Dokument-ID Dokumenttyp Autoren/innen Persönliche Herausgeber/innen Haupttitel Abstract Auflage Verlagsort Verlag Herausgeber (Institution) Erscheinungsjahr Titel des übergeordneten Werkes Jahrgang/Band ISBN Veranstaltung Veranstaltungsort Beginndatum der Veranstaltung Enddatum der Veranstaltung Ausgabe/Heft Erste Seite Letzte Seite URN DOI Lizenz Datum der Freischaltung OPUS4-54662 Zeitschriftenartikel Beyer, S.; Kimani, Martha Wamaitha; Zhang, Y.; Verhassel, A.; Sternbæk, L.; Wang, T.; Persson, J. L.; Härkönen, P.; Johansson, E.; Caraballo, R.; Elofsson, M.; Gawlitza, Kornelia; Rurack, Knut; Ohlsson, L.; El-Schich, Z.; Gjörloff Wingren, A.; Stollenwerk, M. M. Fluorescent Molecularly Imprinted Polymer Layers against Sialic Acid on Silica-coated Polystyrene Cores - Assessment of the Binding Behavior to Cancer Cells Sialic acid (SA) is a monosaccharide usually linked to the terminus of glycan chains on the cell surface. It plays a crucial role in many biological processes, and hypersialylation is a common feature in cancer. Lectins are widely used to analyze the cell surface expression of SA. However, these protein molecules are usually expensive and easily denatured, which calls for the development of alternative glycan-specific receptors and cell imaging technologies. In this study, SA-imprinted fluorescent core-shell molecularly imprinted polymer particles (SA-MIPs) were employed to recognize SA on the cell surface of cancer cell lines. The SA-MIPs improved suspensibility and scattering properties compared with previously used core-shell SA-MIPs. Although SA-imprinting was performed using SA without preference for the alpha-2,3- and alpha-2,6-SA forms, we screened the cancer cell lines analyzed using the lectins Maackia Amurensis Lectin I (MAL I, alpha-2,3-SA) and Sambucus Nigra Lectin (SNA, alpha-2,6-SA). Our results show that the selected cancer cell lines in this study presented a varied binding behavior with the SA-MIPs. The binding pattern of the lectins was also demonstrated. Moreover, two different pentavalent SA conjugates were used to inhibit the binding of the SA-MIPs to breast, skin, and lung cancer cell lines, demonstrating the specificity of the SA-MIPs in both flow cytometry and confocal fluorescence microscopy. We concluded that the synthesized SA-MIPs might be a powerful future tool in the diagnostic analysis of various cancer cells. Basel MDPI 2022 Cancers 14 8 urn:nbn:de:kobv:b43-546625 110.3390/cancers14081875 https://creativecommons.org/licenses/by/4.0/deed.de 2022-04-20 OPUS4-54018 Zeitschriftenartikel Lin, R.; Li, X.; Krajnc, A.; Li, Z.; Li, M.; Wang, W.; Zhuang, L.; Smart, S.; Zhu, Z.; Appadoo, D.; Harmer, J. R.; Wang, Z.; de Oliveira Guilherme Buzanich, Ana; Beyer, S.; Wang, L.; Mali, G.; Bennett, T. D.; Chen, V.; Hou, J. Mechanochemically Synthesised Flexible Electrodes Based on Bimetallic Metal-Organic Framework Glasses for the Oxygen Evolution Reaction The melting behaviour of metal-organic frameworks (MOFs) has aroused significant research interest in the areas of materials science, condensed matter physics and chemical engineering. This work first introduces a novel method to fabricate a bimetallic MOF glass, through meltquenching of the cobalt-based zeolitic imidazolate Framework (ZIF) [ZIF-62(Co)] with an adsorbed ferric coordination complex. The high-temperature chemically reactive ZIF-62-(Co) liquid facilitates the formation of coordinative bonds between Fe and imidazolate ligands, incorporating Fe nodes into the framework after quenching. The resultant Co-Fe bimetallic MOF glass therefore shows a significantly enhanced oxygen evolution reaction performance. The novel bimetallic MOF glass, when combined with the facile and scalable mechanochemical synthesis technique for both discrete powders and surface coatings on flexible substrates, enables significant opportunities for catalytic device Assembly Wiley 2022 Angewandte Chemie Int. Ed. 61 4 e202112880 10.1002/anie.202112880 2021-12-13 OPUS4-53209 Zeitschriftenartikel Chapartegui-Arias, Ander; Raysyan, A.; Belenguer, A. M.; Jaeger, Carsten; Tchipilov, Teodor; Prinz, Carsten; Abad Andrade, Carlos Enrique; Beyer, S.; Schneider, Rudolf; Emmerling, Franziska Tailored mobility in a zeolite imidazolate framework (ZIF) antibody conjugate Zeolitic imidazolate framework (ZIF) hybrid fluorescent nanoparticles and ZIF antibody conjugates have been synthesized, characterized, and employed in lateral-flow immunoassay (LFIA). The bright fluorescence of the conjugates and the possibility to tailor their mobility gives a huge potential for diagnostic assays. An enzyme-linked immunosorbent assay (ELISA) with horseradish peroxidase (HRP) as label, proved the integrity, stability, and dispersibility of the antibody conjugates, LC-MS/MS provided evidence that a covalent link was established between these metal-organic frameworks and lysine residues in IgG antibodies. Weinheim Wiley-VCH 2021 Chemistry - a European journal 27 36 9414 9421 urn:nbn:de:kobv:b43-532096 10.1002/chem.202100803 https://creativecommons.org/licenses/by/4.0/deed.de 2021-09-14 OPUS4-51723 Zeitschriftenartikel de Oliveira Guilherme Buzanich, Ana; Kulow, Anicó; Kabelitz, Anke; Grunewald, C.; Seidel, R.; Chapartegui-Arias, Ander; Radtke, Martin; Reinholz, Uwe; Emmerling, Franziska; Beyer, S. Observation of early ZIF-8 crystallization stages with X-ray absorption spectroscopy The present study investigates early stages of ZIF-8 crystallization up to 5 minutes post mixing of precursor solutions. Dispersive X-ray Absorption Spectroscopy (DXAS) provides a refined understanding of the evolution of the coordination environment during ZIF-8 crystallization. Linear Combination Fiting (LCF) suggests tetrakis(1-methylimidazole)zinc2+ to be a suitable and stable mononuclear structure analogue for some early stage ZIF-8 intermediates. Our results pave the way for more detailed studies on physico-chemical aspects of ZIF-8 crystallization to better control tailoring ZIF-8 materials for specific applications. Royal Scociety of Chemistry 2020 Soft Matter 17 2 331 334 10.1039/D0SM01356K 2020-12-03 OPUS4-52521 Zeitschriftenartikel Kimani, Martha Wamaitha; Beyer, S.; El-Schich, Z.; Gawlitza, Kornelia; Rurack, Knut; Gjörloff-Wingren, A. Imprinted Particles for Direct Fluorescence Detection of Sialic Acid in Polar Media and on Cancer Cells with Enhanced Control of Nonspecific Binding Glycoproteins are abundant on the cell surface of mammals, providing structural support, modulating cell Membrane properties, and acting as signaling agents. Variation of glycosylation patterns has been found to indicate various disease states, including cell malignancy. Sialic acid (SA) is present as a terminating group on cell-surface glycans, and its overexpression has been linked to several types of cancer. Detection of SA on the cell surface is therefore critical for detection of cancer in its early stages. In this work, a fluorescent molecularly imprinted polymer layer targeting SA was synthesized on the surface of silica-coated polystyrene (PS) particles. Compared to previous works, a PS core supplies a lighter, lower-density support for improved suspension stability and scattering properties. Moreover, their smaller size provides a higher surface-area-to-volume ratio for binding. The incorporation of a fluorescent monomer in the MIP shell allowed for simple and rapid determination of binding specificity in polar media due to a deprotonation−reprotonation interaction mechanism between the fluorescent monomer and SA, which led to spectral changes. Upon titration of the MIP particles with SA in suspension, an increase in fluorescence emission of the particles was observed, with the MIP particles binding SA more selectively compared to the nonimprinted polymer (NIP) control particles. In cell staining experiments performed by flow cytometry, the binding behavior of the MIP particles compared favorably with that of SA-binding lectins. NIPs prepared with a "dummy" template served as a better negative control in cell binding assays due to the favorable inward orientation of template-binding functional groups in the polymer shell, which reduced nonspecific binding. The results show that fluorescent MIPs targeting SA are a promising tool for in vitro fluorescence staining of cancerous cells and for future diagnosis of cancer at early stages. American Chemical Society 2021 ACS Applied Polymer Materials 3 5 2363 2373 urn:nbn:de:kobv:b43-525216 10.1021/acsapm.0c01353 https://creativecommons.org/licenses/by-nc-nd/4.0/deed.de 2021-04-28 OPUS4-47207 Zeitschriftenartikel Kulow, Anicó; Witte, S.; Beyer, S.; de Oliveira Guilherme Buzanich, Ana; Radtke, Martin; Reinholz, Uwe; Riesemeier, H.; Streli, C. A new experimental setup for time- and laterally-resolved X-ray absorption fine structure spectroscopy in a 'single shot' In this work, a new setup for dispersive XAFS measurements is presented. This reproducible and scanningfree setup yields both time- and laterally-resolved XAFS experiments in a 'single-shot'. It allows a straightforward adjustment for probing different elements covering many relevant applications in materials science. An incoming energetic broadband beam is diffracted by a Si (111) crystal after passing through the sample and collected by an area sensitive detector. Depending on the energy range of the incoming beam, XANES and/or EXAFS spectra can be recorded with a time resolution down to 1 s. The feasibility of this setup was demonstrated at the BAMline at BESSY II (Berlin, Germany) with reference Fe and Cu foils and the results are hereby presented and discussed. Additionally, an application where time resolution on the second scale is required is briefly evaluated. The presented example concerns studying early stages of zinc(II)2-methylimidazolate (ZIF-8) crystallization. This is particularly important for biomedical applications. London Royal Society of Chemistry 2019 Journal of Analytical Atomic Spectrometry 34 1 239 246 10.1039/c8ja00313k 2019-01-22