<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>37369</id>
    <completedYear/>
    <publishedYear>2016</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1287</pageFirst>
    <pageLast>1300</pageLast>
    <pageNumber/>
    <edition/>
    <issue>9</issue>
    <volume>16</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Tuning the surface of nanoparticles: Impact of poly(2-ethyl-2-oxazoline) on protein adsorption in serum and cellular uptake</title>
    <abstract language="eng">Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach.&#13;
With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles.</abstract>
    <parentTitle language="eng">Macromolecular Bioscience</parentTitle>
    <identifier type="doi">10.1002/mabi.201600074</identifier>
    <identifier type="issn">1616-5187</identifier>
    <identifier type="issn">1616-5195</identifier>
    <enrichment key="date_peer_review">17.11.2016</enrichment>
    <author>Olga Koshkina</author>
    <author>D. Westmeier</author>
    <author>Thomas Lang</author>
    <author>C. Bantz</author>
    <author>A. Hahlbrock</author>
    <author>Christian Würth</author>
    <author>Ute Resch-Genger</author>
    <author>Ulrike Braun</author>
    <author>Raphael Thiermann</author>
    <author>C. Weise</author>
    <author>M. Eravci</author>
    <author>B. Mohr</author>
    <author>H. Schlaad</author>
    <author>R. H. Stauber</author>
    <author>D. Docter</author>
    <author>Annabelle Bertin</author>
    <author>M. Maskos</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Poloxazolines</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Protein corona</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Cellular uptake</value>
    </subject>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
</export-example>
