TY - JOUR A1 - Heck, Christian A1 - Kanehira, Y. A1 - Kneipp, Janina A1 - Bald, Ilko T1 - Placement of single proteins within the SERS hot spots of self-assembled silver nanolenses JF - Angewandte Chemie International Edition N2 - This study demonstrates the bottom-up synthesis of silver nanolenses.Arobust coating protocol enabled the functionalization of differently sized silver nanoparticles with DNAsingle strands of orthogonal sequence.Coated particles 10 nm, 20 nm, and 60 nm in diameter were self-assembled by DNAorigami scaffolds to form silver nanolenses.Single molecules of the protein streptavidin were selectively placed in the gap of highest electric field enhancement. Streptavidin labelled with alkyne groups served as model analyte in surface- enhanced Raman scattering (SERS) experiments.Bycorre- lated Raman mapping and atomic force microscopy, SERS signals of the alkyne labels of asingle streptavidin molecule, from asingle silver nanolens,were detected. The discrete,self- similar aggregates of solid silver nanoparticles are promising for plasmonic applications. KW - DNA origami KW - Surface enhanced Raman scattering KW - Protein KW - Single molecule KW - Nanotechnology PY - 2018 DO - https://doi.org/10.1002/anie.201801748 SN - 1433-7851 VL - 57 IS - 25 SP - 7444 EP - 7447 PB - WILEY AN - OPUS4-45743 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -