TY - GEN A1 - Kjaervik, Marit T1 - Characterization of biofilms with near ambient pressure XPS N2 - Near Ambient Pressure XPS opens up a new world of possibilities for measurements with XPS. While there are examples where NAP-XPS has been used to study electrochemical processes and heterogeneous catalysis, little attention has been paid to its potential use in biological materials. Until now, bacteria have only been characterised with conventional XPS, which requires tedious sample preparation usually involving freeze drying, a treatment that may degrade biological sample constituents. By studying biological samples in their native wet states, new insight about composition, absorption and transport of drugs through cell membranes and extracellular polymeric substance (EPS) layers can be obtained. Both artificial model-films of exopolysaccharides and biofilms of Escherichia Coli have been characterised at pressures ranging from ultra-high vacuum to 15 mbar by using SPECS’ EnviroESCA NAP-XPS instrument and conventional XPS. By applying antimicrobials to model biofilms, some of which are known to be resistant towards the antimicrobial in question, the distribution of antimicrobials in biofilms has been studied. Capabilities and limitations of the approach will be discussed. T2 - The 4th annual workshop on ambient presssure X-ray photoelectron spectroscopy CY - Shanghai, China DA - 13.12.2017 KW - NAP-XPS KW - Biofilms KW - E. coli PY - 2017 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/43568 AN - OPUS4-43568 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany