TY - JOUR A1 - Grabolle, Markus A1 - Kapusta, P. A1 - Nann, T. A1 - Shu, X. A1 - Ziegler, J. A1 - Resch-Genger, Ute T1 - Fluorescence lifetime multiplexing with nanocrystals and organic labels JF - Analytical chemistry N2 - The potential of semiconducting nanocrystals or so-called quantum dots (QDs) for lifetime multiplexing has not been investigated yet, despite the increasing use of QDs in (bio)analytical detection, biosensing, and fluorescence imaging and the obvious need for simple and cost-effective tools and strategies for the simultaneous detection of multiple analytes or events. This is most likely related to their multiexponential decay behavior as for multiplex chromophores, typically monoexponential decay kinetics are requested. The fluorescence decay kinetics of various mixtures of a long-lived, multiexponentially decaying CdSe QD and a short-lived organic dye were analyzed, and a model was developed for the quantification of these labels from the measured complex decay kinetics as a first proof-of-concept for the huge potential of these labels for lifetime multiplexing. In a second step, we evaluated the potential of mixtures of two types of QDs, varying in constituent material to realize distinguishable, yet multiexponential decay kinetics and similar absorption and emission spectra. Strategies for lifetime multiplexing with nanocrystalline labels were derived on the basis of these measurements. KW - Multiplexing KW - Lifetime KW - Fluorescence KW - Photoluminescence KW - TCSPC KW - Quantum dot KW - Nanocrystal KW - CdSe KW - InP KW - Organic dye KW - DCM PY - 2009 DO - https://doi.org/10.1021/ac900934a SN - 0003-2700 SN - 1520-6882 VL - 81 IS - 18 SP - 7807 EP - 7813 PB - American Chemical Society CY - Washington, DC AN - OPUS4-20279 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -