TY - JOUR A1 - Hoffmann, Holger A1 - Baldofski, Stefanie A1 - Hoffmann, Kristin A1 - Flemig, Sabine A1 - Silva, C. P. A1 - Esteves, V. I. A1 - Emmerling, Franziska A1 - Panne, Ulrich A1 - Schneider, Rudolf T1 - Structural considerations on the selectivity of an immunoassay for sulfamethoxazole T2 - Talanta N2 - Sulfamethoxazol (SMX),a sulfonamide, is a widely used bacteriostatic antibiotic and therefore a promising marker for the entry of anthropogenic Pollution in the environment. SMX is frequently found in wastewater and surface water. This study presents the production of high affinity and selective polyclonal antibodies for SMX and the development and Evaluation of a direct competitive enzyme-linked immunosorbent assay(ELISA)for the quantification of SMX in environmental watersamples. The crystal structures of the cross-reacting compounds sulfamethizole, N4-acetyl-SMX andsuccinimidyl-SMX were determined by x-ray diffraction aiming to explain their high cross-reactivity. These crystal structures are described for the first time. The quantification range of the ELISA is 0.82–63 µg/L. To verify our results, the SMX concentration in 20 environmental samples,including wastewater and surfacewater,was determined by ELISA and tandem mass spectrometry(MS/MS).A good Agreement of the measured SMX concentrations was found with average recoveries of 97–113%for the results of ELISA compared to LC-MS/MS. PB - Elsevier B.V. CY - Amsterdam, Netherlands KW - X-Ray diffraction KW - Sulfamethoxazole KW - ELISA KW - LC-MS/MS PY - 2016 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/36676 AN - OPUS4-36676 SN - 0039-9140 SN - 1873-3573 IS - 158 SP - 198 EP - 207 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany