TY - GEN A1 - Makowski, Maike T1 - Hot-spots of non-volatile mycotoxins in cereals: How can we overcome this problem? N2 - Since cereals constitute a staple food worldwide, monitoring of growth, storage and processing represent a perseverative challenge of analytical sciences. Especially during storage infested grains breed fungal clusters (“hot spots”) in which mycotoxins accumulate and can highly exceed allowed maximum levels. Because of their unpredictable presence, analytical procedures – including representative sampling - contain invasive and time intensive steps. To avoid erroneously negative results in mycotoxin analysis in cereals, the samples´ homogeneous headspace might be investigated instead of analyzing subsamples of the grains. So-called microbial volatile organic compounds (MVOC´s) provide information for instance about the fungal status of grains. Previous investigations revealed trichodiene to be a precursor of trichothecene mycotoxins which comprises more than 180 different compounds. Due to its non-functionalised sesquiterpene structure, quantification of trichodiene using Headspace GC-MS methods is possible. Thereby, it could be used as biomarker for trichothecene contamination of cereals. Nevertheless, further investigations are necessary. To be able to draw conclusion about the trichothecene content in cereal samples, a possible correlation between trichodiene concentration in the gas phase and trichothecene concentration in the sample must be examined. Realizing this idea would widely extend the applicability of trichodiene and enormously simplify trichothecene detection. Hence, the first step of an ongoing study aims to develop a laboratory reference method using trichodiene as volatile biomarker to quantify trichothecenes in cereals. Both static headspace and SPME-enrichment coupled to gas chromatography with mass spectrometry (GC-MS) were tested and compared. In a second step, this reference method is intended to validate new approaches for fast on-site screening of trichodiene in cereals. T2 - ANAKON 2019 CY - Münster, Germany DA - 25.03.2019 KW - Trichodiene KW - Gaschromatography-Mass Spectrometry KW - Mycotoxin-Biomarker PY - 2019 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/47847 AN - OPUS4-47847 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany