Dokument-ID Dokumenttyp Autoren/innen Persönliche Herausgeber/innen Haupttitel Abstract Auflage Verlagsort Verlag Herausgeber (Institution) Erscheinungsjahr Titel des übergeordneten Werkes Jahrgang/Band ISBN Veranstaltung Veranstaltungsort Beginndatum der Veranstaltung Enddatum der Veranstaltung Ausgabe/Heft Erste Seite Letzte Seite URN DOI Lizenz Datum der Freischaltung OPUS4-38698 Zeitschriftenartikel Hahn, Marc Benjamin; Meyer, Susann; Schröter, Maria-Astrid; Seitz, H.; Kunte, Hans-Jörg; Solomun, Tihomir; Sturm, Heinz Direct electron irradiation of DNA in fully aqueous environment. Damage determination in combination with Monte Carlo simulations We report on a study in which plasmid DNA in water was irradiated with 30 keV electrons generated by a scanning electron microscope and passed through a 100 nm thick Si3N4 membrane. The corresponding Monte Carlo simulations suggest that the kinetic energy spectrum of the electrons throughout the water is dominated by low energy electrons (<100 eV). The DNA radiation damage, single-strand breaks (SSB) and double-strand breaks (DSB), was determined by electrophoresis. The median lethal dose of D1/2 = 1.7 ± 0.3 Gy was found to be much smaller compared to partially or fully hydrated DNA irradiated under vacuum conditions. The ratio of DSB to SSB was found to be (1:12) as compared to 1:88) found for hydrated DNA. Our method enables quantitative measurements of radiation damage to biomolecules (DNA, proteins) in solutions under varying conditions (pH, salinity, cosolutes) for an electron energy range which is difficult to probe by standard methods. Royal Society of Chemistry 2017 Physical Chemistry Chemical Physics 19 3 1798 1805 urn:nbn:de:kobv:b43-386981 10.1039/C6CP07707B http://creativecommons.org/licenses/by/3.0/de/deed.de 2017-01-19 OPUS4-51182 Zeitschriftenartikel Wittmar, J.; Meyer, Susann; Sieling, T.; Kunte, Hans-Jörg; Smiatek, Jens; Brand, Izabella What Does Ectoine Do to DNA? A Molecular-Scale Picture of Compatible Solute−Biopolymer Interactions Compatible solutes are accumulated in the cytoplasm of halophilic microorganisms. These molecules enable their survival in a high salinity environment. Ectoine is such a compatible solute. It is a zwitterionic molecule which strongly interacts with surrounding water molecules and changes the dynamics of the local hydration shell. Ectoine interacts with biomolecules such as lipids, proteins and DNA. The molecular interaction between ectoine and biomolecules in particular the interaction between ectoine and DNA is far from being understood. In this paper we describe molecular aspects of the interaction between ectoine and double stranded DNA(dsDNA). Two 20 base pairs long dsDNA fragments were immobilized on a Gold surface via a thiol-tether. The interaction between the dsDNA monolayers with diluted and concentrated ectoine solutions was examined by means of X-ray photoelectron and polarization modulation infrared reflection absorption spectroscopies (PM IRRAS). Experimental results indicate that the ability of ectoine to bind water reduces the strength of hydrogen bonds formed to the ribose-phosphate backbone in the dsDNA. In diluted (0.1 M) ectoine solution, DNA interacts predominantly with water molecules. The sugar-phosphate backbone is involved in the formation of strong hydrogen bonds to water, which with elapsing time leads to a reorientation of the planes of nucleic acid bases. This reorientation destabilizes the hydrogen bonds strength between the bases and leads to a partial dehybridizaiton of the dsDNA. In concentrated ectoine solution (2.5 M), almost all water molecules interact with ectoine. Under this condition ectoine is able to interact directly with DNA. Density functional theory (DFT) calculations demonstrate that the direct interaction involves the nitrogen atoms in ectoine and phosphate groups in the DNA molecule. The results of the quantum chemical calculations Show that rearrangements in the ribose-phosphate backbone, caused by a direct interaction with ectoine, facilitates contacts between O atom in the phosphate group and H atoms in a nucleic acid base. In the PM IRRA spectra, an increase in the number of the IR absorption modes in the base pair frequency region proves that the hydrogen bonds between bases become weaker. Thus, a sequence of reorientations caused by interaction with ectoine leads to a breakdown of hydrogen bonds between bases in the double helix. ACS Publicatios 2020 The Journal of Physical Chemistry 124 37 7999 8011 10.1021/acs.jpcb.0c05273 2020-09-10 OPUS4-35835 Posterpräsentation Meyer, Susann Structural changes in plasmid DNA verified by gel electrophoresis and AFM: Sample radiation and damage detection Many microorganisms possess circular molecules of plasmid DNA. Their conformation may vary from more compact to relaxed forms. Structural changes have important consequences for the physiological role of plasmids and will influence plasmid transcription, replication and their susceptibility to environmental stresses. Radiation is a common form of stress and can cause structural damage to DNA such as single (SSB) and double strand breaks (DSB). Recently, it has been indicated that secondary low-energy electrons play an important role in biological damage under vacuum condition. Therefore, our studies focus on irradiating the plasmid pUC19 with low-energy electrons for the first time under physiological conditions. 2016 AFM BioMed Conference Porto, Portugal 11.04.2016 15.04.2016 2016-04-28 OPUS4-35800 Zeitschriftenartikel Hahn, Marc Benjamin; Solomun, Tihomir; Wellhausen, Robert; Herrmann, S; Seitz, H; Meyer, Susann; Kunte, Hans-Jörg; Zeman, J.; Uhlig, F; Smiatek, J; Sturm, Heinz Influence of the Compatible Solute Ectoine on the Local Water Structure: Implications for the Binding of the Protein G5P to DNA Microorganisms accumulate molar concentrations of compatible solutes like ectoine to prevent proteins from denaturation. Direct structural or spectroscopic information on the mechanism and about the hydration shell around ectoine are scarce. We combined surface plasmon resonance (SPR), confocal Raman spectroscopy, molecular dynamics simulations, and density functional theory (DFT) calculations to study the local hydration shell around ectoine and its influence on the binding of a gene-S-protein (G5P) to a single-stranded DNA (dT(25)). Due to the very high hygroscopicity of ectoine, it was possible to analyze the highly stable hydration shell by confocal Raman spectroscopy. Corresponding molecular dynamics simulation results revealed a significant change of the water dielectric constant in the presence of a high molar ectoine concentration as compared to pure water. The SPR data showed that the amount of protein bound to DNA decreases in the presence of ectoine, and hence, the protein-DNA dissociation constant increases in a concentration-dependent manner. Concomitantly, the Raman spectra in terms of the amide I region revealed large changes in the protein secondary structure. Our results indicate that ectoine strongly affects the molecular recognition between the protein and the oligonudeotide, which has important consequences for osmotic regulation mechanisms. 2015 The journal of physical chemistry / B 119 49 15212 15220 10.1021/acs.jpcb.5b09506 2016-04-21 OPUS4-33006 Posterpräsentation Meyer, Susann The damaging effect of low energy electrons on biomolecules and cells: Mechanism of action of compatible solutes 2015 Deutsche Physikalische Gesellschaft (DPG) 2015 Deutsche Physikalische Gesellschaft (DPG) 2015 Berlin, Deutschland 2015-03-15 2015-03-20 2016-02-20 OPUS4-40424 Zeitschriftenartikel Hahn, Marc Benjamin; Meyer, Susann; Kunte, Hans-Jörg; Solomun, Tihomir; Sturm, Heinz Measurements and simulations of microscopic damage to DNA in water by 30 keV electrons: A general approach applicable to other radiation sources and biological targets The determination of the microscopic dose-damage relationship for DNA in an aqueous environment is of a fundamental interest for dosimetry and applications in radiation therapy and protection. We combine geant4 particle-scattering simulations in water with calculations concerning the movement of biomolecules to obtain the energy deposit in the biologically relevant nanoscopic volume. We juxtaposition these results to the experimentally determined damage to obtain the dose-damage relationship at a molecular level. This approach is tested for an experimentally challenging system concerning the direct irradiation of plasmid DNA (pUC19) in water with electrons as primary particles. Here a microscopic target model for the plasmid DNA based on the relation of lineal energy and radiation quality is used to calculate the effective target volume. It was found that on average fewer than two ionizations within a 7.5-nm radius around the sugar-phosphate backbone are sufficient to cause a single strand break, with a corresponding median lethal energy deposit being E1/2=6±4 eV. The presented method is applicable for ionizing radiation (e.g., γ rays, x rays, and electrons) and a variety of targets, such as DNA, proteins, or cells. USA American Physical Society American Physical Society 2017 Physical Review E 95 5 052419-1 052419-8 urn:nbn:de:kobv:b43-404244 10.1103/PhysRevE.95.052419 http://creativecommons.org/licenses/by/3.0/de/deed.de 2017-05-31 OPUS4-41313 Zeitschriftenartikel Meyer, Susann; Schröter, Maria-Astrid; Hahn, Marc Benjamin; Solumon, Tihomir; Strum, Heinz; Kunte, Hans-Jörg Ectoine can enhance structural changes in DNA in vitro Strand breaks and conformational changes of DNA have consequences for the physiological role of DNA. The natural protecting molecule ectoine is beneficial to entire bacterial cells and biomolecules such as proteins by mitigating detrimental effects of environmental stresses. It was postulated that ectoine-like molecules bind to negatively charged spheres that mimic DNA surfaces. We investigated the effect of ectoine on DNA and whether ectoine is able to protect DNA from damages caused by ultraviolet radiation (UV-A). In order to determine different isoforms of DNA, agarose gel electrophoresis and atomic force microscopy experiments were carried out with plasmid pUC19 DNA. Our quantitative results revealed that a prolonged incubation of DNA with ectoine leads to an increase in transitions from supercoiled (undamaged) to open circular (single-strand break) conformation at pH 6.6. The effect is pH dependent and no significant changes were observed at physiological pH of 7.5. After UV-A irradiation in ectoine solution, changes in DNA conformation were even more pronounced and this effect was pH dependent. We hypothesize that ectoine is attracted to the negatively charge surface of DNA at lower pH and therefore fails to act as a stabilizing agent for DNA in our in vitro experiments. 2017 Scientific Reports 7 1 Article 7170, 1 10 urn:nbn:de:kobv:b43-413139 10.1038/s41598-017-07441-z http://creativecommons.org/licenses/by/3.0/de/deed.de 2017-08-14 OPUS4-42828 Zeitschriftenartikel Schröter, Maria-Astrid; Meyer, Susann; Hahn, Marc Benjamin; Solomun, Tihomir; Sturm, Heinz; Kunte, Hans-Jörg Ectoine protects DNA from damage by ionizing radiation Ectoine plays an important role in protecting biomolecules and entire cells against environmental stressors such as salinity, freezing, drying and high temperatures. Recent studies revealed that ectoine also provides effective protection for human skin cells from damage caused by UV-A radiation. These protective properties make ectoine a valuable compound and it is applied as an active ingredient in numerous pharmaceutical devices and cosmetics. Interestingly, the underlying mechanism resulting in protecting cells from radiation is not yet fully understood. Here we present a study on ectoine and its protective influence on DNA during electron irradiation. Applying gel electrophoresis and atomic force microscopy, we demonstrate for the first time that ectoine prevents DNA strand breaks caused by ionizing electron radiation. The results presented here point to future applications of ectoine for instance in cancer radiation therapy. Nature 2017 Scientific Reports 7 1 15272, 1 15272, 7 urn:nbn:de:kobv:b43-428287 10.1038/s41598-017-15512-4 http://creativecommons.org/licenses/by/3.0/de/deed.de 2017-11-13 OPUS4-41164 Beitrag zu einem Tagungsband Hahn, Marc Benjamin; Solomun, Tihomir; Meyer, Susann; Kunte, Hans-Jörg; Schröter, Maria-Astrid; Sturm, Heinz Development of a standard procedure for the irradiation of biomolecules In dosimetry the determination of the effectiveness of the damaging processes is standardized and accounted for by the radiation and tissue weighting factor. For the underlying constituents of the tissue, that is the various biomolecules, such a systematic approach doesn't exist. This makes it difficult to compare results obtained under different experimental conditions. In the following work, we will describe a method to obtain comparable values for the radiation-biomolecule interaction, measured under different conditions. This approach can lead to standardization of dosedamage relationship at the molecular level. Such approach is necessary for a better understanding of the relations between the damage of the single constituents of biological tissue and the whole - finally gaining a more complete picture of irradiation damage. International Raditation Protection Association 2017 Proceedings of the 14th IRPA International Congress 14 14th International Congress of the International Raditation Protection Association (IRPA) Cape Town, South Africa 09.05.2016 12.05.2016 1 5 2017-08-03 OPUS4-46327 Zeitschriftenartikel Oprzeska-Zingrebe, E. A.; Meyer, Susann; Roloff, Alexander; Kunte, Hans-Jörg; Smiatek, J. Influence of compatible solute ectoine on distinct DNA structures: thermodynamic insights into molecular binding mechanisms and destabilization effects In nature, the cellular environment of DNA includes not only water and ions, but also other components and co-solutes, which can exert both stabilizing and destabilizing effects on particular oligonucleotide conformations. Among them, ectoine, known as an important osmoprotectant organic co-solute in a broad range of pharmaceutical products, turns out to be of particular relevance. In this article, we study the influence of ectoine on a short single-stranded DNA fragment and on double-stranded helical B-DNA in aqueous solution by means of atomistic molecular dynamics (MD) simulations in combination with molecular theories of solution. Our results demonstrate a conformation-dependent binding behavior of ectoine, which favors the unfolded state of DNA by a combination of electrostatic and dispersion interactions. In conjunction with the Kirkwood-Buff theory, we introduce a simple Framework to compute the influence of ectoine on the DNA melting temperature. Our findings reveal a significant linear decrease of the melting temperature with increasing ectoine concentration, which is found to be in qualitative agreement with results from denaturation experiments. The outcomes of our Computer simulations provide a detailed mechanistic rationale for the surprising destabilizing influence of ectoine on distinct DNA structures. Royal Society of Chemistry 2018 Phys.Chem.Chem.Phys. 20 40 25861 25874 10.1039/c8cp03543a 2018-10-19