TY - CONF A1 - Muth, Thilo T1 - Benchmarking the current state of metaproteomics : a community driven evaluation of experimental and computational techniques N2 - Metaproteomics has substantially grown over the past years and supplements other omics approaches by bringing valuable functional information, enabling genotype- phenotype linkages and connections to metabolic outputs. Currently, a wide variety of metaproteomic workflows is available, yet their impact on the results remains to be thoroughly assessed. Here, we carried out the first community-driven, multi-lab comparison in metaproteomics: the critical assessment of metaproteome investigation (CAMPI) study. Based on well-established workflows, we evaluated the influence of sample preparation, mass spectrometry acquisition, and bioinformatic analysis using two samples: a simplified, lab-assembled human intestinal model and a human fecal sample. Although bioinformatic pipelines contributed to variability in peptide identification, wet-lab workflows were the most important source of differences between analyses. Overall, these peptide-level differences largely disappeared at the protein group level. Differences were observed between peptide- and protein-centric approaches for the predicted community composition but similar functional profiles were found across workflows. The CAMPI findings demonstrate the robustness of current metaproteomics research and provide a perspective for future benchmarking studies. T2 - ISMB/ECCB 2021 CY - Online meeting DA - 28.07.2021 KW - Mass spectrometry KW - Metaproteomics KW - Benchmarking KW - International lab study KW - Bioanalytics PY - 2021 AN - OPUS4-56685 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -