TY - CONF A1 - Schäferling, Michael A1 - Deguchi, T. A1 - Christ, S. A1 - Peltomaa, R. A1 - Prabhakar, N. A1 - Rosenholm, J. A1 - Arppe, Riika A1 - Soukka, T. A1 - Näreoja, T. T1 - Nanoprobes for sensing and imaging of intracellular pH based on polyethyleneimide-coated photon upconversion nanoparticles conjugated to a pH sensitive rhodamine dye N2 - Lanthanide-doped photon upconversion nanoparticles (UCNPs) exhibit many advantages compared to Stokes-shifted luminescent probes (organic dyes, quantum dots). Due to the upconversion process, the limitations of photobleaching, autofluorescence and low penetration depths in tissue shown by classical fluorescent probes are avoided. This makes UCNPs particularly useful for applications in complex biological samples. Sensing of intracellular pH is of particular interest in biomedical research since structure and function of biomolecules strongly depend on the concentration of protons in their environment. T2 - UPCON 2016 CY - Wroclaw, Poland DA - 23.05.2016 KW - upconversion KW - nanoprobes KW - chemical sensing PY - 2016 AN - OPUS4-37282 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -