TY  - JOUR
A1  - Gorris, H.H.
A1  - Resch-Genger, Ute
T1  - Perspectives and challenges of photon-upconversion nanoparticles - Part II: Bioanalytical applications
JF  - Analytical and Bioanalytical Chemistry
N2  - In Part II of this Review series on lanthanide-doped photon-upconversion nanoparticles (UCNPs), we present and critically discuss the Performance and suitability of UCNPs as background-free luminescent Reporters in bioimaging and bioanalytical applications. The preparation of a biocompatible nanoparticle surface is an integral step for all life - science-related applications. UCNPs have found their way into a large number of diagnostic platforms, homogeneous and heterogeneous assay formats, and sensor applications. Many bioanalytical detection schemes involve Förster resonance energy transfert (FRET), which is still debated for UCNPs and Needs to be much improved. The Need for dedicated and standardized instruments as well as recent studies on the Dissolution and potential toxicity of UCNPs are addressed. Finally we outline future Trends and challenges in the field of upconversion.
KW  - Fluorescence
KW  - Lanthanide
KW  - Upconversion
KW  - Brightness
KW  - Quantification
KW  - Nanoparticle
KW  - Absolute fluorometry
KW  - NIR
KW  - IR
KW  - Quantum yield
KW  - Assay
KW  - Bioconjugation
KW  - Imaging
KW  - FRET
KW  - Sensor
PY  - 2017
DO  - https://doi.org/10.1007/s00216-017-0482-8
SN  - 1618-2650
SN  - 1618-2642
VL  - 409
IS  - 25
SP  - 5875
EP  - 5890
PB  - Springer
AN  - OPUS4-41706
LA  - eng
AD  - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany
ER  -