TY - JOUR A1 - Linck, Lena A1 - Resch-Genger, Ute T1 - Identification of efficient fluorophores for the direct labeling of DNA via rolling circle amplification (RCA) polymerase phi29 JF - European journal of medicinal chemistry N2 - The enzymatic incorporation as well as the spectroscopic properties and photochemical stability of a series of fluorescent labels differing in dye class, charge, and rigidity were studied to identify new tools for signal enhancement in situ on microarrays without secondary labeling. These fluorophores were chosen to spectrally match or resemble the golden standard Cy3. With the rhodamine DY-555, that is three times more emissive than Cy3, we found a bright and stable chromophore, the spectroscopic properties of which are minimally influenced by dye microenvironment. KW - Rolling circle amplification (RCA) KW - DNA KW - Fluorescent label KW - Fluorescence quantum yield KW - Cyanine KW - Stability PY - 2010 DO - https://doi.org/10.1016/j.ejmech.2010.09.005 SN - 0009-4374 SN - 0223-5234 VL - 45 IS - 12 SP - 5561 EP - 5566 PB - EDIFOR CY - Paris AN - OPUS4-22470 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -