<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>63048</id>
    <completedYear/>
    <publishedYear>2025</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>15</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>238</volume>
    <type>article</type>
    <publisherName>Elsevier Ltd.</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">The Mitsunobu reaction for the gentle covalent attachment of biomolecules to graphene oxide</title>
    <abstract language="eng">Graphene oxide (GO) has emerged as a promising biomaterial as it is easily and cheaply synthesized, strong, cytocompatible, osteoinductive, and has a well-characterized aqueous degradation pathway. It is also a great substrate for functionalization with biomolecules such as proteins, peptides, and small molecules that can enhance or add bioactivity. Covalent chemical linkages as opposed to typical noncovalent association methods are preferable so that the biomolecules do not quickly diffuse away or face replacement by other proteins, which is critical in long time scale applications like bone regeneration. However, covalent chemistry tends to carry a drawback of harsh reaction conditions that can damage the structure, conformation, and therefore function of a delicate biomolecule like a protein. Here, the Mitsunobu reaction is introduced as a novel method of covalently attaching proteins to graphene oxide. It features gentle reaction conditions and has the added benefit of utilizing the plentiful basal plane alcohol functionalities on graphene oxide, allowing for high yield protein functionalization. The amino acid Glycine (G), the protein bovine serum albumin (BSA), and the small molecule SVAK-12 are utilized to create the three Mitsunobu Graphene (MG) materials G-MG, BSA-MG, and SVAK-MG that demonstrate the wide applicability of this functionalization method.</abstract>
    <parentTitle language="eng">Carbon</parentTitle>
    <identifier type="doi">10.1016/j.carbon.2025.120221</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-630483</identifier>
    <enrichment key="opus_doi_flag">false</enrichment>
    <enrichment key="opus_import_data">&lt;div class="errorPage"&gt;    &lt;h2&gt;Anwendungsfehler&lt;/h2&gt;        &lt;div class="exceptionMessage"&gt;        Unable to Connect to ssl://api.crossref.org:443. Error #110: Connection timed out    &lt;/div&gt;        &lt;/div&gt;</enrichment>
    <enrichment key="opus.source">publish</enrichment>
    <enrichment key="opus.doi.autoCreate">false</enrichment>
    <enrichment key="opus.urn.autoCreate">true</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>M. E. Wolf</author>
    <author>W. M. Vickery</author>
    <author>W. Swift-Ramirez</author>
    <author>A. M. Arnold</author>
    <author>J. D. Orlando</author>
    <author>S. J. Schmidt</author>
    <author>Y. Liu</author>
    <author>Jasmin Er</author>
    <author>Robert Schusterbauer</author>
    <author>R. Ahmed</author>
    <author>Philip Nickl</author>
    <author>Jörg Radnik</author>
    <author>Ievgen Donskyi</author>
    <author>S. A. Sydlik</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Graphene oxide</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Mitsunobu reaction</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Covalent attachment</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Bovine serum albumin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Macrophage polarization</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Osteogenesis</value>
    </subject>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="institutes" number="">6 Materialchemie</collection>
    <collection role="institutes" number="">6.1 Oberflächen- und Dünnschichtanalyse</collection>
    <collection role="themenfelder" number="">Material</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <collection role="themenfelder" number="">Advanced Materials</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/63048/1-s2.0-S0008622325002374-main.pdf</file>
  </doc>
</export-example>
