TY - JOUR A1 - Fretwurst, T. A1 - Buzanich, Günter A1 - Nahles, S. A1 - Woelber, J. P. A1 - Riesemeier, Heinrich A1 - Nelson, K. T1 - Metal elements in tissue with dental peri-implantitis: a pilot study JF - Clinical Oral Implants Research N2 - Objectives: Dental peri-implantitis is characterized by a multifactorial etiology. The role of metal elements as an etiological factor for peri-implantitis is still unclear. The aim of this study was to investigate the incidence of metal elements in bone and mucosal tissues around dental Grade 4 CP titanium implants with signs of peri-implantitis in human patients. Methods: In this prospective pilot study, all patients were enrolled consecutively in two study centers. Bone and soft tissue samples of patients with peri-implantitis with indication for explantation were analyzed for the incidence of different elements (Ca, P, Ti, Fe) by means of synchrotron radiation X-ray fluorescence spectroscopy (SRXRF) and polarized light microscopy (PLM). The existence of macrophages and lymphocytes in the histologic specimens was analyzed. Results: Biopsies of 12 patients (seven bone samples, five mucosal samples) were included and analyzed. In nine of the 12 samples (75%), the SRXRF examination revealed the existence of titanium (Ti) and an associated occurrence with Iron (Fe). Metal particles were detected in peri- implant soft tissue using PLM. In samples with increased titanium concentration, lymphocytes were detected, whereas M1 macrophages were predominantly seen in samples with metal particles. Conclusion: Titanium and Iron elements were found in soft and hard tissue biopsies retrieved from peri-implantitis sites. Further histologic and immunohistochemical studies need to clarify which specific immune reaction metal elements/particles induce in dental peri-implant tissue. KW - Peri-implantitis KW - Metal elements KW - Synchrotron radiation X-ray fluorescence PY - 2016 DO - https://doi.org/10.1111/clr.12718 SN - 0905-7161 VL - 27 IS - 9 SP - 1178 EP - 1186 PB - WILEY-BLACKWELL CY - 111 RIVER ST, HOBOKEN 07030-5774, NJ USA AN - OPUS4-38641 LA - eng AD - Bundesanstalt fuer Materialforschung und -pruefung (BAM), Berlin, Germany ER -