TY - JOUR A1 - Olejko, L. A1 - Cywinski, P.J. A1 - Bald, Ilko T1 - Ion-selective formation of a guanine quadruplex on DNA origami structures JF - Angewandte Chemie / International Edition N2 - DNA origami nanostructures are a versatile tool that can be used to arrange functionalities with high local control to study molecular processes at a single-molecule level. Here, we demonstrate that DNA origami substrates can be used to suppress the formation of specific guanine (G) quadruplex structures from telomeric DNA. The folding of telomeres into G-quadruplex structures in the presence of monovalent cations (e.g. Na+ and K+) is currently used for the detection of K+ ions, however, with insufficient selectivity towards Na+. By means of FRET between two suitable dyes attached to the 3'- and 5'-ends of telomeric DNA we demonstrate that the formation of G-quadruplexes on DNA origami templates in the presence of sodium ions is suppressed due to steric hindrance. Hence, telomeric DNA attached to DNA origami structures represents a highly sensitive and selective detection tool for potassium ions even in the presence of high concentrations of sodium ions. KW - DNA nanotechnology KW - FRET KW - G-quadruplexes KW - Nanostructures KW - Self-assembly KW - DNA origami KW - Fluorescence spectroscopy KW - Sensing PY - 2014 UR - http://onlinelibrary.wiley.com/doi/10.1002/anie.201409278/full DO - https://doi.org/10.1002/anie.201409278 SN - 1433-7851 SN - 1521-3773 SN - 0570-0833 VL - 53 SP - 1 EP - 6 PB - Wiley-VCH CY - Weinheim AN - OPUS4-32196 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -