TY - GEN A1 - Ecke, Alexander T1 - Development of an ELISA for the determination of amoxicillin in water samples N2 - We report the first results for the immunochemical determination of the β-lactam antibiotic drug amoxicillin (AMX) in water samples by ELISA (enzyme-linked immunosorbent assay). The applied indirect competitive ELISA using a commercially available mouse anti-AMX antibody enables testing for AMX with a limit of detection of around 1 µg/L. Calibration curves of calibrators prepared in different water samples showed equal shape. This underlines the suitability of this assay for the AMX determination in these samples. Furthermore, cross-reactivities of structurally similar β-lactam antibiotics were examined and found to be low for ampicillin, penicillin G and penicillin V. In contrast, carbenicillin was recognized by the antibody even better than AMX indicating a positive effect of an additional carboxyl group on antibody binding. This is promising for further application of this assay, e.g. in the determination of the most relevant hydrolysis product of AMX, amoxicilloic acid. T2 - Berliner Chemie Symposium CY - Berlin, Germany DA - 26.09.2019 KW - Amoxicillin KW - Immunoassay KW - Water Analysis KW - Antibiotics PY - 2019 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/49178 AN - OPUS4-49178 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany