their synthesis can be upscaled to the kg range with industrial sintering equipment. iv) SPS processing changes the catalytic properties: while conventionally prepared Ta3O7F and TaO2F show little catalytic activity, SPS-prepared Ta3O7F and TaO2F exhibit high activity for photocatalytic oxygen evolution, reaching photoconversion efficiencies up to 24.7% and applied bias to photoconversion values of 0.86%. This study shows that the materials properties are dictated by the processing which poses new challenges to understand and predict the underlying factors.</abstract>
    <parentTitle language="eng">Advanced Materials</parentTitle>
    <identifier type="doi">10.1002/adma.202007434</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-524329</identifier>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="date_peer_review">19.04.2021</enrichment>
    <licence>Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International</licence>
    <author>M. A. Lange</author>
    <author>I. Khan</author>
    <author>P. Opitz</author>
    <author>J: Hartmann</author>
    <author>M. Ashraf</author>
    <author>A. Qurashi</author>
    <author>L. Prädel</author>
    <author>M. Panthöfer</author>
    <author>Antje Cossmer</author>
    <author>Jens Pfeifer</author>
    <author>Fabian Simon</author>
    <author>Marcus von der Au</author>
    <author>Björn Meermann</author>
    <author>M. Mondeshki</author>
    <author>M. N. Tahir</author>
    <author>W. Tremel</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fluorination</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Oxygen evolution reaction</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Photocatalysis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Spark plasma sintering</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Tantalum oxyfluorides</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.1 Anorganische Spurenanalytik</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
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    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/52432/Advanced Materials 2021.pdf</file>
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    <publishedYear>2025</publishedYear>
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    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>10</pageLast>
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    <publisherName>Wiley-VCH</publisherName>
    <publisherPlace>Weinheim</publisherPlace>
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    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Ester‐Aroyl‐S,N‐Ketene Acetals with Solid‐State Luminescence: AIEgens from Sequential Three‐Component Desymmetrization</title>
    <abstract language="eng">AbstractDi(hetero)aroyl dichlorides are desymmetrized upon sequential reaction with alcohols and 2‐methyl N‐benzyl thiazolium salts within the course of a one‐pot three‐component reaction yielding ester‐substituted aroyl‐S,N‐ketene acetals under mild conditions in good yields. A prerequisite for the concise one‐pot process is the different nucleophilicity of the alcohols and in situ generated S,N‐ketene acetals. The resulting compounds are merocyanines with dominant charge‐transfer absorption bands which are fluorescent in the solid state, but not in solution. In water/ethanol solvent mixtures of increasing water content, the water‐insoluble dyes display typical aggregation‐induced emission (AIE) characteristics. The water fraction inducing AIE as well as the emission color, and fluorescence quantum yield (Φf) of the aggregated dyes can be controlled by the alcohol part of the ester moiety. Encapsulation into polystyrene nanoparticles can lead to a considerable increase of the fluorescence quantum yield Φf to 30% as shown for a representatively chosen dye revealing the highest Φf of 11% within the dye series in the water/ethanol mixtures and enabling the usage of these dyes as fluorescent reporters in aqueous environments.</abstract>
    <parentTitle language="eng">Chemistry – A European Journal</parentTitle>
    <identifier type="issn">0947-6539</identifier>
    <identifier type="doi">10.1002/chem.202502071</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-642400</identifier>
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    <enrichment key="date_peer_review">01.10.2025</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Yannic Hartmann</author>
    <author>Abdelouahad El Abbassi</author>
    <author>Bernhard Mayer</author>
    <author>Ute Resch-Genger</author>
    <author>Thomas J. J. Müller</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Dye</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fluorescence</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Aggregation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Mechanism</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Signal enhancement</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nano</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Particle</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Characterization</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Quantum yield</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Photophysics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Probe</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Sensor</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lifetime</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Polarity</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="institutes" number="">1 Analytische Chemie; Referenzmaterialien</collection>
    <collection role="institutes" number="">1.2 Biophotonik</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="themenfelder" number="">Chemie und Prozesstechnik</collection>
    <collection role="themenfelder" number="">Chemische Charakterisierung und Spurenanalytik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <collection role="themenfelder" number="">Sensorik</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/64240/Ester-Aroyl-S N-Ketene Acetals.pdf</file>
  </doc>
</export-example>
