<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>48986</id>
    <completedYear/>
    <publishedYear>2019</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>11514</pageFirst>
    <pageLast>11519</pageLast>
    <pageNumber/>
    <edition/>
    <issue>18</issue>
    <volume>91</volume>
    <type>article</type>
    <publisherName>American Chemical Society (ACS Publications)</publisherName>
    <publisherPlace>Washington</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Mass cytometry enabling absolute and fast quantiﬁcation of silver nanoparticle uptake at the single cell level</title>
    <abstract language="eng">In the last decades, signiﬁcant eﬀorts have been made to investigate possible cytotoxic eﬀects of metallic nanoparticles (NPs). Methodologies enabling precise information regarding uptake and intracellular distribution of NPs at the single cell level remain to be established. Mass cytometry (MC) has been developed for high-dimensional single cell analyses and is a promising tool to quantify NP−cell interactions. Here, we aim to establish a new MC-based quantiﬁcation procedure to receive absolute numbers of NPs per single cell by using a calibration that considers the speciﬁc transmission eﬃciency (TE) of suspended NPs. The current MC-quantiﬁcation strategy accept TE values of complementary metal solutions. In this study, we demonstrate the diﬀerent transmission behavior of 50 nm silver NPs (AgNP) and silver nitrate solution. We have used identical AgNPs for calibration as for in vitro-diﬀerentiated macrophages (THP-1 cell line) in a time- and dose-dependent manner. Our quantiﬁcation relies on silver intensities measuring AgNPs in the same detection mode as the cells. Results were comparable with the TE quantiﬁcation strategy using AgNPs but diﬀered when using ionic silver. Furthermore, intact and digested cell aliquots were measured to investigate the impact of MC sample processing on the amount of AgNPs/cell. Taken together, we have provided a MC-speciﬁc calibration procedure to precisely calculate absolute numbers of NPs per single cell. Combined with its unique feature of multiplexing up to 50 parameters, MC provides much more information on the single cell level than single cell-inductively coupled plasma mass spectrometry (SC-ICP-MS) and, therefore, oﬀers new opportunities in nanotoxicology.</abstract>
    <parentTitle language="eng">Analytical Chemistry</parentTitle>
    <identifier type="doi">10.1021/acs.analchem.9b01870</identifier>
    <identifier type="issn">0003-2700</identifier>
    <identifier type="issn">1520-6882</identifier>
    <enrichment key="date_peer_review">18.09.2019</enrichment>
    <author>Ana Lopez-Serrano Oliver</author>
    <author>A. Haase</author>
    <author>A. Peddinghaus</author>
    <author>D. Wittke</author>
    <author>Norbert Jakubowski</author>
    <author>A. Luch</author>
    <author>A. Grützkau</author>
    <author>S. Baumgart</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ICP-MS</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Nanoparticle</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Cell</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>SC-ICP-MS</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Mass cytometry</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
</export-example>
