Dokument-ID Dokumenttyp Autoren/innen Persönliche Herausgeber/innen Haupttitel Abstract Auflage Verlagsort Verlag Herausgeber (Institution) Erscheinungsjahr Titel des übergeordneten Werkes Jahrgang/Band ISBN Veranstaltung Veranstaltungsort Beginndatum der Veranstaltung Enddatum der Veranstaltung Ausgabe/Heft Erste Seite Letzte Seite URN DOI Lizenz Datum der Freischaltung OPUS4-39038 Zeitschriftenartikel Waiblinger, H. U.; Brockmeyer, J.; Bruenen-Nieweler, C.; Busch, U.; Haase, I.; Hahn, A.; Haarmann, M.; Hauser, W.; Huber, I.; Jany, K. D.; Kirmse, N.; Lindeke, S.; Neumann, K.; Naumann, H.; Paschke, A.; Pietsch, K.; Pöpping, B.; Reiting, R.; Schroeder, U.; Schwägele, F.; Weller, Michael G.; Zagon, J. Methoden zur Differenzierung von Tierarten in Lebensmitteln - Status quo Nicht zuletzt bedingt durch den Pferdefleischskandal im Jahr 2013 wurden in Deutschland die Aktivitäten bei der Methodenstandardisierung im Bereich der Tierarten-Differenzierung in den vergangenen Jahren intensiviert. Wenn auch positive Befunde bei dem Nachweis von Bestandteilen aus Pferd in Lebensmitteln wie Lasagne derzeit so gut wie nicht mehr anzutreffen sind, so hat die Tierartendifferenzierung insgesamt beim Nachweis von Verfälschungen in Lebensmitteln einen hohen Stellenwert. Diese Arbeit fasst daher den aktuellen Stand der Analytik in Deutschland mit Schwerpunkt bei der Standardisierung zusammen. Sie wurde erstellt durch die Arbeitsgruppe „Biochemische und molekularbiologische Analytik" der Lebensmittelchemischen Gesellschaft mit Unterstützung von Experten der Arbeitsgruppe „Molekularbiologische Methoden zur Pflanzen- und Tierartendifferenzierung" (§ 64 LFGB) sowie der ALTS-Arbeitsgruppe „Immunologie und Molekularbiologie" (jeweils D). Frankfurt Deutscher Fachverlag GmbH 2017 Fleischwirtschaft 1 97 102 2017-01-27 OPUS4-43485 Zeitschriftenartikel Waiblinger, H. U.; Bartsch, D.; Brockmeyer, J.; Bruenen-Nieweler, C.; Busch, U.; Haase, I.; Hahn, A.; Haarmann, M.; Hauser, W.; Huber, I.; Jany, K. D.; Kirmse, N.; Lindeke, S.; Neumann, K.; Naumann, H.; Paschke, A.; Pietsch, K.; Pöpping, B.; Reiting, R.; Schroeder, U.; Schwägele, F.; Weller, Michael G.; Zagon, J. Methods of differentiating animal species in food - Status quo In the field of animal species differentiation, work on standardizing methods has been intensified in Germany in recent years, not least due to the horsemeat scandal in 2013. Even though there are now hardly ever any positive findings anymore in examinations to detect horse adulterations in foods such as lasagne, animal species differentiation altogether ranks high in detecting adulteration of foods. This article, therefore, summarises the current status of analytical techniques used in Germany with standardization at German level. It has been established by the working group "Biochemical and Molecular Biological Analytics" of the Lebensmittelchemische Gesellschaft (Food Chemistry Society within the German Chemical Society) with support of experts in the working group "Molecular biology techniques for differentiating plant and animal species" (§64 of the German Food and Feed Code - LFGB) and the "Immunology and molecular biology" task force of the food hygiene and food of animal origin working group (ALTS), both from Germany. Frankfurt am Main Deutscher Fachverlag GmbH 2017 Fleischwirtschaft China 3 29 36 2017-12-19 OPUS4-39264 Zeitschriftenartikel Waiblinger, H. U.; Bartsch, D.; Brockmeyer, J.; Bruenen-Nieweler, C.; Busch, U.; Haase, I.; Hahn, A.; Haarmann, M.; Hauser, W.; Huber, I.; Jany, K. D.; Kirmse, N.; Lindeke, S.; Neumann, K.; Naumann, H.; Paschke, A.; Pietsch, K.; Pöpping, B.; Reiting, R.; Schroeder, U.; Schwägele, F.; Weller, Michael G.; Zagon, J. Methods of differentiating animal species in foods - Status quo Work on standardising methods in the field of animal species differentiation has been intensified in Germany in recent years, not least due to the horsemeat scandal in 2013. Even though there are now hardly ever any positive findings any more in examinations to detect horse adulterations in foods such as lasagne, animal species differentiation altogether ranks high in detecting adulteration of foods. This article therefore summarises the current status of analytical techniques used in Germany with standardisation at German level. It has been established by the working group "Biochemical and Molecular Biological Analytics" of the Lebensmittelchemische Gesellschaft (Food Chemistry Society within the German Chemical Society) with support of experts in the working group "Molecular biology techniques for differentiating plant and animal species" (§ 64 of the German Food and Feed Code - LFGB) and the "Immunology and molecular biology" task force of the food hygiene and Food of animal origin working group (ALTS), both from Germany. Frankfurt Deutscher Fachverlag GmbH 2017 Fleischwirtschaft international 97 1 50 55 2017-03-01 OPUS4-2283 Beitrag zu einem Sammelband Hasche, K.; Ulm, G.; Herrmann, K.; Krumrey, M.; Ade, G.; Sümpel, J.; Busch, I.; Thomsen-Schmidt, P.; Schädlich, S.; Schindler, A.; Frank, W.; Procop, Mathias; Beck, Uwe About the calibration of thickness standards on the nanometer scale Eindhoven European Society for Precision Engineering and Nanotechnology 2002 Proceedings of the 3rd international EUSPEN conference 90-386-2883-8 3rd International EUSPEN conference ; 3rd International conference. European Society for Precision Engineering and Nanotechnology ; 4th General meeting. European Society for Precision Engineering and Nanotechnology Eindhoven, The Netherlands 2002-05-26 2002-05-30 1 5 2016-02-19 OPUS4-2284 Beitrag zu einem Tagungsband Hasche, K.; Herrmann, K.; Thomsen-Schmidt, P.; Krumrey, M.; Ulm, G.; Ade, G.; Pohlenz, F.; Stümpel, J.; Busch, I.; Schädlich, S.; Frank, W.; Hirsch, D.; Schindler, A.; Procop, Mathias; Beck, Uwe Ein Beitrag zu kalibrierten Nanometerschichten für unterschiedliche Anwendungsbereiche Nanotechnologien werden gegenwärtig als leistungsfähige Oberflächentechniken in erheblichem Umfange industriell genutzt. Dabei spielen dünne Schichten und deren Kenndaten eine wesentliche Rolle, z.B. als Röntgenspiegel oder optische und magnetooptische Datenträger. Die Schichtdicke zählt hierbei zu den technologisch wichtigen Parametern. Abgeleitet von Anwenderinteressen, werden für die Röntgenreflektometrie (XRR bzw. GIXR), Elektronenstrahl-Mikroanalyse (EPMA) und Röntgenfluoreszenzanalyse (XRF) einerseits und die Ellipsometrie andererseits zugeschnittene Schichtdickenmaßverkörperungen (SDM) entwickelt, gefertigt und untersucht. Das Ziel ist die Bereitstellung praxistauglicher, kalibrierter SDM, im weiteren Schichtdickennormale (SDN) genannt. SDN sind Maßverkörperungen, für die der Schichtdickenwert mit einer definierten Messunsicherheit bekannt und auf ein metrologisch anerkanntes Längennormal zurückgeführt ist. Berichtet wird über die Herstellung und messtechnische Charakterisierung der beiden spezifischen Varianten von Maßverkörperungen. Ilmenau Techn. Univ. Gesellschaft für Arbeitswissenschaft 2002 47. Internationales Wissenschaftliches Kolloquium 47 47. Internationales Wissenschaftliches Kolloquium. Technische Universität Ilmenau ; Herbstkonferenz der Gesellschaft für Arbeitswissenschaft ; 6. Workshop Multimedia für Bildung und Wirtschaft Ilmenau, Deutschland 2002-09-23 2002-09-26 568 569 2016-02-19 OPUS4-2285 Beitrag zu einem Tagungsband Koenders, L.; Hasche, K.; Ulm, G.; Krumrey, M.; Ade, G.; Stümpel, J.; Busch, I.; Thomsen-Schmidt, P.; Schädlich, S.; Schindler, A.; Frank, W.; Beck, Uwe; Procop, Mathias Über die metrologische Charakterisierung von Schichtdicken Standards im Nanometerbereich 2002 12th Applied Surface Analysis Workshop 12th Applied Surface Analysis Workshop ; AOFA 12 --- 2002-09-15 2002-09-19 2016-02-19 OPUS4-25428 Forschungsbericht Göbel, E. O.; Hasche, K.; Ulm, G.; Krumrey, M.; Ade, G.; Stümpel, J.; Herrmann, K.; Koenders, L.; Thomsen-Schmidt, P.; Pohlenz, F.; Xu, M.; Dai, G.; Hoffmann, K.-P.; Busch, I.; Frank, W.; Procop, M.; Beck, Uwe Kalibrierte Schichtdicken-Maßverkörperungen für Nanometerschichten, Teilvorhaben - Kalibrierung von Schichtdicken-Maßverkörperungen im Nanometerbereich - Abschlussbericht des BMBF Verbundvorhabens 13N7704 - 13N77041 Dresden Physikalisch-Technische Bundesanstalt 2003 1 131 2016-02-19 OPUS4-17195 Zeitschriftenartikel Thomsen-Schmidt, P.; Hasche, K.; Ulm, G.; Herrmann, K.; Krumrey, M.; Ade, G.; Stümpel, J.; Busch, I.; Schädlich, S.; Schindler, A.; Frank, W.; Hirsch, D.; Procop, Mathias; Beck, Uwe Realisation and metrological characterisation of thickness standards below 100 nm High-accuracy film thickness measurements in the range below 100 nm can be made by various complex methods like spectral ellipsometry (SE), scanning force microscopy (SFM), grazing incidence X-ray reflectometry (GIXR), or X-ray fluorescence analysis (XRF). The measurement results achieved with these methods are based on different interactions between the film and the probe. A key question in nanotechnology is how to achieve consistent results on a level of uncertainty below one nanometre with different techniques. Two different types of thickness standards are realised. Metal film standards for X-ray techniques in the thickness range 10 to 50 nm are calibrated by GIXR with monochromatised synchrotron radiation of 8048 eV. The results obtained at four different facilities show excellent agreement. SiO2 on Si standards for SE and SFM in the thickness range 6 to 1000 nm are calibrated by GIXR with monochromatised synchrotron radiation of 1841 eV and with a metrological SFM. Consistent results within the combined uncertainties are obtained with the two methods. Surfaces and interfaces of both types of standards are additionally investigated by transmission electron microscopy (TEM). Berlin Springer 2004 Applied physics A 78 5 645 649 10.1007/s00339-003-2273-7 2016-02-19 OPUS4-26069 Zeitschriftenartikel Busch, C.; Schröter, T.; Grabolle, Markus; Wenzel, M.; Kempe, H.; Kaiser, W.A.; Resch-Genger, Ute; Hilger, I. An in vivo spectral multiplexing approach for the cooperative imaging of different disease-related biomarkers with near-infrared fluorescent Förster resonance energy transfer probes In recent years, much progress has been made in analyzing the molecular origin of many diseases in vivo. For most applications, attention has been devoted to the detection of single molecules only. In this study, we present a proof of concept for the straightforward monitoring of interactions between different molecules via Förster resonance energy transfer (FRET) in an in vivo spectral multiplexing approach using conventional small organic dyes covalently attached to antibodies. Methods: We coupled the fluorophores DY-682 (donor; absorption [abs]/emission [em], 674/712 nm), DY-505 (control donor; abs/em, 498/529 nm), and DY-782 (acceptor; abs/em, 752/795 nm) to the model antibody IgG. The occurrence of FRET between these fluorophores was assessed in vitro for conjugate mixtures adsorbed onto membranes, after accumulation into the phagocytic compartment of macrophages (J774 cells), and in vivo in a mouse edema model using a whole-body animal imaging system with multispectral analysis features. Results: When the free acceptor DY-782 was combined with the DY-682 donor, FRET occurred as a consequence of small dye-to-dye distances, unlike the case for mixtures of the dyes DY-782 and DY-505. Our proof of concept was also transferred to living cells after internalization of the DY-682-IgG–DY-782-IgG pair into macrophages and finally to animals, where intermolecular FRET was observed after systemic probe application in vivo in edema-bearing mice. Conclusion: Our simple cooperative-imaging approach enables the noninvasive detection of the presence of two or principally even more neighboring disease-related biomarkers. This finding is of high relevance for the in vivo identification of complex biologic processes requiring strong spatial interrelations of target molecules in key pathologic activation processes such as inflammation, cancer, and neurodegenerative diseases. Reston, Va. SNM 2012 Journal of nuclear medicine 53 4 638 646 10.2967/jnumed.111.094391 2016-02-19 OPUS4-54175 Zeitschriftenartikel Kim, K.J.; Kim, C.S.; Ruh, S. W.; Unger, Wolfgang; Radnik, Jörg; Mata-Salazar, J.; Juarez-Garcia, J.M.; Cortazar-Martinez, O.; Herrera-Gomez, A.; Hansen, P.E.; Madesen, J.S.; Senna, C.A.; Archanjo, B.S.; Damasceno, J.C.; Achete, C.A.; Wang, H.; Wang, M.; Windover, D.; Steel, E.; Kurokawa, A.; Fujimoto, T.; Azuma, Y.; Terauchi, S.; Zhang, L.; Jordaan, W.A.; Spencer, S.J.; Shard, A.G.; Koenders, L.; Krumrey, M.; Busch, I.; Jeynes, C. Thickness measurement of nm HfO2 films A pilot study for the thickness measurement of HfO2 films was performed by the Surface Analysis Working Group (SAWG) of the Consultative Committee for Amount of Substance (CCQM). The aim of this pilot study was to ensure the equivalency in the measurement capability of national metrology institutes for the thickness measurement of HfO2 films. In this pilot study, the thicknesses of six HfO2 films with nominal thickness from 1 nm to 4 nm were measured by X-ray Photoelectron Spectroscopy (XPS), X-ray Reflectometry(XRR), X-ray Fluorescence Analysis (XRF), Transmission Electron Spectroscopy (TEM), Spectroscopic Ellipsometry (SE) and Rutherford Backscattering Spectrometry (RBS). The reference thicknesses were determined by mutual calibration of a zero-offset method (Medium Energy Ion Scattering Spectroscopy (MEIS) of KRISS) and a method traceable to the length unit (the average thicknesses of three XRR data except the thinnest film). These reference thicknesses are traceable to the length unit because they are based on the traceability of XRR. For the thickness measurement by XPS, the effective attenuation length of Hf 4f electrons was determined. In the cases of XRR and TEM, the offset values were determined from a linear fitting between the reference thicknesses and the individual data by XRR and TEM. The amount of substance of HfO2, expressed as thickness of HfO2 films (in both linear and areal density units), was found to be a good subject for a CCQM key comparison. To reach the main text of this paper, click on Final Report. The final report has been peer-reviewed and approved for publication by the CCQM. Bristol IOP Publishing Lt. 2021 Metrologia 58 1a 08016 10.1088/0026-1394/58/1A/08016 2022-01-03 OPUS4-53073 Zeitschriftenartikel Tielemann, Christopher; Busch, R.; Reinsch, Stefan; Patzig, C.; Höche, T.; Avramov, I.; Müller, Ralf Oriented surface nucleation in diopside glass Es wird die Texturbildung in kristallisierendem Diopsidglas im Zusammenhang mit der Oberflächenbeschaffenheit der unbehandelten Probe untersucht. Zudem wird der diskutiert, dass es sich bei der Texturbildung in Gläsern höchstwahrscheinlich um ein Nukleationsphänomen handelt welches auf die richtungsabhängige Grenzflächenenergie der kristallisierenden Phase zurückzuführen ist. Elsevier B.V. 2021 Journal of Non-Crystalline Solids 562 10.1016/j.jnoncrysol.2021.120661 2021-08-09 OPUS4-33837 Beitrag zu einem Tagungsband Bütefisch, S.; Weimann, T.; Busch, I.; Danzebrink, H.-U.; Gross, Thomas; Unger, Wolfgang; Wirth, Thomas New reference material for imaging XPS (X-ray photoelectron spectroscopy) instrument characterization Reference materials without variations in topography are essential for the characterization of imaging XPS (X-ray Photoelectron Spectroscopy) Instruments. Therefore a new fabrication process for this kind of zero-topography reference material was developed at PTB and resulted in first prototypes. The fabrication process and first measurement results will be presented in this paper. AMA Service GmbH 2015 Sensor 2015 - 17th International conference on sensors and measurement technology (Proceedings - AMA conferences 2015) 978-3-9813484-8-4 Sensor 2015 - 17th International conference on sensors and measurement technology Nuremberg, Germany 19.05.2015 21.05.2015 Chapter P7, 821 825 10.5162/sensor2015/P7.1 2016-02-20 OPUS4-45156 Posterpräsentation Busch, I.; Unger, Wolfgang Analytical and topographical Reference Material for the Nanoscale Reliable standards are required to support research and development as well as end-user in application. Appropriate standards have to fulfill three requirements: small uncertainty, easy to use and low overall costs of application. For calibration of microscopes at nanoscale and/or element analysis special requirements for standards are given, which are challenging in manufacture. 2018 Workshop on Reference Nanomaterials Berlin, Germany 14.05.2018 2018-06-13 OPUS4-50990 Zeitschriftenartikel Stockmann, Jörg M.; Radnik, Jörg; Bütefisch, S.; Busch, I.; Weimann, T.; Passiu, C.; Rossi, A.; Unger, Wolfgang A new test specimen for the determination of the field of view of small-area X-ray photoelectron spectrometers Small-area/spot photoelectron spectroscopy (SAXPS) is a powerful tool for the investigation of small surface features like microstructures of electronic devices, sensors or other functional surfaces, and so forth. For evaluating the quality of such microstructures, it is often crucial to know whether a small signal in a spectrum is an unwanted contamination of the field of view (FoV), defined by the instrument settings, or it originated from outside. To address this issue, the d80/20 parameter of a line scan across a chemical edge is often used. However, the typical d80/20 parameter does not give information on contributions from the long tails of the X-ray beam intensity distribution or the electron-optical system as defined by apertures. In the VAMAS TWA2 A22 project "Applying planar, patterned, multi-metallic samples to assess the impact of analysis area in surface-chemical analysis," new test specimen was developed and tested. The here presented testing material consists of a silicon wafer substrate with an Au-film and embedded Cr circular and square spots with decreasing dimensions from 200 μm down to 5 μm. The spot sizes are traceable to the length unit due to size measurements with a metrological SEM. For the evaluation of the FoV, we determined the Au4f intensities measured with the center of the FoV aligned with the center of the spot and normalized to the Au4f intensity determined on the Au-film. With this test specimen, it was possible to characterize, as an example, the FoV of a Kratos AXIS Ultra DLD XPS instrument. John Wiley & Sons Ltd 2020 Surface and Interface Analysis 52 ECASIA 2019 Dresden, Germany 15.09.2019 20.09.2019 12 890 894 urn:nbn:de:kobv:b43-509902 10.1002/sia.6831 https://creativecommons.org/licenses/by/4.0/deed.de 2020-07-09 OPUS4-43138 Zeitschriftenartikel Unger, Wolfgang; Senoner, Mathias; Wirth, Thomas; Bütefisch, S.; Busch, I. Lateral resolution delivered by imaging surface-analytical instruments as SIMS, AES and XPS: Application of the BAM-L200 Certified Reference Material and related ISO Standards The certified reference material BAM-L200, a nanoscale stripe pattern for length calibration and specification of lateral resolution, is described. BAM-L200 is prepared from a cross-sectioned epitaxially grown layer stack of AlxGa1-xAs and InxGa1 xAs on a GaAs substrate. The surface of BAM-L200 provides a flat pattern with stripe widths ranging down to 1 nm. Calibration distances, grating periods and stripe widths have been certified by TEM with traceability to the length unit. The combination of gratings, isolated narrow stripes and sharp edges of wide stripes offers a plenty of options for the determination of lateral resolution, sharpness and calibration of length scale at selected settings of imaging surface analytical instruments. The feasibility of the reference material for an analysis of the lateral resolution is demonstrated in detail by evaluation of ToF-SIMS, AES and EDX images. Other applications developed in the community are summarized, too. BAM-L200 fully supports the implementation of the revised International Standard ISO 18516 (in preparation) which is based on knowledge outlined in the Technical Report ISO/TR 19319:2013. The Surface Analysis Society of Japan (SASJ) 2017 Journal of Surface Analysis 24 2 123 128 2017-11-28