30126
2014
eng
45
52
1
7
article
Wageningen Academic Publishers
Wageningen
1
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--
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Preparation of 13C-labelled cis-zearalenone and its application as internal standard in stable isotope dilution analysis
Pure U-[13C18]-labelled cis-zearalenone (cis-ZEA) has been prepared and characterised as internal standard (ISTD) for a reliable quantification of cis-ZEA in contaminated food and feed products. The cis-isomer of the naturally trans-configurated Fusarium mycotoxin zearalenone is often neglected. However, isomerisation easily occurs by exposure of ZEA to (UV-)light. Thus, the applicability of the new cis-ZEA ISTD was demonstrated in a long-term isomerisation study comparing naturally trans-ZEA-contaminated edible oil with spiked edible oil. To estimate the benefits of the newly prepared cis-ZEA ISTD, various approaches to quantify cis-ZEA by high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) were compared. As a result, a significant bias was revealed if no appropriate cis-ZEA standards are used. Furthermore, the new ISTD was applied to the analysis of 15 edible oils by stable isotope dilution analysis in combination with HPLC-electrospray ionisation-MS/MS. One of the maize germ oils showed the presence of cis-ZEA above LOD (>0.3 µg/kg), whereas two out of 15 maize germ oils were found to be contaminated with trans-ZEA (range 17.0-31.0 µg/kg).
World mycotoxin journal
33076
10.3920/WMJ2013.1610
1875-0710
1875-0796
10.02.2014
Sarah Drzymala
Juliane Riedel
Robert Köppen
L.-A. Garbe
Matthias Koch
eng
uncontrolled
ZEA
eng
uncontrolled
Zearalenone
eng
uncontrolled
Isomerisation
eng
uncontrolled
Occurrence
eng
uncontrolled
Edible oil
eng
uncontrolled
SIDA
eng
uncontrolled
HPLC-MS/MS
eng
uncontrolled
Quantification
eng
uncontrolled
LC-MS/MS
Verlagsliteratur
Datei im Netzwerk der BAM verfügbar ("Closed Access")
33067
2015
eng
3489
3497
12
407
article
Springer
Berlin
1
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Automated solid-phase extraction coupled online with HPLC-FLD for the quantification of zearalenone in edible oil
Established maximum levels for the mycotoxin zearalenone (ZEN) in edible oil require monitoring by reliable analytical methods. Therefore, an automated SPE-HPLC online system based on dynamic covalent hydrazine chemistry has been developed. The SPE step comprises a reversible hydrazone formation by ZEN and a hydrazine moiety covalently attached to a solid phase. Seven hydrazine materials with different properties regarding the resin backbone, pore size, particle size, specific surface area, and loading have been evaluated. As a result, a hydrazine-functionalized silica gel was chosen. The final automated online method was validated and applied to the analysis of three maize germ oil samples including a provisionally certified reference material. Important performance criteria for the recovery (70–120 %) and precision (RSDr <25 %) as set by the Commission Regulation EC 401/2006 were fulfilled: The mean recovery was 78 % and RSDr did not exceed 8 %. The results of the SPE-HPLC online method were further compared to results obtained by liquid&–liquid extraction with stable isotope dilution analysis LC-MS/MS and found to be in good agreement. The developed SPE-HPLC online system with fluorescence detection allows a reliable, accurate, and sensitive quantification (limit of quantification, 30 µg/kg) of ZEN in edible oils while significantly reducing the workload. To our knowledge, this is the first report on an automated SPE-HPLC method based on a covalent SPE approach.
Analytical and bioanalytical chemistry
36146
10.1007/s00216-015-8541-5
1618-2642
1618-2650
22.04.2015
Sarah Drzymala
S. Weiz
J. Heinze
S. Marten
Carsten Prinz
Annett Zimathies
L.-A. Garbe
Matthias Koch
eng
uncontrolled
Dynamic covalent hydrazine chemistry (DCHC)
eng
uncontrolled
Maize germ oil
eng
uncontrolled
Food
eng
uncontrolled
Mycotoxin
eng
uncontrolled
ZEA
eng
uncontrolled
Isomerisation
eng
uncontrolled
Occurrence
eng
uncontrolled
Edible oil
eng
uncontrolled
SIDA
eng
uncontrolled
LC-MS/MS
eng
uncontrolled
Quantification
Verlagsliteratur
Datei im Netzwerk der BAM verfügbar ("Closed Access")