TY - CONF A1 - Burnage, Samual Charles A1 - Gawlitza, Kornelia A1 - Sarma, Dominik A1 - Wan, Wei A1 - Bell, Jérémy A1 - Rurack, Knut T1 - Combination of Fluorescent Molecularly Imprinted Polymers and Microfluidics for the Detection of Cancer Biomarker Phosphorylated Tyrosine N2 - Cancer is modern medicine’s biggest challenge. It is now thought to be responsible for one in six deaths worldwide making early diagnosis, and treatment thereon, essential for better prognoses1. Protein phosphorylation is a post-translational modification of particular interest as a biomarker in the understanding of neurodegenerative diseases and a number of cancer pathways. There is therefore a need for robust, fast and low-cost techniques for the detection of these phosphorylations. Fluorescent molecularly imprinted polymers (MIPs) are a cheap and selective material for both the extraction and detection of a multitude of analytes. Often referred to as “plastic antibodies”, MIPs provide added robustness and chemical stability compared to their natural counterparts. Application of these fluorescent MIPs to a microfluidic lab-on-a-chip platform offers a fast, versatile method for the detection of biomarkers containing phosphorylated amino-acids such as phosphorylated tyrosine. Here, we present a tuneable core-shell MIP system consisting of a polystyrene core, silica inner-shell and MIP outer-shell. The MIP outer-shell contains a urea-based fluorescent probe monomer co-polymerised into the polymer matrix that can detect phosphorylated-tyrosine based on a change in its optical properties. The phosphate group interacts with the fluorescent probe via hydrogen bonding interactions yielding a fluorescence enhancement in organic solvents. The fluorescent MIPs are to be applied to a microfluidic platform, for rapid extraction of the analyte from the aqueous sample phase and simple optical detection in the organic phase that contains the MIP microparticles. T2 - Rapid Methods Europe 2018 (RME2018) CY - Amsterdam, The Netherlands DA - 05.11.2018 KW - Molecularly Imprinted Polymers KW - Microfluidics KW - Fluorescent MIPs KW - MIPs KW - Phosphorylated Tyrosine KW - Biomarker PY - 2018 AN - OPUS4-47019 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -