TY - JOUR A1 - Zavoiura, Oleksandr A1 - Resch-Genger, Ute A1 - Seitz, Oliver T1 - Quantum dot-PNA conjugates for target-catalyzed RNA detection JF - Bioconjugate Chemistry N2 - Detection of pathogenic nucleic acids remains one of the most reliable approaches for the diagnosis of a broad range of diseases. Current PCR-based methods require experienced personnel and cannot be easily used for point-of care diagnostics, making alternative strategies for the sensitive, reliable, and cost-efficient detection of pathogenic nucleic acids highly desirable. Here, we report an enzyme-free method for the fluorometric detection of RNA that relies on a target-induced fluorophore transfer onto a semiconductor quantum dot (QD), uses PNA probes as selective recognition elements and can be read out with simple and inexpensive equipment. For QD-PNA conjugates with optimized PNA content, limits of detection of dengue RNA in the range of 10 pM to 100 nM can be realized within 5 h in the presence of a high excess of noncomplementary RNA. KW - FRET KW - Fluorescence KW - DNA KW - Assay KW - Quantum dot KW - Nano KW - Particle KW - Synthesis KW - Ligation Assay PY - 2018 DO - https://doi.org/10.1021/acs.bioconjchem.8b00157 SN - 1043-1802 VL - 29 IS - 5 SP - 1690 EP - 1702 PB - ACS Publications AN - OPUS4-45120 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -