TY - JOUR A1 - Mönch, Bettina A1 - Becker, Roland A1 - Jung, Christian A1 - Nehls, Irene T1 - The homogeneity testing of EtG in hair reference materials: A high-throughput procedure using GC-NCI-MS T2 - Forensic science international N2 - The validation of a robust quantification procedure for EtG in hair using GC–NCI–MS is presented. Aqueous extraction is followed by complete lyophylization of the extract and derivatization with pentafluoropropionic anhydride (PFPA) under controlled temperature and duration. Clean-up of extracts was dispensable and standard single quadrupole MS displayed sufficient selectivity and sensitivity. The method displayed a wide linearity range and enabled LOD of 0.68 pg/mg, LOQ of 2.4 pg/mg, and precision below 8.12%. Since EtG was seen to display prolonged stability in the aqueous extracts and after derivatization with PFPA this straightforward procedure allows a routine throughput of large quantities of samples with little proneness to procedural scatter of results. The method was applied to demonstrate the homogeneity of two hair reference materials with mean EtG contents of 8.48 pg/mg and 22.0 pg/mg. Aside from the application in homogeneity studies of hair reference materials predominantly in the concentration range of 10–50 pg/mg the method was also designed for daily routine quantification of real-world sample with regard to drinking behavior assessment. PB - Elsevier Ltd. CY - Amsterdam [u.a.] KW - Extraction KW - Derivatization KW - High-throughput analysis KW - Reference materials KW - Homogeneity KW - Measurement uncertainty PY - 2013 UR - https://opus4.kobv.de/opus4-bam/frontdoor/index/index/docId/27998 AN - OPUS4-27998 SN - 0379-0738 SN - 1872-6283 VL - 226 IS - 1-3 SP - 202 EP - 207 AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany