TY - CONF A1 - Ecke, Alexander A1 - Bohm, Konrad A1 - Schneider, Rudolf T1 - Magnetic Bead-Based Immunoassays for Online Sensing Applications N2 - The demand for quick and reliable online sensors is rising not only in the field of environmental analysis with new and diversifying sources of pollution. A major aspect of this is the contamination of (drinking) water with pharmaceuticals representing a potential health threat and requiring timely countermeasures in case of contamination. In this regard, antibody-based methods can be helpful as they enable fast and cost-effective on-site and potentially online analyses. The transition from the conventional plate-based formats like ELISA (enzyme-linked immunosorbent assay) to an immunosensor fit for online sensing can be achieved by using magnetic microparticles as a mobile sensing platform. Beads are prepared by either coupling the antibody or the analyte to the surface. Both these strategies are presented using the example of two relevant contaminants in water: diclofenac (DCF) & amoxicillin (AMX), and can be transferred to other analytes. The developed magnetic bead-based assays (MBBAs) exhibit lower LODs, enhanced sensitivity as well as shorter time of analysis than the respective ELISAs and can be integrated into an immunosensor with electrochemical detection on a microfluidic chip. T2 - European Biosensor Symposium 2021 CY - Online meeting DA - 09.03.2021 KW - Immunoassay KW - Magnetic Beads KW - Diclofenac KW - Amoxicillin KW - Online Sensing PY - 2021 AN - OPUS4-52285 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -