TY - JOUR A1 - Löhr, Konrad A1 - Borovinskaya, O. A1 - Tourniaire, G. A1 - Panne, Ulrich A1 - Jakubowski, Norbert T1 - Arraying of single cells for quantitative high throughput laser ablation ICP-TOF-MS T2 - Analytical Chemistry N2 - Arraying of single cells for mass spectrometric analysis is a considerable bioanalytical challenge. In this study, we employ a novel single cell arraying technology for quantitative analysis and isotopic fingerprinting by laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOF-MS). The single cell arraying approach is based on a piezo-acoustic microarrayer with software for automated optical detection of cells within the piezo dispense capillary (PDC) prior to arraying. Using optimized parameters, single cell occupancy of >99%, high throughput (up to 550 cells per hour), and a high cell recovery of >66% is achieved. LA-ICP-TOF-MS is employed to detect naturally occurring isotopes in the whole mass range as fingerprints of individual cells. Moreover, precise quantitative determination of metal-containing cell dyes is possible down to contents of ∼100 ag using calibration standards which were produced using the same arrayer. PB - American Chemical Society (ACS Publications) CY - Washington KW - Laser ablation KW - Cell KW - Array KW - ICP-MS PY - 2019 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/48985 AN - OPUS4-48985 SN - 0003-2700 SN - 1520-6882 VL - 91 IS - 18 SP - 11520 EP - 11528 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany