TY - JOUR A1 - Neuhof, Torsten A1 - Ganzauer, Natalie A1 - Koch, Matthias A1 - Nehls, Irene T1 - A comparison of chromatographic methods for the determination of deoxynivalenol in wheat JF - Chromatographia N2 - Liquid chromatography coupled with tandem mass spectrometric detection has gained more importance for mycotoxin determination in recent years. In addition to instrumental improvements, the development of LC-MS-MS has also been a consequence of the availability of stable isotope internal standards, e.g., 13C-labelled mycotoxins. Thus, the LC-ESI-MS-MS method using a 13C15-deoxynivalenol internal standard as a state-of-the-art technique was selected as a reference procedure for an in-house method comparison study of the determination of deoxynivalenol (DON) in wheat materials. Alternative methods include LC-diode array detection, which is a frequently used routine procedure for DON-analysis, and gas chromatography after trimethylsilylation. For GC application, an electron capture detector and a negative chemical ionisation mass spectrometry detector were used, which have both been well described in the literature. The method comparison was conducted using t test statistics. Additionally, this study also calculates important method performance characteristics, including accuracy, linearity, limit of detection, limit of quantification, recovery, and variation coefficient. Furthermore, this is the first report of a GC-MS method for the determination of DON using a fully carbon-labelled 13C15-DON as an IS. The GC-MS using 13C15-DON as an IS has produced comparable results to the 13C-IS-LC-MS-MS reference method with a similar sensitivity. ECD detection was slightly less sensitive, but is also suited for DON analysis in wheat. Due to the high LOQ, the LC-DAD method seems highly applicable to the measurement of highly contaminated samples. KW - Column liquid chromatography-mass spectrometry KW - Gas chromatography KW - Mycotoxins KW - Trichothecenes PY - 2009 DO - https://doi.org/10.1365/s10337-009-1084-1 SN - 0009-5893 SN - 1612-1112 VL - 69 IS - 11-12 SP - 1457 EP - 1462 PB - Vieweg CY - Wiesbaden AN - OPUS4-19618 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -