<?xml version="1.0" encoding="utf-8"?>
<export-example>
  <doc>
    <id>30450</id>
    <completedYear/>
    <publishedYear>2014</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>227</pageFirst>
    <pageLast>232</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>479-480</volume>
    <type>article</type>
    <publisherName>Elsevier B.V.</publisherName>
    <publisherPlace>Amsterdam</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Evaluation of the anthropogenic input of caffeine in surface waters of the north and center of Portugal by ELISA</title>
    <abstract language="eng">This study comprises the development of an enzyme-linked immunosorbent assay (ELISA) for the quantification of caffeine in complex aqueous matrices without any sample clean-up procedure. Salinity and dissolved organic matter were selected as potential interfering agents. The addition of a sample buffer containing bovine serum albumin (BSA) prior to the sample was found to decrease the influence of those interfering agents. The working range of the developed method was 0.1–100 µg L- 1. Quantification of caffeine was possible in 43 out of 51 real aqueous samples, at values between &lt; LOD and 15 µg L- 1. Results correlate well with those obtained by LCMS/MS. To the best of our knowledge this is the first study dealing with the quantification of caffeine in Portugal's surface waters.</abstract>
    <parentTitle language="eng">The science of the total environment</parentTitle>
    <identifier type="old">33423</identifier>
    <identifier type="doi">10.1016/j.scitotenv.2014.01.120</identifier>
    <identifier type="issn">0048-9697</identifier>
    <enrichment key="date_peer_review">31.03.2014</enrichment>
    <author>C.P. Silva</author>
    <author>D.L.D. Lima</author>
    <author>Rudolf Schneider</author>
    <author>M. Otero</author>
    <author>V.I. Esteves</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Caffeine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Surface water</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ELISA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Anthropogenic markers</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Koffein</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Eintrag</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Oberflächenwasser</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Gewässer</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Abwasser</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Antikörper</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>35289</id>
    <completedYear/>
    <publishedYear>2016</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>513</pageFirst>
    <pageLast>527</pageLast>
    <pageNumber/>
    <edition/>
    <issue>2</issue>
    <volume>64</volume>
    <type>article</type>
    <publisherName>American Chemical Society</publisherName>
    <publisherPlace>Columbus, Ohio</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Towards the standardization of biochar analysis: the COST action TD1107 interlaboratory comparison</title>
    <abstract language="eng">Biochar produced by pyrolysis of organic residues is increasingly used for soil amendment and many other applications. However, analytical methods for its physical and chemical characterization are yet far from being specifically adapted, optimized, and standardized. Therefore, COST Action TD1107 conducted an interlaboratory comparison in which 22 laboratories from 12 countries analyzed three different types of biochar for 38 physical–chemical parameters (macro- and microelements, heavy metals, polycyclic aromatic hydrocarbons, pH, electrical conductivity, and specific surface area) with their preferential methods. The data were evaluated in detail using professional interlaboratory testing software. Whereas intralaboratory repeatability was generally good or at least acceptable, interlaboratory reproducibility was mostly not (20% &lt; mean reproducibility standard deviation &lt; 460%). This paper contributes to better comparability of biochar data published already and provides recommendations to improve and harmonize specific methods for biochar analysis in the future.</abstract>
    <parentTitle language="eng">Journal of agricultural and food chemistry</parentTitle>
    <identifier type="doi">10.1021/acs.jafc.5b05055</identifier>
    <identifier type="issn">0021-8561</identifier>
    <identifier type="issn">1520-5118</identifier>
    <enrichment key="date_peer_review">26.01.2016</enrichment>
    <author>H. J. Bachmann</author>
    <author>T. D. Bucheli</author>
    <author>A. Dieguez-Alonso</author>
    <author>D. Fabbri</author>
    <author>H. Knicker</author>
    <author>H.-P. Schmidt</author>
    <author>A. Ulbricht</author>
    <author>Roland Becker</author>
    <author>A. Buscaroli</author>
    <author>D. Buerge</author>
    <author>A. Cross</author>
    <author>D. Dickinson</author>
    <author>A. Enders</author>
    <author>V.I. Esteves</author>
    <author>M. W. H. Evangelou</author>
    <author>G. Fellet</author>
    <author>K. Friedrich</author>
    <author>G. Gasco Guerrero</author>
    <author>B. Glaser</author>
    <author>U. M. Hanke</author>
    <author>K. Hanley</author>
    <author>I. Hilber</author>
    <author>D. Kalderis</author>
    <author>J. Leifeld</author>
    <author>O. Masek</author>
    <author>J. Mumme</author>
    <author>M. Paneque Carmona</author>
    <author>R. Calvelo Pereira</author>
    <author>F. Rees</author>
    <author>A. G. Rombola</author>
    <author>J. M. de la Rosa</author>
    <author>R. Sakrabani</author>
    <author>S. Sohi</author>
    <author>G. Soja</author>
    <author>M. Valagussa</author>
    <author>F. Verheijen</author>
    <author>F. Zehetner</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biochar</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Analysis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Standardization</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ring test</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Interlaboratory comparison</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>33818</id>
    <completedYear/>
    <publishedYear>2015</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>205</pageFirst>
    <pageLast>214</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>202</volume>
    <type>article</type>
    <publisherName>Elsevier</publisherName>
    <publisherPlace>New York, NY [u.a.]</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">How life history influences the responses of the clam Scrobicularia plana to the combined impacts of carbamazepine and pH decrease</title>
    <abstract language="eng">In the present study, the bivalve Scrobicularia plana, collected from two contrasting areas (pristine location and mercury contaminated area), was selected to assess the biochemical alterations imposed by pH decrease, carbamazepine (an antiepileptic) and the combined effect of both stressors. The effects on oxidative stress related biomarkers after 96 h exposure revealed that pH decrease and carbamazepine induced alterations on clams, with greater impacts on individuals from the contaminated area which presented higher mortality, higher lipid peroxidation and higher glutathione S-transferase activity. These results emphasize the risk of extrapolating results from one area to another, since the same species inhabiting different areas may be affected differently when exposed to the same stressors. Furthermore, the results obtained showed that, when combined, the impact of pH decrease and carbamazepine was lower than each stressor acting alone, which could be related to the defence mechanism of valves closure when bivalves are under higher stressful conditions.</abstract>
    <parentTitle language="eng">Environmental pollution</parentTitle>
    <identifier type="old">36939</identifier>
    <identifier type="doi">10.1016/j.envpol.2015.03.023</identifier>
    <identifier type="issn">0269-7491</identifier>
    <identifier type="issn">0013-9327</identifier>
    <identifier type="issn">1873-6424</identifier>
    <enrichment key="date_peer_review">20.08.2015</enrichment>
    <author>R. Freitas</author>
    <author>Ângela Almeida</author>
    <author>V. Calisto</author>
    <author>C. Velez</author>
    <author>A. Moreira</author>
    <author>Rudolf Schneider</author>
    <author>V.I. Esteves</author>
    <author>F. J. Wrona</author>
    <author>A.M.V.M. Soares</author>
    <author>E. Figueira</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Ocean acidification</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biomarkers</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Oxidative stress</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Bivalves</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Pharmaceutical drugs</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>30614</id>
    <completedYear/>
    <publishedYear>2014</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>102</pageFirst>
    <pageLast>106</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>125</volume>
    <type>article</type>
    <publisherName>Elsevier</publisherName>
    <publisherPlace>Amsterdam</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Application of dispersive liquid-liquid microextraction for estrogens' quantification by enzyme-linked immunosorbent assay</title>
    <abstract language="eng">Estrogens, such as 17β-estradiol (E2) and 17α-ethinylestradiol (EE2), are the major responsible for endocrine-disrupting effects observed in aquatic environments due to their high estrogenic potency, even at concentrations ranging from pg L-1 to ng L-1. Thus, it is essential to develop analytical methodologies suitable for monitoring their presence in water samples. Dispersive liquidliquid microextraction (DLLME) was used as a pre-concentration step prior to the quantification of E2 and EE2 by enzyme-linked immunosorbent assay (ELISA). First, an evaluation of the effect of DDLME on the E2 and EE2 ELISA calibration curves was performed. Since the extraction procedure itself had an influence on the ELISA optical density (OD), it became necessary to subject, not only the samples, but also all the standards to the DLLME process. Working ranges were determined, being between 1.2 and 8000 ng L-1, for E2, and between 0.22 and 1500 ng L-1, for EE2. The influence of organic matter, both in the extraction and quantification, was evaluated and it was observed that its presence in the solution did not affect considerably the calibration curve. Recovery rates were also determined, ranging from 77% to 106% for ultrapure water and from 104% to 115% for waste water samples, the most complex ones in what concerns matrix effects. Results obtained when applying the proposed method to real water samples can be considered quite satisfying. Moreover, the obtained working ranges encompass values generally reported in literature, confirming the practical use of the method for environmental samples.</abstract>
    <parentTitle language="eng">Talanta</parentTitle>
    <identifier type="old">33590</identifier>
    <identifier type="doi">10.1016/j.talanta.2014.02.069</identifier>
    <identifier type="issn">0039-9140</identifier>
    <enrichment key="date_peer_review">28.04.2014</enrichment>
    <author>D.L.D. Lima</author>
    <author>C.P. Silva</author>
    <author>Rudolf Schneider</author>
    <author>M. Otero</author>
    <author>V.I. Esteves</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>DLLME</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ELISA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Water samples</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Environment</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>17beta-estradiol</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>17alpha-ethinylestradiol</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>43297</id>
    <completedYear/>
    <publishedYear>2017</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1129</pageFirst>
    <pageLast>1145</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>580</volume>
    <type>article</type>
    <publisherName/>
    <publisherPlace/>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Toxicity associated to uptake and depuration of carbamazepine in the clam Scrobicularia plana under a chronic exposure</title>
    <abstract language="eng">Carbamazepine (CBZ) is an antiepileptic drug commonly detected in aquatic systems, with toxic effects to inhabiting organisms. Limited information is known on stress response biomarkers associated to bioconcentration and depuration of CBZ in aquatic organisms. Moreover, few studies addressed if the response and recovery of organisms to a contaminant can change when they are collected in a contaminated site. This study intended to understand the bioconcentration and depuration of CBZ combined with its toxicological impact in Scrobicularia plana clams collected from two contrasting areas (MIRA, Mira channel, non-contaminated and LAR, Laranjo bay, arithropogenically impacted) from the Ria de Aveiro (Portugal). The clams were exposed for 14 days to environmentally relevant CBZ concentrations (0.0, 4.0 and 8.0 mu g/L), followed by a 14 day depuration period. CBZ concentrations in S. plana tissues were rapidly bioconcentrated during the exposure period. In the depuration period CBZ was eliminated, in some extent. The main toxic effects occurred at the highest concentration (8.0 mu g/L) after 14 days of exposure in which the clams from LAR accumulated ahigher CBZ concentration (LAR: similar to 10 ng/g FW) than clams from MIRA (MIRA: similar to 7 ng/g FW). LAR clams exhibited higher oxidative damage at this concentration, demonstrated by higher LPO levels over time (increase of similar to 1.4% relative to control) and, in comparison with MIRA clams (LAR: 17.7 nmol/g FW; MIRA: 11.4 nmol/g FW). After the depuration period, LAR clams recovered from the stress induced by CBZ. A decrease in LPO for LAR (decrease of similar to 40% in relation to the end of the exposure period) was accompanied by a decrease in CBZ tissue concentrations (decrease of similar to 61% relative to the end of the exposure period). MIRA clams were not oxidatively injured (low LPO levels remained unchanged after the depuration and CBZ decreased similar to 80% relative to the end of the exposure period).</abstract>
    <parentTitle language="eng">SCIENCE OF THE TOTAL ENVIRONMENT</parentTitle>
    <identifier type="doi">10.1016/j.scitotenv.2016.12.069</identifier>
    <identifier type="issn">0048-9697</identifier>
    <enrichment key="date_peer_review">05.12.2017</enrichment>
    <author>Rudolf Schneider</author>
    <author>Ângela Almeida</author>
    <author>V. Calisto</author>
    <author>V.I. Esteves</author>
    <author>A.M.V.M. Soares</author>
    <author>E. Figueira</author>
    <author>R. Freitas</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Invertebrates</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Pharmaceutical drugs</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biomarkers</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Oxidative stress</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>44902</id>
    <completedYear/>
    <publishedYear>2018</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>10</pageFirst>
    <pageLast>19</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>198</volume>
    <type>article</type>
    <publisherName>Elsevier</publisherName>
    <publisherPlace>Amsterdam, NL</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Effects of single and combined exposure of pharmaceutical drugs (carbamazepine and cetirizine) and a metal (cadmium) on the biochemical responses of R. philippinarum</title>
    <abstract language="eng">In the aquatic environment, organisms are exposed to complex mixtures of contaminants which may alter the toxicity profile of each compound, compared to its toxicity alone. Pharmaceutical drugs (e.g. carbamazepine (CBZ) and cetirizine (CTZ)) and metals (e.g. cadmium (Cd)) are among those contaminants that co-occur in the environment. However, most studies concerning their toxicity towards aquatic species are based on single exposure experiments. Thus, the present study aimed to evaluate single and combined effects of Cd and CBZ or CTZ (single conditions: Cd, CTZ, CBZ; combined conditions: CTZ+Cd, CBZ+Cd) on biomarkers related to oxidative stress and energy metabolism in the edible clam Ruditapes philippinarum, by exposing the organisms for 28 days to environmentally relevant concentrations of these contaminants. The biomarkers studied were: i) the electron transport system activity, protein and glycogen contents (indicators of organisms’ metabolic status and energy reserves); ii) lipid peroxidation and the ratio between reduced and oxidized glutathione (indicators of oxidative stress); iii) superoxide dismutase and catalase activities (enzymes indicators of antioxidant defence) and iv) activity of glutathione S-transferases (family of enzymes indicators of biotransformation capacity). Results obtained showed that the uptake of Cd and CBZ was not affected by the combined presence of the contaminants.&#13;
However, for CTZ, the uptake was higher in the presence than in the absence of Cd. Concerning toxicity data, in general, the combined exposures (CTZ+Cd, CBZ+Cd) had lower biological effects than the contaminants alone. Nevertheless, our data showed that despite the low concentrations tested, they were enough to exert biological effects that differed between single and combined treatments, evidencing the need to conduct more co-exposure studies to increase the environmental relevance of the gathered data.</abstract>
    <parentTitle language="eng">Aquatic Toxicology</parentTitle>
    <identifier type="doi">10.1016/j.aquatox.2018.02.011</identifier>
    <identifier type="issn">0166-445X</identifier>
    <identifier type="issn">1879-1514</identifier>
    <enrichment key="date_peer_review">16.05.2018</enrichment>
    <author>Â. Almeida</author>
    <author>V. Calisto</author>
    <author>V.I. Esteves</author>
    <author>Rudolf Schneider</author>
    <author>A.M.V.M. Soares</author>
    <author>E. Figueira</author>
    <author>R. Freitas</author>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Biomarker</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Arzneimittel</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Metalle</value>
    </subject>
    <subject>
      <language>deu</language>
      <type>uncontrolled</type>
      <value>Invertebraten</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ELISA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Carbamazepine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Cetirizine</value>
    </subject>
    <collection role="ddc" number="628">Sanitär- und Kommunaltechnik; Umwelttechnik</collection>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>31336</id>
    <completedYear/>
    <publishedYear>2014</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>74</pageFirst>
    <pageLast>87</pageLast>
    <pageNumber/>
    <edition/>
    <issue/>
    <volume>156</volume>
    <type>article</type>
    <publisherName>Elsevier</publisherName>
    <publisherPlace>Amsterdam [u.a.]</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Presence of the pharmaceutical drug carbamazepine in coastal systems: Effects on bivalves</title>
    <abstract language="eng">Carbamazepine (CBZ), an antiepileptic drug, is one of the most commonly detected pharmaceutical drugs in aquatic ecosystems, and is used as a marker of urban pollution. Since CBZ is designed to exert a biological effect, when it reaches aquatic environment high probability exist for toxic effects on non-target organisms. The present study evaluated the acute toxicity of environmentally relevant concentrations of CBZ (0.00, 0.03, 0.30, 3.00, 9.00 µg/L) in the edible clams Venerupis decussata (a native species) and Venerupis philippinarum (an invasive species) collected from the Ria de Aveiro. The effects on both species were assessed through the use of a battery of biomarkers mainly related with health status and oxidative stress. Furthermore, in this work an alternative and promising tool, the direct competitive immunoassay ELISA, for the direct CBZ quantification in clam's tissues, was applied.&#13;
&#13;
The results of the present work showed that CBZ in clam's tissues increased with the exposure concentration and V. decussata gave slightly higher values than V. philippinarum. Although the clams accumulated lower levels of CBZ than the concentration of exposure, these concentrations were enough to impair the health status and induce oxidative stress. However, a different response to CBZ was observed in the two species. While in V. philippinarum the lipid peroxidation levels increased at the highest CBZ concentration (9.00 µg/L), in V. decussata a significant decrease was seen. Moreover, glutathionse S-transferase activity was stimulated in V. decussata and decreased in V. philippinarum. Nevertheless, an induction of glutathione reductase, superoxide dismutase and cytochrome P450 3A4 activities was found in both species as a result of the exposure. The results indicate that, probably, V. philippinarum have a less efficient antioxidant system than V. decussata, and are therefore less capable to neutralize oxidative stress and consequently more sensitive to CBZ. The risk quotient determined for the Ria de Aveiro was higher than 1 indicating that a ecotoxicological risk is suspected. Furthermore, bioaccumulation of CBZ in clams should be taken into consideration since this chemical might be transferred along the food chain and affect non-target organisms.</abstract>
    <parentTitle language="eng">Aquatic toxicology</parentTitle>
    <identifier type="old">34347</identifier>
    <identifier type="doi">10.1016/j.aquatox.2014.08.002</identifier>
    <identifier type="issn">0166-445x</identifier>
    <identifier type="issn">1879-1514</identifier>
    <enrichment key="date_peer_review">11.09.2014</enrichment>
    <author>Ângela Almeida</author>
    <author>V. Calisto</author>
    <author>V.I. Esteves</author>
    <author>Rudolf Schneider</author>
    <author>A.M.V.M. Soares</author>
    <author>E. Figueira</author>
    <author>R. Freitas</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Acute toxicity</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Biomarkers</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ELISA immunoassay</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Pharmaceuticals</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Clams</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Carbamazepin</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Toxikologie</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Toxicology</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>21108</id>
    <completedYear/>
    <publishedYear>2010</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>3101</pageFirst>
    <pageLast>3106</pageLast>
    <pageNumber/>
    <edition/>
    <issue>5</issue>
    <volume>58</volume>
    <type>article</type>
    <publisherName>American Chemical Society</publisherName>
    <publisherPlace>Columbus, Ohio</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Sorption-desorption behavior of atrazine on soils subjected to different organic long-term amendments</title>
    <abstract language="eng">Sorption of atrazine on soils subjected to three different organic amendments was measured using a batch equilibrium technique. A higher KF value (2.20 kg-1(mg L-1)-N) was obtained for soil fertilized with compost, which had a higher organic matter (OM) content. A correlation between the KFOC values and the percentage of aromatic carbon in OM was observed. The highest KFOC value was obtained for the soil with the highest aromatic content. Higher aromatic content results in higher hydrophobicity of OM, and hydrophobic interactions play a key role in binding of atrazine. On the other hand, the soil amended with farmyard manure had a higher content of carboxylic units, which could be responsible for hydrogen bonding between atrazine and OM. Dominance of hydrogen bonds compared to hydrophobic interactions can be responsible for the lower desorption capacity observed with the farmyard manure soil. The stronger hydrogen bonding can reduce the leaching of atrazine into drinking water resources and runoff to rivers and other surface waters.</abstract>
    <parentTitle language="eng">Journal of agricultural and food chemistry</parentTitle>
    <identifier type="old">23460</identifier>
    <identifier type="doi">10.1021/jf903937d</identifier>
    <identifier type="issn">0021-8561</identifier>
    <identifier type="issn">1520-5118</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">12.04.2010</enrichment>
    <author>D.L.D. Lima</author>
    <author>Rudolf Schneider</author>
    <author>H.W. Scherer</author>
    <author>A.C. Duarte</author>
    <author>E.B.H. Santos</author>
    <author>V.I. Esteves</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Capillary electrophoresis</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Atrazine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Sorption</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Soil</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Compost</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Sewage sludge</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Farmyard manure</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>23196</id>
    <completedYear/>
    <publishedYear>2010</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>293</pageFirst>
    <pageLast>300</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>80</volume>
    <type>article</type>
    <publisherName>Elsevier Science</publisherName>
    <publisherPlace>Kidlington, Oxford</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Effect of long term organic amendments on adsorption-desorption of thiram onto a luvisol soil derived from loess</title>
    <parentTitle language="eng">Chemosphere</parentTitle>
    <identifier type="old">25767</identifier>
    <identifier type="doi">10.1016/j.chemosphere.2010.04.003</identifier>
    <identifier type="issn">0045-6535</identifier>
    <identifier type="issn">0366-7111</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">08.02.2011</enrichment>
    <author>O.M.S. Filipe</author>
    <author>M.M. Vidal</author>
    <author>H.W. Scherer</author>
    <author>Rudolf Schneider</author>
    <author>A. Duarte</author>
    <author>V.I. Esteves</author>
    <author>E.B.H. Santos</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Thiram</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Adsorption-desorption</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Soil</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Organic amendments</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
  <doc>
    <id>24305</id>
    <completedYear/>
    <publishedYear>2011</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1494</pageFirst>
    <pageLast>1499</pageLast>
    <pageNumber/>
    <edition/>
    <issue>3</issue>
    <volume>85</volume>
    <type>article</type>
    <publisherName>Elsevier B.V.</publisherName>
    <publisherPlace>Amsterdam</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Development of an ELISA procedure to study sorption of atrazine onto a sewage sludge-amended luvisol soil</title>
    <abstract language="eng">Pesticides may contaminate ground and surface waters and one of the major factors governing this property is soil sorption. Sorption can be assessed by batch equilibrium technique which produces lots of extracts with high dissolved organic carbon concentration in which the pesticide concentration has to be determined. We developed an ELISA procedure to analyse atrazine based on polyclonal antibodies (C193) for which tracer structure and dilutions of immunochemical reagents were adapted to fit the purpose. After a 1000-fold dilution (or after an SPE clean-up procedure) extracts of a sewage-sludge amended luvisol (used as an example application of the methodology developed) could be reliably analysed. The Freundlich model is able to describe adsorption for this system (r² = 0.977) delivering a distribution coefficient KF of 1.6 ± 0.2 (mg kg-1) (mg L-1)-N and an isotherm nonlinearity factor N of 0.70 ± 0.09.</abstract>
    <parentTitle language="eng">Talanta</parentTitle>
    <identifier type="old">26943</identifier>
    <identifier type="doi">10.1016/j.talanta.2011.06.024</identifier>
    <identifier type="issn">0039-9140</identifier>
    <enrichment key="date_peer_review">15.09.2011</enrichment>
    <author>D.L.D. Lima</author>
    <author>C.P. Silva</author>
    <author>Rudolf Schneider</author>
    <author>V.I. Esteves</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>ELISA</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Atrazine</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Sorption</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Datei im Netzwerk der BAM verfügbar ("Closed Access")</collection>
  </doc>
  <doc>
    <id>24306</id>
    <completedYear/>
    <publishedYear>2011</publishedYear>
    <thesisYearAccepted/>
    <language>eng</language>
    <pageFirst>1708</pageFirst>
    <pageLast>1715</pageLast>
    <pageNumber/>
    <edition/>
    <issue>11</issue>
    <volume>84</volume>
    <type>article</type>
    <publisherName>Elsevier Science</publisherName>
    <publisherPlace>Kidlington, Oxford</publisherPlace>
    <creatingCorporation/>
    <contributingCorporation/>
    <belongsToBibliography>1</belongsToBibliography>
    <completedDate>--</completedDate>
    <publishedDate>--</publishedDate>
    <thesisDateAccepted>--</thesisDateAccepted>
    <title language="eng">Application of an ELISA to the quantification of carbamazepine in ground, surface and wastewaters and validation with LC-MS/MS</title>
    <abstract language="eng">Carbamazepine is a psychiatric pharmaceutical widely detected in aquatic environments. Due to its generalized occurrence and environmental persistence it might be considered as an anthropogenic pollution indicator. In this research, a previously developed enzyme-linked immunosorbent assay (ELISA), based on a commercial monoclonal antibody, was applied to the quantification of carbamazepine in ground, surface and wastewaters and results were validated by liquid chromatographytandem mass spectrometry (LCMS/MS).

The performance of the applied ELISA methodology was tested in the presence of high concentrations of sodium chloride and dissolved organic matter. The method was not significantly affected by matrix effects, being adequate for the quantification of carbamazepine in environmental samples, even without sample pre-treatment. This method allows the quantification of carbamazepine in the range of 0.0310 µg L-1, with a relative error lower than 30%. Due to a pH dependent cross-reactivity with cetirizine, an antihistaminic drug, the assay also enabled the quantification of cetirizine in the samples.

The application of the developed method to the quantification of carbamazepine was performed by using environmental samples with very different matrices, collected in the geographical area of Ria de Aveiro, an estuarine system located in the North of Portugal. Carbamazepine was detected in all analyzed wastewater samples and in one surface water with concentrations between 0.1 and 0.7 µg L-1. Validation with LCMS/MS revealed that results obtained by ELISA are 228% overestimated, which was considered highly satisfactory due to the absence of sample pre-treatments.</abstract>
    <parentTitle language="eng">Chemosphere</parentTitle>
    <identifier type="old">26944</identifier>
    <identifier type="doi">10.1016/j.chemosphere.2011.04.072</identifier>
    <identifier type="issn">0045-6535</identifier>
    <identifier type="issn">0366-7111</identifier>
    <enrichment key="bibliotheksstandort">Sonderstandort: Publica-Schrank</enrichment>
    <enrichment key="date_peer_review">15.09.2011</enrichment>
    <author>V. Calisto</author>
    <author>Arnold Bahlmann</author>
    <author>Rudolf Schneider</author>
    <author>V.I. Esteves</author>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Immunoassay</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Pharmaceuticals</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Environment</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Wastewater treatment plants</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Matrix effects</value>
    </subject>
    <subject>
      <language>eng</language>
      <type>uncontrolled</type>
      <value>Cetirizine</value>
    </subject>
    <collection role="literaturgattung" number="">Verlagsliteratur</collection>
    <collection role="fulltextaccess" number="">Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")</collection>
  </doc>
</export-example>
