26187
2012
eng
1278
1286
11
89
article
Elsevier Science
Kidlington, Oxford
1
--
--
--
Immunoassays as high-throughput tools: monitoring spatial and temporal variations of carbamazepine, caffeine and cetirizine in surface and wastewaters
Carbamazepine (CBZ), caffeine and cetirizine were monitored by enzyme-linked immunosorbent assays (ELISAs) in surface and wastewaters from Berlin, Germany. This fast and cost-efficient method enabled to assess the spatial and temporal variation of these anthropogenic markers in a high-throughput screening. CBZ and cetirizine were detected by the same antibody, which selectively discriminates between both compounds depending on the pH value used in the incubation step. To our best knowledge, this is the first dual-analyte immunoassay working with a single antibody.
The frequent sampling with 487 samples being processed allowed for the repeated detection of unusually high concentrations of CBZ and caffeine. ELISA results correlate well with the ones obtained by liquid chromatography tandem mass spectrometry (LC-MS/MS). Caffeine concentrations found in surface waters were elevated by combined sewer overflows after stormwater events. During the hay fever season, the concentrations of the antihistamine drug cetirizine increased in both surface and wastewaters.
Caffeine was almost completely removed during wastewater treatment, while CBZ and cetirizine were found to be more persistent. The maximum concentrations of caffeine, CBZ and cetirizine found in influent wastewater by LCMS/MS were 470, 5.0 and 0.49 µg L-1, while in effluent wastewater the concentrations were 0.22, 4.5 and 0.51 µg L-1, respectively. For surface waters, concentrations up to 3.3, 4.5 and 0.72 µg L-1 were found, respectively.
Chemosphere
28934
10.1016/j.chemosphere.2012.05.020
0045-6535
0366-7111
31.07.2012
Arnold Bahlmann
José Joao Carvalho
Michael G. Weller
Ulrich Panne
Rudolf Schneider
eng
uncontrolled
ELISA
eng
uncontrolled
Carbamazepine
eng
uncontrolled
Caffeine
eng
uncontrolled
Cetirizine
eng
uncontrolled
Environmental monitoring
eng
uncontrolled
Anthropogenic markers
Verlagsliteratur
Datei im Netzwerk der BAM verfügbar ("Closed Access")
27518
2012
deu
278
281
4
article
GIT-Verlag
Darmstadt
0
--
--
--
ELISA goes Berlin - Bestimmung von Carbamazepin, Cetrizin und Coffein in Berliner Gewässern
GIT : Labor-Fachzeitschrift
30338
0016-3538
Sonderstandort: Publica-Schrank
J.J.D. Carvalho
Arnold Bahlmann
Rudolf Schneider
deu
uncontrolled
ELISA
deu
uncontrolled
Koffein
deu
uncontrolled
Immunoassay
deu
uncontrolled
Oberflächenwasser
deu
uncontrolled
Wasseranalytik
Verlagsliteratur
Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
16655
2008
deu
poster
0
--
--
--
Neue Konzepte für die Kalibrierung und Auswertung von Immunoassays
GDCh-Doktorandentagung
18677
GDCh-Doktorandentagung
Attendorn, Germany
2008-02-10
2008-02-12
Arnold Bahlmann
Weder Datei noch physisches Exemplar vorhanden ("No Access")
Präsentation
23546
2011
eng
1357
1364
7
136
article
Royal Society of Chemistry
Cambridge
1
--
--
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Cetirizine as pH-dependent cross-reactant in a carbamazepine-specific immunoassay
High performance liquid chromatography (HPLC) was hyphenated with a previously reported carbamazepine-specific enzyme-linked immunosorbent assay (ELISA) as a screening approach to water analysis in order to identify possible interferences from transformation products. Treated wastewater was analysed and three substances were recognized by the antibody besides carbamazepine: the metabolites 10,11-dihydro-10,11-epoxycarbamazepine and 2-hydroxycarbamazepine plus the structurally not obviously related antihistamine cetirizine. The molar cross-reactivity against cetirizine was found to be pH-dependent and assessed to be 400% at pH 4.5 and 22% at pH 10.5. Performing the ELISA at pH 10.5 greatly improved the accuracy when carbamazepine was determined in surface and wastewater samples.
Analyst
26126
10.1039/c0an00928h
0003-2654
1364-5528
Sonderstandort: Publica-Schrank
18.04.2011
Arnold Bahlmann
Jana Falkenhagen
Michael G. Weller
Ulrich Panne
Rudolf Schneider
eng
uncontrolled
Immunoassay
eng
uncontrolled
ELISA
eng
uncontrolled
Cetirizin
eng
uncontrolled
Carbamazepin
eng
uncontrolled
pH-Abhängigkeit
eng
uncontrolled
Kreuzreaktivität
Verlagsliteratur
Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
20927
2009
eng
1809
1820
6
395
article
Springer
Berlin
1
--
--
--
Monitoring carbamazepine in surface and wastewaters by an immunoassay based on a monoclonal antibody
The pharmaceutical compound carbamazepine (CBZ) is an emerging pollutant in the aquatic environment and may potentially be used as a wastewater marker. In this work, an enzyme-linked immunosorbent assay (ELISA) for the detection of carbamazepine in surface and sewage waters has been developed. The heterogeneous immunoassay is based on a commercially available monoclonal antibody and a novel enzyme conjugate (tracer) that links the hapten via a hydrophilic peptide (triglycine) spacer to horseradish peroxidase. The assay achieves a limit of detection of 24 ng/L and a quantitation range of 0.05-50 µg/L. The analytical performance and figure of merits were compared to liquid chromatography-tandem mass spectrometry after solid-phase extraction. For nine Berlin surface water samples and one wastewater sample, a close correlation of results was observed. A constant overestimation relative to the CBZ concentration of approximately 30% by ELISA is probably caused by the presence of 10,11-epoxy-CBZ and 2-hydroxy-CBZ in the samples. The ELISA displayed cross-reactivities for these compounds of 83% and 14%, respectively. In a first screening of 27 surface water samples, CBZ was detected in every sample with concentrations between 0.05 and 3.2 µg/L. Since no sample cleanup is required, the assay allowed for the determination of carbamazepine with high sensitivity at low costs and with much higher throughput than with conventional methods.
Analytical and bioanalytical chemistry
23262
10.1007/s00216-009-2958-7
1618-2642
1618-2650
Sonderstandort: Publica-Schrank
25.02.2010
Arnold Bahlmann
Michael G. Weller
Ulrich Panne
Rudolf Schneider
eng
uncontrolled
Carbamazepine
eng
uncontrolled
ELISA
eng
uncontrolled
Antibody
eng
uncontrolled
Immunoassay
eng
uncontrolled
Surface water
eng
uncontrolled
LC-MS/MS
eng
uncontrolled
Pharmaceuticals
Verlagsliteratur
Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
21033
2010
deu
poster
0
--
--
--
Occurence of Caffeine and Carbamazepine in Teltowkanal and Landwarekanal, Berlin
Internationales Symposium "20 Years of Research in the Field of Endocrine Disruptors and Pharmaceutical Compounds - Challenge and Solution for the Water"
23381
Internationales Symposium "20 Years of Research in the Field of Endocrine Disruptors and Pharmaceutical Compounds - Challenge and Solution for the Water"
Berlin, Germany
2010-02-10
Arnold Bahlmann
José Joao Carvalho
Weder Datei noch physisches Exemplar vorhanden ("No Access")
Präsentation
22069
2010
deu
poster
0
--
--
--
Seasonal variations of carbamazepine and cetrizine in Teltowkanal, Berlin, monitored by a dualanalyte immunoassay
3rd EuCheMS Chemistry Congress
24564
3rd EuCheMS Chemistry Congress
Nuremberg, Germany
2010-08-29
2010-09-02
Arnold Bahlmann
Weder Datei noch physisches Exemplar vorhanden ("No Access")
Präsentation
32070
2014
deu
lecture
0
--
--
--
Carbamazepin und seine Metaboliten in Abwasser, Oberflächenwasser und Trinkwasser
LC-MS in der Umweltanalytik 2014
35130
LC-MS in der Umweltanalytik 2014
Leipzig
2014-06-16
2014-06-17
0
Arnold Bahlmann
Weder Datei noch physisches Exemplar vorhanden ("No Access")
Präsentation
30615
2014
eng
104
114
57
article
Elsevier
Amsterdam
International Association on Water Pollution Research
1
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--
--
Carbamazepine and its metabolites in wastewater: Analytical pitfalls and occurrence in Germany and Portugal
The occurrence of carbamazepine (CBZ) and its metabolites in German and Portuguese wastewater was investigated. A total of 46 samples from influent and effluent wastewater were analyzed by liquid-chromatography (LC) tandem mass spectrometry. The five metabolites 10,11-dihydro-10,11-dihydroxy-CBZ (DiOH-CBZ), 10,11-dihydro-10-hydroxy-CBZ (10-OH-CBZ), 10,11-epoxy-10,11-dihydro-CBZ, 2-hydroxy-CBZ and 3-hydroxy-CBZ were very persistent with little to no removal during wastewater treatment. The highest concentrations were found for CBZ, DiOH-CBZ, and 10-OH-CBZ, with up to 5.0, 4.8 and 1.1 µg/L, respectively. Furthermore, the related pharmaceutical oxcarbazepine and the metabolites 9-hydroxymethyl-10-carbamoylacridan, 1-hydroxy-CBZ (1-OH-CBZ) and 4-hydroxy-CBZ (4-OH-CBZ) were detected. Explicit care was taken to achieve a good chromatographic separation of the numerous isomers that were difficult to distinguish by mass spectrometry alone. A phenylether stationary phase provided the best separation. In combination with high resolution mass spectrometry and hydrogen-deuterium exchange, this LC column enabled us to identify 1-OH-CBZ and 4-OH-CBZ in wastewater for the first time.
Water research
33591
10.1016/j.watres.2014.03.022
0043-1354
Sonderstandort: Publica-Schrank
28.04.2014
Arnold Bahlmann
W. Brack
Rudolf Schneider
M. Krauss
eng
uncontrolled
Carbamazepine
eng
uncontrolled
Hydroxycarbamazepine
eng
uncontrolled
Metabolite
eng
uncontrolled
Wastewater
eng
uncontrolled
Pharmaceutical
eng
uncontrolled
LCMS/MS
Verlagsliteratur
Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")
21548
2010
deu
lecture
0
--
--
--
Bestimmung des anthropogenen Markers Carbamazepin im Gewässerkreislauf mit Hilfe eines neuentwickelten Immunoassays (ELISA)
Jahrestagung der Wasserchemischen Gesellschaft
23980
Jahrestagung der Wasserchemischen Gesellschaft
Bayreuth, Germany
2010-05-12
0
Arnold Bahlmann
Weder Datei noch physisches Exemplar vorhanden ("No Access")
Präsentation
19129
2009
deu
poster
0
--
--
--
Immunoassay für die Bestimmung von Carbamazepin in Gewässern
ANAKON 2009
21326
ANAKON 2009
Berlin, Germany
2009-03-17
2009-03-20
Arnold Bahlmann
Weder Datei noch physisches Exemplar vorhanden ("No Access")
Präsentation
24306
2011
eng
1708
1715
11
84
article
Elsevier Science
Kidlington, Oxford
1
--
--
--
Application of an ELISA to the quantification of carbamazepine in ground, surface and wastewaters and validation with LC-MS/MS
Carbamazepine is a psychiatric pharmaceutical widely detected in aquatic environments. Due to its generalized occurrence and environmental persistence it might be considered as an anthropogenic pollution indicator. In this research, a previously developed enzyme-linked immunosorbent assay (ELISA), based on a commercial monoclonal antibody, was applied to the quantification of carbamazepine in ground, surface and wastewaters and results were validated by liquid chromatographytandem mass spectrometry (LCMS/MS).
The performance of the applied ELISA methodology was tested in the presence of high concentrations of sodium chloride and dissolved organic matter. The method was not significantly affected by matrix effects, being adequate for the quantification of carbamazepine in environmental samples, even without sample pre-treatment. This method allows the quantification of carbamazepine in the range of 0.0310 µg L-1, with a relative error lower than 30%. Due to a pH dependent cross-reactivity with cetirizine, an antihistaminic drug, the assay also enabled the quantification of cetirizine in the samples.
The application of the developed method to the quantification of carbamazepine was performed by using environmental samples with very different matrices, collected in the geographical area of Ria de Aveiro, an estuarine system located in the North of Portugal. Carbamazepine was detected in all analyzed wastewater samples and in one surface water with concentrations between 0.1 and 0.7 µg L-1. Validation with LCMS/MS revealed that results obtained by ELISA are 228% overestimated, which was considered highly satisfactory due to the absence of sample pre-treatments.
Chemosphere
26944
10.1016/j.chemosphere.2011.04.072
0045-6535
0366-7111
Sonderstandort: Publica-Schrank
15.09.2011
V. Calisto
Arnold Bahlmann
Rudolf Schneider
V.I. Esteves
eng
uncontrolled
Immunoassay
eng
uncontrolled
Pharmaceuticals
eng
uncontrolled
Environment
eng
uncontrolled
Wastewater treatment plants
eng
uncontrolled
Matrix effects
eng
uncontrolled
Cetirizine
Verlagsliteratur
Physisches Exemplar in der Bibliothek der BAM vorhanden ("Hardcopy Access")