Dokument-ID Dokumenttyp Autoren/innen Persönliche Herausgeber/innen Haupttitel Abstract Auflage Verlagsort Verlag Herausgeber (Institution) Erscheinungsjahr Titel des übergeordneten Werkes Jahrgang/Band ISBN Veranstaltung Veranstaltungsort Beginndatum der Veranstaltung Enddatum der Veranstaltung Ausgabe/Heft Erste Seite Letzte Seite URN DOI Lizenz Datum der Freischaltung OPUS4-10824 Zeitschriftenartikel Resch-Genger, Ute; Pfeifer, Dietmar; Monte, Christian; Pilz, Walter; Hoffmann, Angelika; Spieles, Monika; Rurack, Knut; Hollandt, J.; Taubert, D.; Schönenberger, B.; Nording, P. Traceability in Fluorometry: Part II. Spectral Fluorescence Standards The need for the traceable characterization of fluorescence instruments is emphasized from a chemist’s point of view, focusing on spectral fluorescence standards for the determination of the wavelength- and polarization-dependent relative spectral responsivity and relative spectral irradiance of fluorescence measuring systems, respectively. In a first step, major sources of error of fluorescence measurements and instrument calibration are revealed to underline the importance of this issue and to illustrate advantages and disadvantages of physical and chemical transfer standards for generation of spectral correction curves. Secondly, examples for sets of traceable chemical emission and excitation standards are shown that cover a broad spectral region and simple procedures for the determination of corrected emission spectra with acceptable uncertainties are presented. With proper consideration of the respective measurement principle and geometry, these dye-based characterization procedures can be not only applied to spectrofluorometers but also to other types of fluorescence measuring systems and even to Raman spectrometers. New York, NY Plenum Publ. Corp. 2005 Journal of fluorescence 15 3 315 336 10.1007/s10895-005-2629-9 2016-02-19 OPUS4-10825 Zeitschriftenartikel Hollandt, J.; Taubert, D.; Seidel, J.; Resch-Genger, Ute; Gugg-Helminger, A.; Pfeifer, Dietmar; Monte, Christian; Pilz, Walter Traceability in Fluorometry - Part I: Physical Standards The inter-instrument, inter-laboratory, and long-term comparability of fluorescence data requires the correction of the measured emission and excitation spectra for the wavelength- and polarization-dependent spectral irradiance of the excitation channel at the sample position and the spectral responsivity of the emission channel employing procedures that guarantee traceability to the respective primary standards. In this respect the traceability chain of fluorometry is discussed from a radiometrist’s point of view. This involves, in a first step, the realization of the spectral radiance scale, based on the blackbody radiator and electron storage ring, and the spectral responsivity scale, based on the cryogenic radiometer and their control via key comparisons of the national metrology institutes. In a second step, the characterization including state-of-the art uncertainties of the respective source and detector transfer standards such as tungsten strip lamps, integrating sphere radiators, and trap detectors used to disseminate these radiometric quantities to users of spectroscopic techniques is presented. New York, NY Plenum Publ. Corp. 2005 Journal of fluorescence 15 3 301 313 10.1007/s10895-005-2628-x 2016-02-19 OPUS4-11556 Zeitschriftenartikel Resch-Genger, Ute; Hoffmann, Katrin; Monte, Christian; Pfeifer, Dietmar Standards in Fluorescence Spectroscopy Darmstadt GIT Verl. 2005 GIT laboratory journal Europe 9 6 29 31 2016-02-19 OPUS4-14206 Zeitschriftenartikel Pfeifer, Dietmar; Hoffmann, Katrin; Hoffmann, Angelika; Monte, Christian; Resch-Genger, Ute The Calibration Kit Spectral Fluorescence Standards - A Simple and Certified Tool for the Standardization of the Spectral Characteristics of Fluorescence Instruments With the Calibration Kit Spectral Fluorescence Standards BAM-F001–BAM-F005, we developed a simple tool for the characterization of the relative spectral responsivity and the long-term stability of the emission channel of fluorescence instruments under routine measurement conditions thereby providing the basis for an improved comparability of fluorescence measurements and eventually standardization. This first set of traceable fluorescence standards, which links fluorescence measurements to the spectral radiance scale in the spectral range of 300–770 nm and has been optimized for spectrofluorometers, can be employed for different measurement geometries and can be adapted to different fluorescence techniques with proper consideration of the underlying measurement principles. New York, NY Plenum Publ. Corp. 2006 Journal of fluorescence 16 4 581 587 10.1007/s10895-006-0086-8 2016-02-19