Dokument-ID Dokumenttyp Autoren/innen Persönliche Herausgeber/innen Haupttitel Abstract Auflage Verlagsort Verlag Herausgeber (Institution) Erscheinungsjahr Titel des übergeordneten Werkes Jahrgang/Band ISBN Veranstaltung Veranstaltungsort Beginndatum der Veranstaltung Enddatum der Veranstaltung Ausgabe/Heft Erste Seite Letzte Seite URN DOI Lizenz Datum der Freischaltung OPUS4-45871 Zeitschriftenartikel Santos de Freitas, M.; Araghi, R. R.; Brandenburg, E.; Leiterer, Jork; Emmerling, Franziska; Folmert, K.; Gerling-Driessen, U. I. M.; Bardiaux, B.; Böttcher, C.; Pagel, K.; Diehl, A.; v. Berlepsch, H.; Oschkinat, H.; Koksch, B. The protofilament architecture of a de novo designed coiled coil-based amyloidogenic peptide Amyloid fibrils are polymers formed by proteins under specific conditions and in many cases they are related to pathogenesis, such as Parkinson's and Alzheimer's diseases. Their hallmark is the presence of a β-sheet structure. High resolution structural data on these systems as well as information gathered from multiple complementary analytical techniques is needed, from both a fundamental and a pharmaceutical perspective. Here, a previously reported de novo designed, pH-switchable coiled coil-based peptide that undergoes structural transitions resulting in fibril formation under physiological conditions has been exhaustively characterized by transmission electron microscopy (TEM), cryo-TEM, atomic force microscopy (AFM), wide-angle X-ray scattering (WAXS) and solid-state NMR (ssNMR). Overall, a unique 2-dimensional carpet-like assembly composed of large coexisiting ribbon-like, tubular and funnel-like structures with a clearly resolved protofilament substructure is observed. Whereas electron microscopy and scattering data point somewhat more to a hairpin model of β-fibrils, ssNMR data obtained from samples with selectively labelled peptides are in agreement with both, hairpin structures and linear arrangements. Elsevier 2018 Journal of Structural Biology 203 3 263 272 urn:nbn:de:kobv:b43-458713 10.1016/j.jsb.2018.05.009 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.de 2018-09-07