Dokument-ID Dokumenttyp Autoren/innen Persönliche Herausgeber/innen Haupttitel Abstract Auflage Verlagsort Verlag Herausgeber (Institution) Erscheinungsjahr Titel des übergeordneten Werkes Jahrgang/Band ISBN Veranstaltung Veranstaltungsort Beginndatum der Veranstaltung Enddatum der Veranstaltung Ausgabe/Heft Erste Seite Letzte Seite URN DOI Lizenz Datum der Freischaltung OPUS4-45607 Zeitschriftenartikel Lauer, Franziska; Diehn, Sabrina; Seifert, Stephan; Kneipp, Janina; Sauerland, V.; Barahona, C.; Weidner, Steffen Multivariate analysis of MALDI imaging mass spectrometry data of mixtures of single pollen grains Mixtures of pollen grains of three different species (Corylus avellana, Alnus cordata, and Pinus sylvestris) were investigated by matrixassisted laser desorption/ionization time-of-flight imaging mass spectrometry (MALDI-TOF imaging MS). The amount of pollen grains was reduced stepwise from > 10 to single pollen grains. For sample pretreatment, we modified a previously applied approach, where any additional extraction steps were omitted. Our results show that characteristic pollen MALDI mass spectra can be obtained from a single pollen grain, which is the prerequisite for a reliable pollen classification in practical applications. MALDI imaging of laterally resolved pollen grains provides additional information by reducing the complexity of the MS spectra of mixtures, where frequently peak discrimination is observed. Combined with multivariate statistical analyses, such as principal component analysis (PCA), our approach offers the chance for a fast and reliable identification of individual pollen grains by mass spectrometry. Springer 2018 Journal of the American Society for Mass Spectrometry 29 11 2237 2247 10.1007/s13361-018-2036-5 2018-07-30 OPUS4-39288 Zeitschriftenartikel Lauer, Franziska; Seifert, Stephan; Kneipp, Janina; Weidner, Steffen Simplifying the Preparation of Pollen Grains for MALDI-TOF MS Classification Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a well-implemented analytical technique for the investigation of complex biological samples. In MS, the sample preparation strategy is decisive for the success of the measurements. Here, sample preparation processes and target materials for the investigation of different pollen grains are compared. A reduced and optimized sample preparation process prior to MALDI-TOF measurement is presented using conductive carbon tape as target. The application of conductive tape yields in enhanced absolute signal intensities and mass spectral pattern information, which leads to a clear separation in subsequent pattern analysis. The results will be used to improve the taxonomic differentiation and identification, and might be useful for the development of a simple routine method to identify pollen based on mass spectrometry. Basel, Switzerland MDPI AG MDPI AG 2017 International Journal of Molecular Sciences 18 3 543 554 urn:nbn:de:kobv:b43-392885 10.3390/ijms18030543 http://creativecommons.org/licenses/by/3.0/de/deed.de 2017-03-07 OPUS4-41733 Zeitschriftenartikel Lauer, Franziska; Seifert, Stephan; Kneipp, Janina; Weidner, Steffen Simplifying the preparation of pollen grains for MALDI-TOF MS classification Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) is a well-implemented analytical technique for the investigation of complex biological samples. In MS, the sample preparation strategy is decisive for the success of the measurements. Here, sample preparation processes and target materials for the investigation of different pollen grains are compared. A reduced and optimized sample preparation process prior to MALDI-TOF measurement is presented using conductive carbon tape as target. The application of conductive tape yields in enhanced absolute signal intensities and mass spectral pattern information, which leads to a clear separation in subsequent pattern analysis. The results will be used to improve the taxonomic differentiation and identification, and might be useful for the development of a simple routine method to identify pollen based on mass spectrometry. MDPI 2017 International Journal of Molecular Sciences 18 3 543-1 543-11 10.3390/ijms18030543 2017-08-29