TY  - CONF
A1  - Konthur, Zoltán
T1  - Enzyme-Based Electrochemical Detection of Fumonisins Applying Amperometric Sensing
N2  - Fumonisins represent a class of toxic secondary metabolites produced primarily by Fusarium species, notably F. verticillioides and F. proliferatum, though Aspergillus niger can also produce these mycotoxins. They are most commonly found contaminating maize, but other grains are also at risk. Dietary exposure to fumonisins poses serious health risks to both humans and livestock, prompting the European Commission to set regulatory limits on their presence in food and animal feed. Traditional detection methods, such as chromatography, are often costly, are laboratory based and require specialized expertise. To address the need for simpler, more accessible detection methods, portable electrochemical biosensors present a promising solution. In this study, we introduce a novel enzymatic approach for fumonisin detection using amperometric sensing. Here, a recently discovered fumonisin amine oxidase (AnFAO) from Aspergillus niger, which catalyses the oxidative deamination of fumonisins and generates hydrogen peroxide, was produced recombinantly in Escherichia coli. In our hands, the enzyme exhibited higher specific activity toward Fumonisin B1 compared to Fumonisin B2 at the same substrate concentration (0.0037 U/mg and 0.0014 U/mg at 25 µM substrate, respectively). We demonstrated that enzyme activity correlates with both enzyme and substrate concentrations. To detect Fumonisin B1, the enzyme was covalently attached to magnetic particles, and hydrogen peroxide production was measured amperometrically in a flow injection system with Prussian blue carbon electrodes. This method enabled the quantification of Fumonisin B1 concentrations as low as 1.5 µM and proved that recombinant AnFAO effectively deaminated fumonisins even in its immobilized form. These results highlight AnFAO’s potential for developing an enzyme-based electrochemical biosensor to detect fumonisin contamination in food and feed products.
T2  - ANAKON 2025
CY  - Leipzig, Germany
DA  - 10.03.2025
KW  - Immunoassay
KW  - Mycotoxin
KW  - Electrochemistry
KW  - Antibody
KW  - Biosensor
PY  - 2025
AN  - OPUS4-63496
LA  - eng
AD  - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER  -