TY  - CONF
A1  - Zhang, Y.
T1  - Fluorescent molecularly imprinted polymers (MIPs) as novel tools for detection of sialic acid on tumor cells
N2  - Cancer is the second leading cause of death globally according to the WHO 2018. Lung, prostate, colorectal, stomach and liver cancer are the most common types of cancer in men, while breast, colorectal, lung, cervix and thyroid cancer are the most common among women. Sialic acid (SA) plays an important role in a variety of biological processes in cells. Tumor cells express high levels of SA, which is often associated with poor prognosis due to increased invasive potential. In this study, we have performed a screening of SA-MIPs binding to different cancer cell lines. The overall aim is to use the SA-MIPs for detection of tumor cells, analyzed by flow cytometry and fluorescence microscopy. To confirm the levels of SA expression on the investigated cell lines, we started out by analyzing the binding of the lectins MAL I and SNA. The staining pattern of the two lectins binding to either α-2,3 or α-2,6 linkage SA, respectively, was compared with the staining pattern of the SA-MIPs. We show that the different cell types analyzed have a varying pattern of SA-MIP binding. The comparison with lectin binding will be further evaluated.
T2  - Biomarkers – methods and technologies
CY  - Malmö, Sweden
DA  - 25.10.2018
KW  - Cancer
KW  - Sialic acid
KW  - MIPs
PY  - 2018
AN  - OPUS4-46492
LA  - eng
AD  - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER  -