TY - CONF A1 - Schwaar, Timm A1 - van den Berg, J. W. A1 - Abbas, Ioana M. A1 - Weller, Michael G. T1 - Particle-based epitope analysis of a monoclonal antibody N2 - A method for the examination of the epitope of a monoclonal antibody against a peptide related to hepcidin-25, known as a biomarker in the ion metabolism, was developed. Characterizing the exact binding site helps to understand the properties of the antibody. A peptide consists of different amino acids, each contributing differently to the binding strength. To examine the influence of the amino acids, each amino acid was subsequently exchanged by glycine. If the respective amino acid is critical for binding to the antibody, a significant drop in the binding affinity should be observed. The epitope screening of monoclonal antibodies is a complex and elaborate procedure. For this approach, variants of the peptides were synthesized by conventional Fmoc-based peptide synthesis. The solid support was chosen to be suitable for the synthesis and additional screening against the antibody. To determine the binding affinity, the bead-bound peptides were incubated with a fluorophore-labeled antibody. After the incubation, the beads were placed on a slide and the fluorescence was detected by a fluorescence scanner. A high fluorescence indicates a high binding affinity and vice versa. T2 - Tag der Chemie 2017 CY - Berlin, Germany DA - 05.07.2017 KW - Antibody KW - Epitope analysis PY - 2017 AN - OPUS4-41191 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -