TY - GEN A1 - Schmidt, Selina T1 - Priming in soil microbial communities mediated by biocide-induced horizontal gene-transfer N2 - Soil microbes are exposed to different environmental stressors originating from various sources. Biocides used as material preservatives can represent environmental stressors since they are in direct contact with the environment including soil. Microorganism in soils can adapt to stress by different mechanisms; for example, by transferring mobile genetic elements via horizontal gene transfer (HGT). Here, we hypothesize that material preservatives can cause increased frequencies of HGT (i.e. altered community permissiveness) facilitating microbial community adaptation to stress. Furthermore, we hypothesize that soil microbial communities are primed by biocide exposure facilitating the response to different types of stresses. We will incubate soil mesocosms with selected biocides to investigate if these compounds promote HGT of plasmids that carry resistance genes in soil microbial communities. Subsequently, we will prime the soil microbial community with sub-inhibitory concentrations of biocides followed by exposure to toxic biocide concentrations or other types of common environmental stresses including metals, antibiotics, and salt. Using suitable control experiments, a shift in the functional response of the primed as compared to the non-primed community would indicate that biocides prime microbial communities via HGT. Together these results will elucidate the effect of biocides on HGT-mediated adaptation to environmental stressors in soil microbial communities. T2 - SFB973 Stress Symposium CY - Berlin, Germany DA - 09.04.2018 KW - Resistance evolution KW - Horizontal gene transfer HGT KW - Biocides KW - Microbiology PY - 2018 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/46285 AN - OPUS4-46285 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany