<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>39117</id>
    <completedYear/>
    <publishedYear>2017</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>Article e0168818, 1</pageFirst>
    <pageLast>22</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>12</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation>PLoS</creatingCorporation>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Osmoregulation in the halophilic bacterium halomonas elongata: A case study for integrative systems biology</title>
    <abstract language="eng">Halophilic bacteria use a variety of osmoregulatory methods, such as the accumulation of one or more compatible solutes. The wide diversity of compounds that can act as compatible solute complicates the task of understanding the different strategies that halophilic bacteria use to cope with salt. This is specially challenging when attempting to go beyond the pathway that produces a certain compatible solute towards an understanding of how the metabolic network as a whole addresses the problem. Metabolic reconstruction based on genomic data together with Flux Balance Analysis (FBA) is a promising tool to gain insight into this problem. However, as more of these reconstructions become available, it becomes clear that processes predicted by genome annotation may not reflect the processes that are active in vivo. As a case in point, E. coli is unable to grow aerobically on citrate in spite of having all the necessary genes to do it. It has also been shown that the realization of this genetic potential into an actual capability to metabolize citrate is an extremely unlikely event under normal evolutionary conditions. Moreover, many marine bacteria seem to have the same pathways to metabolize glucose but each species uses a different one. In this work, a metabolic network inferred from genomic annotation of the halophilic bacterium Halomonas elongata and proteomic profiling experiments are used as a starting point to motivate targeted experiments in order to find out some of the defining features of the osmoregulatory strategies of this bacterium. This new information is then used to refine the network in order to describe the actual capabilities of H. elongata, rather than its genetic potential.</abstract>
    <parentTitle language="eng">PLoS ONE</parentTitle>
    <identifier type="doi">10.1371/journal.pone.0168818</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-391172</identifier>
    <identifier type="issn">1932-6203</identifier>
    <enrichment key="date_peer_review">16.02.2017</enrichment>
    <licence>Creative Commons - Namensnennung 3.0</licence>
    <author>V. Kindzierski</author>
    <author>Silvia Raschke</author>
    <author>Nicole Knabe</author>
    <author>F. Siedler</author>
    <author>B. Scheffer</author>
    <author>K. Pflüger-Grau</author>
    <author>F. Pfeiffer</author>
    <author>D. Oesterhelt</author>
    <author>A. Marin-Sanguino</author>
    <author>Hans-Jörg Kunte</author>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Halomonas elongata</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Systems biology</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Flux balance analysis</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Proteomic analysis</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/39117/journal.pone.0168818.pdf</file>
  </doc>
  <doc>
    <id>35782</id>
    <completedYear/>
    <publishedYear>2016</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>651</pageFirst>
    <pageLast>661</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>162</volume>
    <type>article</type>
    <publisherName>SOC General Microbiology</publisherName>
    <publisherPlace>Berks, UK</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Different strategies of osmoadaptation in the closely related marine myxobacteria Enhygromyxa salina SWB007 and Plesiocystis pacifica SIR-1</title>
    <abstract language="eng">Only a few myxobacteria are known to date that are classified as marine, owing to their salt&#13;
dependency. In this study, the salt tolerance mechanism of these bacteria was investigated.&#13;
To this end, a growth medium was designed in which the mutated Escherichia coli strain&#13;
BKA13 served as sole food source for the predatory, heterotrophic myxobacteria. This enabled&#13;
measurement of the osmolytes without any background and revealed that the closely related&#13;
strains Enhygromyxa salina SWB007 and Plesiocystis pacifica SIR-1 developed different&#13;
strategies to handle salt stress. Ple. pacifica SIR-1, which was grown between 1 and 4% NaCl,&#13;
relies solely on the accumulation of amino acids, while Enh. salina SWB007, which was grown&#13;
between 0.5 and 3% NaCl, employs, besides betaine, hydroxyectoine as the major compatible&#13;
solute. In accordance with this analysis, only in the latter strain was a locus identified that codes&#13;
for genes corresponding to the biosynthesis of betaine, ectoine and hydroxyectoine.</abstract>
    <parentTitle language="eng">Microbiology</parentTitle>
    <identifier type="doi">10.1099/mic.0.000250</identifier>
    <enrichment key="date_peer_review">20.07.2016</enrichment>
    <author>J. A. Moghaddam</author>
    <author>N. Böhringer</author>
    <author>A. Burdziak</author>
    <author>Hans-Jörg Kunte</author>
    <author>E. A. Galinski</author>
    <author>T. F. Schäberle</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Osmoadaptation</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>41313</id>
    <completedYear/>
    <publishedYear>2017</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>Article 7170, 1</pageFirst>
    <pageLast>10</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>7</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Ectoine can enhance structural changes in DNA in vitro</title>
    <abstract language="eng">Strand breaks and conformational changes of DNA have consequences for the physiological role of DNA. The natural protecting molecule ectoine is beneficial to entire bacterial cells and biomolecules such as proteins by mitigating detrimental effects of environmental stresses. It was postulated that ectoine-like molecules bind to negatively charged spheres that mimic DNA surfaces. We investigated the effect of ectoine on DNA and whether ectoine is able to protect DNA from damages caused by ultraviolet radiation (UV-A). In order to determine different isoforms of DNA, agarose gel electrophoresis and atomic force microscopy experiments were carried out with plasmid pUC19 DNA. Our quantitative results revealed that a prolonged incubation of DNA with ectoine leads to an increase in transitions from supercoiled (undamaged) to open circular (single-strand break) conformation at pH 6.6. The effect is pH dependent and no significant changes were observed at physiological pH of 7.5. After UV-A irradiation in ectoine solution, changes in DNA conformation were even more pronounced and this effect was pH dependent. We hypothesize that ectoine is attracted to the negatively charge surface of DNA at lower pH and therefore fails to act as a stabilizing agent for DNA in our in vitro experiments.</abstract>
    <parentTitle language="eng">Scientific Reports</parentTitle>
    <identifier type="doi">10.1038/s41598-017-07441-z</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-413139</identifier>
    <enrichment key="date_peer_review">21.08.2017</enrichment>
    <licence>Creative Commons - Namensnennung 3.0</licence>
    <author>Susann Meyer</author>
    <author>Maria-Astrid Schröter</author>
    <author>Marc Benjamin Hahn</author>
    <author>Tihomir Solumon</author>
    <author>Heinz Strum</author>
    <author>Hans-Jörg Kunte</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ectoine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>DNA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>UV radiation</value>
    </subject>
    <collection role="ddc" number="620">Ingenieurwissenschaften und zugeordnete Tätigkeiten</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/41313/s41598-017-07441-z.pdf</file>
  </doc>
  <doc>
    <id>18560</id>
    <completedYear/>
    <publishedYear>2008</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>9475</pageFirst>
    <pageLast>9485</pageLast>
    <pageNumber/>
    <edition/>
    <issue>36</issue>
    <volume>47</volume>
    <type>article</type>
    <publisherName>ACS Publ.</publisherName>
    <publisherPlace>Washington, DC</publisherPlace>
    <creatingCorporation>American Chemical Society</creatingCorporation>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">1.55 Å Structure of the Ectoine Binding Protein TeaA of the Osmoregulated TRAP-Transporter TeaABC from Halomonas elongata</title>
    <abstract language="eng">TeaABC from the moderate halophilic bacterium Halomonas elongata belongs to the tripartite ATP-independent periplasmic transporters (TRAP-T), a family of secondary transporters functioning in conjunction with periplasmic substrate binding proteins. TeaABC facilitates the uptake of the compatible solutes ectoine and hydroxyectoine that are accumulated in the cytoplasm under hyperosmotic stress to protect the cell from dehydration. TeaABC is the only known TRAP-T activated by osmotic stress. Currently, our knowledge on the osmoregulated compatible solute transporter is limited to ABC transporters or conventional secondary transporters. Therefore, this study presents the first detailed analysis of the molecular mechanisms underlying substrate recognition of the substrate binding protein of an osmoregulated TRAP-T. In the present study we were able to demonstrate by isothermal titration calorimetry measurements that TeaA is a high-affinity ectoine binding protein (Kd = 0.19 μM) that also has a significant but somewhat lower affinity to hydroxyectoine (Kd = 3.8 μM). Furthermore, we present the structure of TeaA in complex with ectoine at a resolution of 1.55 Å and hydroxyectoine at a resolution of 1.80 Å. Analysis of the TeaA binding pocket and comparison of its structure to other compatible solute binding proteins from ABC transporters reveal common principles in compatible solute binding but also significant differences like the solvent-mediated specific binding of ectoine to TeaA.</abstract>
    <parentTitle language="eng">Biochemistry</parentTitle>
    <identifier type="old">20715</identifier>
    <identifier type="doi">10.1021/bi8006719</identifier>
    <identifier type="issn">0006-2960</identifier>
    <identifier type="issn">1520-4995</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">12.12.2008</enrichment>
    <author>S. Kuhlmann</author>
    <author>A. C. Terwisscha van Scheltinga</author>
    <author>Ralf Bienert</author>
    <author>Hans-Jörg Kunte</author>
    <author>C. M. Ziegler</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halophilic Bacteria</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halomonas elongata</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Substrate binding protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>TRAP-transporter</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>TeaABC</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>osmoregulation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>compatible solutes</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ectoine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>hydroxyectoine</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>20710</id>
    <completedYear/>
    <publishedYear>2009</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>7</pageLast>
    <pageNumber/>
    <edition/>
    <issue>#10</issue>
    <volume/>
    <type>bookpartcollection</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation>Croatian Society of Non-Destructive Testing (CrSNDT)</creatingCorporation>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Making the world a safer place - some know-how already exists</title>
    <parentTitle language="eng">MATEST 2009</parentTitle>
    <identifier type="old">23026</identifier>
    <identifier type="isbn">978-953-7283-03-2</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="eventName">MATEST 2009</enrichment>
    <enrichment key="eventPlace">Cavtat/Dubrovnik, Croatia</enrichment>
    <enrichment key="eventStart">2009-09-23</enrichment>
    <enrichment key="eventEnd">2009-09-26</enrichment>
    <author>Kurt Osterloh</author>
    <author>Norma Wrobel</author>
    <author>Hans-Jörg Kunte</author>
    <author>Uwe Zscherpel</author>
    <author>Uwe Ewert</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Existing hazards</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Threat detection</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Interdisciplinary approach</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Existing safety rules and procedures</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Bridging safety and security</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Common definitions</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>21915</id>
    <completedYear/>
    <publishedYear>2010</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>2194</pageFirst>
    <pageLast>2204</pageLast>
    <pageNumber/>
    <edition/>
    <issue>10</issue>
    <volume>49</volume>
    <type>article</type>
    <publisherName>ACS Publ.</publisherName>
    <publisherPlace>Washington, DC</publisherPlace>
    <creatingCorporation>American Chemical Society</creatingCorporation>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Structure and function of the universal stress protein TeaD and its role in regulating the ectoine transporter TeaABC of halomonas elongata DSM 2581T</title>
    <abstract language="eng">The halophilic bacterium Halomonas elongata takes up the compatible solute ectoine via the osmoregulated TRAP transporter TeaABC. A fourth orf (teaD) is located adjacent to the teaABC locus that encodes a putative universal stress protein (USP). By RT-PCR experiments we proved a cotranscription of teaD along with teaABC. Deletion of teaD resulted in an enhanced uptake for ectoine by the transporter TeaABC and hence a negative activity regulation of TeaABC by TeaD. A transcriptional regulation via DNA binding could be excluded. ATP binding to native TeaD was shown by HPLC, and the crystal structure of TeaD was solved in complex with ATP to a resolution of 1.9 Å by molecular replacement. TeaD forms a dimer–dimer complex with one ATP molecule bound to each monomer, which has a Rossmann-like α/β overall fold. Our results reveal an ATP-dependent oligomerization of TeaD, which might have a functional role in the regulatory mechanism of TeaD. USP-encoding orfs, which are located adjacent to genes encoding for TeaABC homologues, could be identified in several other organisms, and their physiological role in balancing the internal cellular ectoine pool is discussed.</abstract>
    <parentTitle language="eng">Biochemistry</parentTitle>
    <identifier type="old">24381</identifier>
    <identifier type="doi">10.1021/bi9017522</identifier>
    <identifier type="issn">0006-2960</identifier>
    <identifier type="issn">1520-4995</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">09.09.2010</enrichment>
    <author>E.S. Schweikhard</author>
    <author>S.I. Kuhlmann</author>
    <author>Hans-Jörg Kunte</author>
    <author>K. Grammann</author>
    <author>C. M. Ziegler</author>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>6294</id>
    <completedYear/>
    <publishedYear>2005</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1036</pageFirst>
    <pageLast>1043</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>187</volume>
    <type>article</type>
    <publisherName>American Society for Microbiology</publisherName>
    <publisherPlace>Washington, DC</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Potassium Transport in a Halophilic Member of the Bacteria Domain: Identification and Characterization of the K+ Uptake Systems TrkH and TrkI from Halomonas elongata DSM 2581T</title>
    <abstract language="eng">The halophilic bacterium Halomonas elongata accumulates K+, glutamate, and the compatible solute ectoine as osmoprotectants. By functional complementation of Escherichia coli mutants defective in K+ uptake, we cloned three genes that are required for K+ uptake in H. elongata. Two adjacent genes, named trkA (1,374 bp) and trkH (1,449 bp), were identified on an 8.5-kb DNA fragment, while a third gene, called trkI (1,479 bp), located at a different site in the H. elongata chromosome, was found on a second 8.5-kb fragment. The potential protein expressed by trkA is similar to the cytoplasmic NAD+/NADH binding protein TrkA from E. coli, which is required for the activity of the Trk K+ uptake system. The deduced amino acid sequences of trkH and trkI showed significant identity to the transmembrane protein of Trk transporters. K+ transport experiments with trkH and trkI mutants of H. elongata revealed that TrkI exhibits a Km value of 1.12 mM, while the TrkH system has a half-saturation constant of 3.36 mM. Strain KB12, relying on TrkH alone, accumulated K+ with a lower Vmax and required a higher K+ concentration for growth in highly saline medium than the wild type. Strain KB15, expressing only TrkI, showed the same phenotype and the same K+ transport kinetics as the wild type, proving that TrkI is the main K+ transport system in H. elongata. In the absence of both transporters TrkH and TrkI, K+ accumulation was not detectable. K+ transport was also abolished in a trkA deletion mutant, indicating that TrkI and TrkH depend on one type of TrkA protein. Reverse transcriptase PCR experiments and Northern hybridization analyses of the trkAH locus revealed cotranscription of trkAH as well as a monocistronic transcript with only trkA.</abstract>
    <parentTitle language="eng">Journal of bacteriology</parentTitle>
    <identifier type="old">6687</identifier>
    <identifier type="doi">10.1128/JB.187.3.1036-1043.2005</identifier>
    <identifier type="issn">0021-9193</identifier>
    <identifier type="issn">1098-5530</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">27.01.2005</enrichment>
    <author>A. Kraegeloh</author>
    <author>B. Amendt</author>
    <author>Hans-Jörg Kunte</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Potassium transport</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Osmoregulation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halophilic Bacteria</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halomonas elongata</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>TrkH</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>TrkA</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>11668</id>
    <completedYear/>
    <publishedYear>2005</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>289</pageFirst>
    <pageLast>300</pageLast>
    <pageNumber/>
    <edition/>
    <issue>9</issue>
    <volume/>
    <type>bookpartcollection</type>
    <publisherName>Springer</publisherName>
    <publisherPlace>Berlin</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">K+ Transport and its Role for Osmoregulation in a Halophilic Member of the Bacteria Domain: Characterization of the K+ Uptake Systems from Halomonas elongata</title>
    <parentTitle language="eng">Adaptation to life at high salt concentrations in Archaea, Bacteria, and Eukarya</parentTitle>
    <identifier type="old">13346</identifier>
    <identifier type="isbn">1-402-03632-9</identifier>
    <note>Serientitel: Cellular Origin, Life in Extreme Habitats and Astrobiology – Series title: Cellular Origin, Life in Extreme Habitats and Astrobiology</note>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <author>Hans-Jörg Kunte</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Osmoregulation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Potassium</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Glutamate</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Potassium transport</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>TrkH</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>TrkA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Trkl</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halomonas elongata</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halophilic Bacteria</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>14258</id>
    <completedYear/>
    <publishedYear>2006</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>94</pageFirst>
    <pageLast>99</pageLast>
    <pageNumber/>
    <edition/>
    <issue>2</issue>
    <volume>3</volume>
    <type>article</type>
    <publisherName>CSIRO Publishing</publisherName>
    <publisherPlace>Collingwood, Vic.</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Osmoregulation in Bacteria: Compatible Solute Accumulation and Osmosensing</title>
    <abstract language="eng">Bacteria and Archaea have developed two basic mechanisms to cope with osmotic stress, the salt-in-cytoplasm mechanism, and the organic-osmolyte mechanism. Organic osmolytes or so-called compatible solutes can be accumulated in molar concentration in the cytoplasm and allow for the adaptation of bacterial cells to varying salt concentrations. The biosynthetic pathways of compatible solutes and different compatible solute transport systems are described. A model for osmoregulatory compatible solute accumulation is introduced.</abstract>
    <parentTitle language="eng">Environmental chemistry</parentTitle>
    <identifier type="old">16040</identifier>
    <identifier type="doi">10.1071/EN06016</identifier>
    <identifier type="issn">1448-2517</identifier>
    <identifier type="issn">1449-8979</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">19.12.2006</enrichment>
    <author>Hans-Jörg Kunte</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Archaea</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Compatible solutes</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halophilic</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halotolerant</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Osmoregulated transporter</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>14156</id>
    <completedYear/>
    <publishedYear>2006</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>lecture</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Osmoregulation in halophilic bacteria: Synthesis and transport of osmoprotectants in halomonas elongata</title>
    <parentTitle language="eng">Max-Planck-Institut für Biochemie</parentTitle>
    <identifier type="old">15936</identifier>
    <enrichment key="eventName">Max-Planck-Institut für Biochemie</enrichment>
    <enrichment key="eventPlace">Planegg, Germany</enrichment>
    <enrichment key="eventStart">2006-04-26</enrichment>
    <enrichment key="eventEnd">2006-04-26</enrichment>
    <enrichment key="InvitedTalks">0</enrichment>
    <author>Hans-Jörg Kunte</author>
    <collection role="fulltextaccess" number="">Weder Datei noch physisches Exemplar vorhanden ("No Access")</collection>
    <collection role="literaturgattung" number="">Präsentation</collection>
  </doc>
  <doc>
    <id>14157</id>
    <completedYear/>
    <publishedYear>2006</publishedYear>
    <thesisYearAccepted/>
    <language>deu</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>lecture</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="deu">Mechanismen der Anpassung an Wasserstress: Synthese und Tansport von Zellschutzstoffen in halophilen Bacteria</title>
    <parentTitle language="deu">Humboldt Universität</parentTitle>
    <identifier type="old">15937</identifier>
    <enrichment key="eventName">Humboldt Universität</enrichment>
    <enrichment key="eventPlace">Berlin, Germany</enrichment>
    <enrichment key="eventStart">2006-12-19</enrichment>
    <enrichment key="eventEnd">2006-12-19</enrichment>
    <enrichment key="InvitedTalks">0</enrichment>
    <author>Hans-Jörg Kunte</author>
    <collection role="fulltextaccess" number="">Weder Datei noch physisches Exemplar vorhanden ("No Access")</collection>
    <collection role="literaturgattung" number="">Präsentation</collection>
  </doc>
  <doc>
    <id>25049</id>
    <completedYear/>
    <publishedYear>2011</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>E1285</pageFirst>
    <pageLast>E1292</pageLast>
    <pageNumber/>
    <edition/>
    <issue>49</issue>
    <volume>108</volume>
    <type>article</type>
    <publisherName>National Academy of Sciences</publisherName>
    <publisherPlace>Washington, DC</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Evidence for an allosteric mechanism of substrate release from membrane-transporter accessory binding proteins</title>
    <abstract language="eng">Numerous membrane importers rely on accessory water-soluble proteins to capture their substrates. These substrate-binding proteins (SBP) have a strong affinity for their ligands; yet, substrate release onto the low-affinity membrane transporter must occur for uptake to proceed. It is generally accepted that release is facilitated by the association of SBP and transporter, upon which the SBP adopts a conformation similar to the unliganded state, whose affinity is sufficiently reduced. Despite the appeal of this mechanism, however, direct supporting evidence is lacking. Here, we use experimental and theoretical methods to demonstrate that an allosteric mechanism of enhanced substrate release is indeed plausible. First, we report the atomic-resolution structure of apo TeaA, the SBP of the Na+-coupled ectoine TRAP transporter TeaBC from Halomonas elongata DSM2581T, and compare it with the substrate-bound structure previously reported. Conformational free-energy landscape calculations based upon molecular dynamics simulations are then used to dissect the mechanism that couples ectoine binding to structural change in TeaA. These insights allow us to design a triple mutation that biases TeaA toward apo-like conformations without directly perturbing the binding cleft, thus mimicking the influence of the membrane transporter. Calorimetric measurements demonstrate that the ectoine affinity of the conformationally biased triple mutant is 100-fold weaker than that of the wild type. By contrast, a control mutant predicted to be conformationally unbiased displays wild-type affinity. This work thus demonstrates that substrate release from SBPs onto their membrane transporters can be facilitated by the latter through a mechanism of allosteric modulation of the former.</abstract>
    <parentTitle language="eng">Proceedings of the national academy of sciences of the United States of America : PNAS</parentTitle>
    <identifier type="old">27748</identifier>
    <identifier type="doi">10.1073/pnas.1112534108</identifier>
    <identifier type="issn">0027-8424</identifier>
    <identifier type="issn">1091-6490</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">15.12.2011</enrichment>
    <author>F. Marinelli</author>
    <author>S.I. Kuhlmann</author>
    <author>E. Grell</author>
    <author>Hans-Jörg Kunte</author>
    <author>C. M. Ziegler</author>
    <author>J.D. Faraldo-Gómez</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Binding thermodynamics</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Periplasmic binding protein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Secondary transporter</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ABC transporter</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Replica-exchange metadynamics</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>28375</id>
    <completedYear/>
    <publishedYear>2013</publishedYear>
    <thesisYearAccepted/>
    <language>deu</language>
    <pageFirst/>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>poster</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="deu">How to shift and exploit the unfavourable equilibrium of ADPC formation</title>
    <parentTitle language="deu">VAAM Jahrestagung</parentTitle>
    <identifier type="old">31223</identifier>
    <enrichment key="eventName">VAAM Jahrestagung</enrichment>
    <enrichment key="eventPlace">Bremen, Germany</enrichment>
    <enrichment key="eventStart">2013-03-10</enrichment>
    <enrichment key="eventEnd">2013-03-13</enrichment>
    <author>Hans-Jörg Kunte</author>
    <collection role="fulltextaccess" number="">Weder Datei noch physisches Exemplar vorhanden ("No Access")</collection>
    <collection role="literaturgattung" number="">Präsentation</collection>
  </doc>
  <doc>
    <id>59319</id>
    <completedYear/>
    <publishedYear>2020</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>4054</pageFirst>
    <pageLast>4064</pageLast>
    <pageNumber/>
    <edition/>
    <issue>7</issue>
    <volume>45</volume>
    <type>article</type>
    <publisherName>Elsevier BV</publisherName>
    <publisherPlace>New York, NY</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Effect of microbial hydrogen consumption on the hydrogen permeation behaviour of AISI 4135 steel under cathodic protection</title>
    <abstract language="eng">The feasibility of microbial hydrogen consumption to mitigate the hydrogen embrittlement (HE) under different cathodic potentials was evaluated using the Devanathan-Stachurski electrochemical test and the hydrogen permeation efficiency h. The hydrogen permeation efficiency h in the presence of strain GA-1 was lower than that in sterile medium. The cathodic potential inhibited the adherence of strain GA-1 to AISI 4135 steel surface, thereby reducing the hydrogen consumption of strain GA-1. The adherent GA-1 cells were capable of consuming ‘cathodic hydrogen’ and reducing the proportions of absorbed hydrogen, indicating that it is theoretically possible to control HE by hydrogen-consuming microbes.</abstract>
    <parentTitle language="eng">International Journal of Hydrogen Energy</parentTitle>
    <identifier type="doi">10.1016/j.ijhydene.2019.12.058</identifier>
    <identifier type="issn">1879-3487</identifier>
    <enrichment key="opus_doi_flag">true</enrichment>
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    <title language="eng">Hydrogen release from carbon steel in chloride solution under anodic polarization</title>
    <abstract language="eng">The hydrogen permeation current increase was noticed for carbon steel in 0.5 mol/L NaCl solution under strong anodic potentials, which is contrary to the common understanding. Hydrogen permeation under cathodic potentials has been widely studied because of possible hydrogen embrittlement failures of high strength steels in seawater, but investigations of anodic polarization on hydrogen permeation are fairly rare, as the hydrogen evolution reaction shall be retarded. To corroborate the observed phenomenon, experiments were conducted using both as-received and vacuum-annealed sheet specimens. It was verified that the observed phenomena originated from the released hydrogen in traps by metal dissolution under anodic polarization.</abstract>
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    <enrichment key="InvitedTalks">0</enrichment>
    <author>Hans-Jörg Kunte</author>
    <collection role="fulltextaccess" number="">Weder Datei noch physisches Exemplar vorhanden ("No Access")</collection>
    <collection role="literaturgattung" number="">Präsentation</collection>
  </doc>
  <doc>
    <id>22344</id>
    <completedYear/>
    <publishedYear>2011</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1973</pageFirst>
    <pageLast>1994</pageLast>
    <pageNumber/>
    <edition/>
    <issue>8</issue>
    <volume>13</volume>
    <type>article</type>
    <publisherName>Blackwell Science</publisherName>
    <publisherPlace>Oxford</publisherPlace>
    <creatingCorporation>Society for Applied Microbiology</creatingCorporation>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581T</title>
    <abstract language="eng">The halophilic γ-proteobacterium Halomonas elongata DSM 2581T thrives at high salinity by synthesizing and accumulating the compatible solute ectoine. Ectoine levels are highly regulated according to external salt levels but the overall picture of its metabolism and control is not well understood. Apart from its critical role in cell adaptation to halophilic environments, ectoine can be used as a stabilizer for enzymes and as a cell protectant in skin and health care applications and is thus produced annually on a scale of tons in an industrial process using H. elongata as producer strain. This paper presents the complete genome sequence of H. elongata (4 061 296 bp) and includes experiments and analysis identifying and characterizing the entire ectoine metabolism, including a newly discovered pathway for ectoine degradation and its cyclic connection to ectoine synthesis. The degradation of ectoine (doe) proceeds via hydrolysis of ectoine (DoeA) to Nα-acetyl-L-2,4-diaminobutyric acid, followed by deacetylation to diaminobutyric acid (DoeB). In H. elongata, diaminobutyric acid can either flow off to aspartate or re-enter the ectoine synthesis pathway, forming a cycle of ectoine synthesis and degradation. Genome comparison revealed that the ectoine degradation pathway exists predominantly in non-halophilic bacteria unable to synthesize ectoine. Based on the resulting genetic and biochemical data, a metabolic flux model of ectoine metabolism was derived that can be used to understand the way H. elongata survives under varying salt stresses and that provides a basis for a model-driven improvement of industrial ectoine production.</abstract>
    <parentTitle language="eng">Environmental microbiology</parentTitle>
    <identifier type="old">24864</identifier>
    <identifier type="doi">10.1111/j.1462-2920.2010.02336.x</identifier>
    <identifier type="issn">1462-2912</identifier>
    <identifier type="issn">1462-2920</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">11.11.2010</enrichment>
    <author>Karin Schwibbert</author>
    <author>A. Marin-Sanguino</author>
    <author>I. Bagyan</author>
    <author>Gabriele Heidrich</author>
    <author>G. Lentzen</author>
    <author>H. Seitz</author>
    <author>M. Rampp</author>
    <author>S.C. Schuster</author>
    <author>H.-P. Klenk</author>
    <author>F. Pfeiffer</author>
    <author>D. Oesterheit</author>
    <author>Hans-Jörg Kunte</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Genom</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ectoin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biotechnologie</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halophile Bakterien</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>25221</id>
    <completedYear/>
    <publishedYear>2011</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>B501</pageFirst>
    <pageLast>B510</pageLast>
    <pageNumber/>
    <edition/>
    <issue>12</issue>
    <volume>13</volume>
    <type>article</type>
    <publisherName>Wiley-VCH Verl.</publisherName>
    <publisherPlace>Weinheim</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Linear and hyperbranched polyglycerol derivatives as excellent bioinert glass coating materials</title>
    <abstract language="eng">The non-specific adsorption of proteins to surfaces in contact with biofluids constitutes a major problem in the biomedical and biotechnological field, due to the initiation of biofilm formation and the resulting improper function of devices. Therefore, non-fouling surfaces modified with poly(ethylene glycol) (PEG) are usually applied. In this study, we report the synthesis of triethoxysilane modified glycerol based polymers of linear and branched architecture for the preparation of covalently attached monolayers on glass. Evaluation of the biocompatibility of these surfaces was performed in comparison to bare non-coated glass, hydrophobic hexadecane modified glass, and mPEG modified glass as the controls. Protein adsorption of BSA and fibrinogen (1 mg · mL-1 in PBS) after 4 and 24 h immersion was reduced by more than 96 and 90%, respectively, compared to the adsorption on bare glass substrates. In addition, mouse NIH-3T3 fibroblast cells showed only marginal adhesion on the polyglycerol and mPEG coated slides after 3 and 7 d incubation in cell suspension, which demonstrates the long-term stability of the applied glass coatings. The non-adhesive properties of these coatings were further reflected in bacterial adhesion tests of Escherichia coli K12 and three clinically relevant Gram-positive and negative strains (Staphylococcus aureus, Pseudomonas aeruginosa, and Aeromonas hydrophila), since linear polyglycerol (LPG(OH)), linear poly(methyl glycerol) (LPG(OMe)), and hyperbranched polyglycerol (HPG) reduced the adhesion for all tested strains by more than 99% compared to bare glass. Therefore, polyglycerol derivatives present an excellent non-fouling surface coating as an alternative to PEG with feasibility for surface modification of various substrates.</abstract>
    <parentTitle language="eng">Advanced engineering materials</parentTitle>
    <identifier type="old">27928</identifier>
    <identifier type="doi">10.1002/adem.201180012</identifier>
    <identifier type="issn">1438-1656</identifier>
    <enrichment key="date_peer_review">05.01.2012</enrichment>
    <author>M. Weinhart</author>
    <author>T. Becherer</author>
    <author>N. Schnurbusch</author>
    <author>Karin Schwibbert</author>
    <author>Hans-Jörg Kunte</author>
    <author>R. Haag</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Protein adsorption</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Non-fouling-surfaces</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Linear polyglycerol</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Hyperbranched polyglycerol</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Bacterial adhesion</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>44424</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>Article 104, 1</pageFirst>
    <pageLast>15</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>10</volume>
    <type>article</type>
    <publisherName>MDPI</publisherName>
    <publisherPlace>Basel</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Biosynthesis and characterization of zearalenone-14-sulfate, zearalenone-14-glucoside and zearalenone-16-glucoside using common fungal strains</title>
    <abstract language="eng">Zearalenone (ZEN) and its phase II sulfate and glucoside metabolites have been detected in food and feed commodities. After consumption, the conjugates can be hydrolyzed by the human intestinal microbiota leading to liberation of ZEN that implies an underestimation of the true ZEN exposure. To include ZEN conjugates in routine analysis, reliable standards are needed, which are currently not available. Thus, the aim of the present study was to develop a facilitated biosynthesis of ZEN-14-sulfate, ZEN-14-glucoside and ZEN-16-glucoside. A metabolite screening was conducted by adding ZEN to liquid fungi cultures of known ZEN conjugating Aspergillus and Rhizopus strains. Cultivation conditions and ZEN incubation time were varied. All media samples were analyzed for metabolite formation by HPLC-MS/MS. In addition, a consecutive biosynthesis was developed by using Fusarium graminearum for ZEN biosynthesis with subsequent conjugation of the toxin by utilizing Aspergillus and Rhizopus species. ZEN-14-sulfate (yield: 49%) is exclusively formed by Aspergillus oryzae. ZEN-14-glucoside (yield: 67%) and ZEN-16-glucoside (yield: 39%) are formed by Rhizopus oryzae and Rhizopus oligosporus, respectively. Purities of ≥73% ZEN-14-sulfate, ≥82% ZEN-14-glucoside and ≥50% ZEN-16-glucoside were obtained by 1H-NMR. In total, under optimized cultivation conditions, fungi can be easily utilized for a targeted and regioselective synthesis of ZEN conjugates.</abstract>
    <parentTitle language="eng">Toxins</parentTitle>
    <identifier type="doi">10.3390/toxins10030104</identifier>
    <identifier type="issn">2072-6651</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-444246</identifier>
    <enrichment key="date_peer_review">09.03.2018</enrichment>
    <licence>Creative Commons - CC BY - Namensnennung 4.0 International</licence>
    <author>Antje Borzekowski</author>
    <author>T. Drewitz</author>
    <author>Julia Keller</author>
    <author>Dietmar Pfeifer</author>
    <author>Hans-Jörg Kunte</author>
    <author>Matthias Koch</author>
    <author>S. Rohn</author>
    <author>R. Maul</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Mycotoxin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Zearalenone</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Conjugate</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biosynthesis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fusarium</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Aspergillus</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Rhizopus</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/44424/Borzekowski_Toxins_Biosynthese_2018.pdf</file>
  </doc>
  <doc>
    <id>52479</id>
    <completedYear/>
    <publishedYear>2021</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>Paper C2021-16284, 1</pageFirst>
    <pageLast/>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume/>
    <type>conferenceobject</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Microbiologically Influenced Corrosion (MIC) by Halophilic (Salt-Loving) Nitrate and Sulfate-Reducing Microorganisms</title>
    <abstract language="eng">The survey of Canadian shale sites showed a dominance of halophilic microorganisms, including Halomonas (HA). Nitrate-amended incubations of the field samples under high salinity (14.6% NaCl), revealed a dominance of HA (&gt;72%) and an accumulation of nitrite. Nitrite accumulation directly inhibited the growth of SRB, thereby decreasing their souring and corrosion risks. However, accumulated nitrite may also contribute to iron corrosion, which will be tested by using different concentrations of nitrate as an electron acceptor to HA. Different salinities are further tested on HA strains supplemented with iron coupons to determine their effects on iron corrosion rates. HA incubated with separate cultures of corrosive methanogen and SRB were tested to determine whether a positive or adverse effect will occur between them. Lastly, analyses of iron coupons will be conducted using TOF-SIMS, FIB-SEM and EDS for corrosion product characterization</abstract>
    <parentTitle language="eng">Corrosion 2021</parentTitle>
    <identifier type="url">https://my.nace.org/PaperTrail/Authors/Submission.aspx?id=2914f145-7f8f-ea11-813a-005056a95a7c</identifier>
    <enrichment key="eventName">Corrosion 2021</enrichment>
    <enrichment key="eventPlace">Online Meeting</enrichment>
    <enrichment key="eventStart">19.04.2021</enrichment>
    <enrichment key="eventEnd">30.04.2021</enrichment>
    <enrichment key="opus.source">publish</enrichment>
    <author>Biwen Annie An</author>
    <author>Hans-Jörg Kunte</author>
    <author>Andrea Koerdt</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>MIC</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Bacteria</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Halophile</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Corrosion</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Environmental condition</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Korrosion</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>High salinity</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="institutes" number="">4 Material und Umwelt</collection>
    <collection role="institutes" number="">4.1 Biologische Materialschädigung und Referenzorganismen</collection>
    <collection role="themenfelder" number="">Umwelt</collection>
    <collection role="themenfelder" number="">Umwelt-Material-Interaktionen</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="literaturgattung" number="">Graue Literatur</collection>
  </doc>
  <doc>
    <id>31636</id>
    <completedYear/>
    <publishedYear>2014</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>124</pageFirst>
    <pageLast>130</pageLast>
    <pageNumber/>
    <edition/>
    <issue>1</issue>
    <volume>359</volume>
    <type>article</type>
    <publisherName>Wiley-Blackwell</publisherName>
    <publisherPlace>Malden, Mass., USA</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Biotransformation of the mycotoxin zearalenone by fungi of the genera Rhizopus and Aspergillus</title>
    <abstract language="eng">Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin biosynthesized by various Fusarium fungi. These fungal species frequently infest grains; therefore, ZEN represents a common contaminant in cereal products. The biotransformation of ZEN differs significantly from species to species, and several metabolites are known to be formed by animals, plants, and microorganisms. The aim of the present study was to investigate the microbial conversion of ZEN by species of the genera Rhizopus and Aspergillus representing relevant fungi for food processing (e.g. fermentation). To monitor the ZEN metabolism, ZEN was added to liquid cultures of the different fungal species. After a period of 3 days, the media were analyzed by HPLC-MS/MS for metabolite formation. Two Aspergillus oryzae strains and all seven Rhizopus species were able to convert ZEN into various metabolites, including ZEN-14-sulfate as well as ZEN-O-14- and ZEN-O-16-glucoside. Microbial transformation of ZEN into the significantly more estrogenic α-zearalenol (α-ZEL) was also observed. Additionally, a novel fungal metabolite, α-ZEL-sulfate, was detected. Semi-quantification of the main metabolites indicates that more than 50% of initial ZEN may be modified. The results show that fungal strains have the potential to convert ZEN into various metabolites leading to a masking of the toxin, for example in fermented food.</abstract>
    <parentTitle language="eng">FEMS microbiology letters</parentTitle>
    <identifier type="old">34665</identifier>
    <identifier type="doi">10.1111/1574-6968.12586</identifier>
    <identifier type="issn">0378-1097</identifier>
    <identifier type="issn">1574-6968</identifier>
    <enrichment key="date_peer_review">16.10.2014</enrichment>
    <author>Antje Brodehl</author>
    <author>Anne Möller</author>
    <author>Hans-Jörg Kunte</author>
    <author>Matthias Koch</author>
    <author>Ronald Maul</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Microbial conversion</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Metabolites</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Fermentation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Alpha-zearalenol</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Conjugation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Mycotoxin biotransformation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Zearalenone-sulfate</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>41164</id>
    <completedYear/>
    <publishedYear>2017</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1</pageFirst>
    <pageLast>5</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>14</volume>
    <type>conferenceobject</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation>International Raditation Protection Association</creatingCorporation>
    <contributingCorporation/>
    <belongsToBibliography>0</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Development of a standard procedure for the irradiation of biomolecules</title>
    <abstract language="eng">In dosimetry the determination of the effectiveness of the damaging processes is standardized and accounted for by the radiation and tissue weighting factor. For the underlying constituents of the tissue, that is the various biomolecules, such a systematic approach doesn't exist. This makes it difficult to compare results obtained under different experimental conditions. In the following work, we will describe a method to obtain comparable values for the radiation-biomolecule interaction, measured under different conditions. This approach can lead to standardization of dosedamage relationship at the molecular level. Such approach is necessary for a better understanding of the relations between the damage of the single constituents of biological tissue and the whole – finally gaining a more complete picture of irradiation damage.</abstract>
    <parentTitle language="eng">Proceedings of the 14th IRPA  International Congress</parentTitle>
    <enrichment key="eventName">14th International Congress of the International Raditation Protection Association (IRPA)</enrichment>
    <enrichment key="eventPlace">Cape Town, South Africa</enrichment>
    <enrichment key="eventStart">09.05.2016</enrichment>
    <enrichment key="eventEnd">12.05.2016</enrichment>
    <author>Marc Benjamin Hahn</author>
    <author>Tihomir Solomun</author>
    <author>Susann Meyer</author>
    <author>Hans-Jörg Kunte</author>
    <author>Maria-Astrid Schröter</author>
    <author>Heinz Sturm</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Dosimetry</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
    <collection role="literaturgattung" number="">Graue Literatur</collection>
  </doc>
  <doc>
    <id>38698</id>
    <completedYear/>
    <publishedYear>2017</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1798</pageFirst>
    <pageLast>1805</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>19</volume>
    <type>article</type>
    <publisherName>Royal Society of Chemistry</publisherName>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Direct electron irradiation of DNA in fully aqueous environment. Damage determination in combination with Monte Carlo simulations</title>
    <abstract language="eng">We report on a study in which plasmid DNA in water was irradiated with 30 keV electrons generated by a scanning electron microscope and passed through a 100 nm thick Si3N4 membrane. The corresponding Monte Carlo simulations suggest that the kinetic energy spectrum of the electrons throughout the water is dominated by low energy electrons (&lt;100 eV). The DNA radiation damage, single-strand breaks (SSB) and double-strand breaks (DSB), was determined by electrophoresis. The median lethal dose of D1/2 = 1.7 ± 0.3 Gy was found to be much smaller compared to partially or fully hydrated DNA irradiated under vacuum conditions. The ratio of DSB to SSB was found to be (1:12) as compared to 1:88) found for hydrated DNA. Our method enables quantitative measurements of radiation damage to biomolecules (DNA, proteins) in solutions under varying conditions (pH, salinity, cosolutes) for an electron energy range which is difficult to probe by standard methods.</abstract>
    <parentTitle language="eng">Physical Chemistry Chemical Physics</parentTitle>
    <identifier type="doi">10.1039/C6CP07707B</identifier>
    <identifier type="issn">1463-9076</identifier>
    <identifier type="issn">1463-9084</identifier>
    <identifier type="urn">urn:nbn:de:kobv:b43-386981</identifier>
    <enrichment key="date_peer_review">20.04.2017</enrichment>
    <enrichment key="PaperofMonth">1</enrichment>
    <licence>Creative Commons - Namensnennung 3.0</licence>
    <author>Marc Benjamin Hahn</author>
    <author>Susann Meyer</author>
    <author>Maria-Astrid Schröter</author>
    <author>H. Seitz</author>
    <author>Hans-Jörg Kunte</author>
    <author>Tihomir Solomun</author>
    <author>Heinz Sturm</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Plasmid DNA in water</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Monte Carlo simulation</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Low energy electrons</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>DNA radiation damage</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Single-strand break (SSB)</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Double-strand break (DSB)</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Lethal dose</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Radiation damage to biomolecules</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Solutions (pH, salinity, cosolutes)</value>
    </subject>
    <collection role="ddc" number="543">Analytische Chemie</collection>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei für die Öffentlichkeit verfügbar ("Open Access")</collection>
    <collection role="unnumberedseries" number="">Wissenschaftliche Artikel der BAM</collection>
    <thesisPublisher>Bundesanstalt für Materialforschung und -prüfung (BAM)</thesisPublisher>
    <file>https://opus4.kobv.de/opus4-bam/files/38698/Hahn_2016_Direct electron irradiation of DNA.pdf</file>
  </doc>
</export-example>
