<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>45730</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>8856</pageFirst>
    <pageLast>8864</pageLast>
    <pageNumber/>
    <edition/>
    <issue>15</issue>
    <volume>90</volume>
    <type>article</type>
    <publisherName>ACS Publ.</publisherName>
    <publisherPlace>Washington, DC</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Imaging of lipids in native human bone sections using TOF-secondary ion mass spectrometry, atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization orbitrap mass spectrometry, and orbitrap-secondary ion mass spectrometry</title>
    <abstract language="eng">A method is described for high-resolution label-free molecular imaging of human bone tissue. To preserve the lipid content and the heterogeneous structure of osseous tissue, 4 μm thick human bone sections were prepared via cryoembedding and tape-assisted cryosectioning, circumventing the application of organic solvents and a decalcification step. A protocol for comparative mass spectrometry imaging (MSI) on the same section was established for initial analysis with time-of-flight secondary ion mass spectrometry (TOF-SIMS) at a lateral resolution of 10 μm to &lt;500 nm, followed by atmospheric pressure scanning microprobe matrix-assisted laser desorption/ionization (AP-SMALDI) Orbitrap MSI at a lateral resolution of 10 μm. This procedure ultimately enabled MSI of lipids, providing the lateral localization of major lipid classes such as glycero-, glycerophospho-, and sphingolipids. Additionally, the applicability of the recently emerged Orbitrap-TOF-SIMS hybrid system was exemplarily examined and compared to the before-mentioned MSI methods.</abstract>
    <parentTitle language="eng">Analytical Chemistry</parentTitle>
    <identifier type="doi">10.1021/acs.analchem.8b00892</identifier>
    <identifier type="url">https://pubs.acs.org/doi/10.1021/acs.analchem.8b00892</identifier>
    <identifier type="issn">0003-2700</identifier>
    <identifier type="issn">1520-6882</identifier>
    <enrichment key="date_peer_review">30.08.2018</enrichment>
    <author>Kaija Schaepe</author>
    <author>D. R. Bhandari</author>
    <author>J. Werner</author>
    <author>A. Henss</author>
    <author>A. Pirkl</author>
    <author>M. Kleine-Boymann</author>
    <author>M. Rohnke</author>
    <author>S. Wenisch</author>
    <author>E. Neumann</author>
    <author>J. Janek</author>
    <author>B. Spengler</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ToF-SIMS</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>MALDI</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Mass spectrometry imaging</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lipids</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Osteoporosis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Bone</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Surface analysis</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
</export-example>
