TY - CONF A1 - You, Zengchao A1 - Winckelmann, Alexander A1 - Vogl, Jochen A1 - Recknagel, Sebastian A1 - Abad, Carlos T1 - Determination of calcium, iron, and selenium in human serum by isotope dilution analysis using MICAP-MS N2 - Trace elemental analysis in human serum is integral in both clinical and research settings. Analyzing the level of some specific elements like Se and Zn helps indicate the nutritional and health status. Furthermore, elucidating the roles of trace elements in various physiological and pathological conditions can shed light on disease mechanisms and potential treatments. Inductively coupled plasma mass spectrometry (ICP-MS) stands out as a preeminent method for trace elemental analysis, given its exceptional sensitivity and minimal sample requirements. However, ICP-MS has challenges, such as argon-related interferences that hinder the accurate quantification of elements like Ca, Fe, and Se. The naturally most abundant isotopes of Ca (40Ca), Fe (56Fe), and Se (80Se) are subject to isobaric interference from 40Ar+, 40Ar16O+, and 40Ar2+, thereby complicating their direct measurement and preventing isotope dilution analysis. In response to these challenges, nitrogen microwave inductively coupled atmospheric pressure mass spectrometry (MICAP-MS) emerges as a viable alternative to ICP-MS, eliminating argon consumption and associated interferences. This study employed MICAP-MS to quantify Ca, Fe, and Se in 11 certified reference human serums using matrix-matched calibration and isotope dilution with the isotopes 40Ca, 56Fe, and 80Se. The results obtained with both methods were validated against certified values, and the suitability of MICAP-MS for isotope dilution was evaluated. Additionally, the performance of MICAP-MS in the Na matrix was investigated and discussed alongside the impact of organic species. T2 - Winter Conference on Plasma Spectrochemistry 2024 CY - Tucson, AZ, USA DA - 15.01.2024 KW - MICAP-MS KW - Isotope-dilution KW - Human serum PY - 2024 AN - OPUS4-60210 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -